MRL/MpJ-Fas-KO Mouse
제품명
MRL/MpJ-Fas-KO Mouse
제품 ID
C001602
품종 계통
MRL/MpJ-Fasem1/Cya
Backgroud
MRL/MpJ
상태
이 마우스 계통을 논문에서 사용할 경우, “MRL/MpJ-Fas-KO Mouse (카탈로그 번호 C001602)은 Cyagen에서 구입하였습니다.”라고 명시해 주시기 바랍니다.
구매 가능한 제품 종류
연령
Genotype
성별
수량
유전자명
유전자 별칭
lpr, APO1, APT1, CD95, TNFR6, Tnfrsf6
NCBI ID
염색체
Chr 19
MGI ID
--
Datasheet
품종 계통 설명
The Fas cell surface death receptor (FAS), also known as CD95 or TNFRSF6, is a type I transmembrane protein in the tumor necrosis factor receptor superfamily [1]. It plays a key role in apoptosis by transmitting signals that initiate programmed cell death. Expressed across various tissues such as immune cells, liver, heart, and skin, FAS regulates cellular homeostasis. Upon binding with its ligand (FASL), FAS oligomerizes to form the death-inducing signaling complex (DISC), activating caspases that execute apoptosis. This is crucial for immune homeostasis, tissue maintenance, and removing damaged or infected cells. Dysregulated FAS-mediated apoptosis can lead to autoimmune disorders (e.g., autoimmune lymphoproliferative syndrome) or contribute to cancer development by enabling uncontrolled cell proliferation [1-3].
The MRL/MpJ Strain (Murphy Roths Large), derived from a complex cross between several strains (LG/J, AKR/J, C3H/Di, C57BL/6), was established for autoimmune disease research. They serve as controls in studies of the MRL/MpJ-Faslpr strain, a mouse model with premature termination of gene transcription and abnormal mRNA splicing due to lymphoproliferation (lpr) spontaneous mutation of the Fas gene, which develops lupus-like autoimmune disease [4]. MRL/MpJ mice are vital in studying autoimmune conditions (e.g., Sjögren syndrome, autoimmune arthritis, lupus erythematosus, hearing defects) and are notable for their scarless tissue regeneration capabilities. This makes them valuable models for research in tissue regeneration, wound healing, autoimmune diseases, muscle dystrophy, and hearing loss [4-6].
The MRL/MpJ-Fas knockout (KO) mouse model, generated via targeted deletion of the Fas gene in MRL/MpJ mice, provides a critical tool for investigating the role of FAS in autoimmune pathologies. This model furnishes a critical system for elucidating the etiology of systemic lupus erythematosus (SLE) and identifying potential therapeutic targets within the FAS signaling cascade for the treatment of autoimmune disorders.
Reference
Volpe E, Sambucci M, Battistini L, Borsellino G. Fas-Fas Ligand: Checkpoint of T Cell Functions in Multiple Sclerosis. Front Immunol. 2016 Sep 27;7:382.
Opferman JT. Apoptosis in the development of the immune system. Cell Death Differ. 2008 Feb;15(2):234-42.
de Oliveira GL, Malmegrim KC, Ferreira AF, Tognon R, Kashima S, Couri CE, Covas DT, Voltarelli JC, de Castro FA. Up-regulation of fas and fasL pro-apoptotic genes expression in type 1 diabetes patients after autologous haematopoietic stem cell transplantation. Clin Exp Immunol. 2012 Jun;168(3):291-302.
Adachi M, Watanabe-Fukunaga R, Nagata S. Aberrant transcription caused by the insertion of an early transposable element in an intron of the Fas antigen gene of lpr mice. Proc Natl Acad Sci U S A. 1993 Mar 1;90(5):1756-60.
Heydemann A. The super super-healing MRL mouse strain. Front Biol (Beijing). 2012 Dec 1;7(6):522-538.
Velasco C, Dunn C, Sturdy C, Izda V, Martin J, Rivas A, McNaughton J, Jeffries MA. Ear wound healing in MRL/MpJ mice is associated with gut microbiome composition and is transferable to non-healer mice via microbiome transplantation. PLoS One.
변형 전략
The sequence of exons 2-4 of the Fas gene was knocked out in MRL/MpJ mice by gene editing technology.
Figure 1. Gene editing strategy for MRL/MpJ-Fas-KO mice.
응용 분야
Systemic Lupus Erythematosus (SLE) research;
Immunology and inflammation-related research;
Other autoimmune research (Sjögren’s syndrome, Rheumatoid Arthritis, etc.).
문의하기
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