Ddx6-flox Mouse
Common Name
Ddx6-flox
제품 ID
S-CKO-02024
Backgroud
C57BL/6JCya
품종 계통계통 ID
CKOCMP-13209-Ddx6-B6J-VA
상태
이 마우스 계통을 논문에서 사용할 경우, “Ddx6-flox Mouse (카탈로그 번호 S-CKO-02024)은 Cyagen에서 구입하였습니다.”라고 명시해 주시기 바랍니다.
구매 가능한 제품 종류
연령
Genotype
성별
수량
표준 제공 조건은 최소 3마리의 이형접합(heterozygous) 보균자를 보장합니다. 동형접합(homozygous) 보균자 및/또는 특정 성별에 대한 브리딩 서비스도 제공됩니다.
기본 정보
품종 계통
Ddx6-flox
품종 계통계통 ID
CKOCMP-13209-Ddx6-B6J-VA
유전자명
제품 ID
S-CKO-02024
유전자 별칭
p54, rck, HLR2, mRCK/P54, 1110001P04Rik, C430015D01Rik, E230023J21Rik
배경
C57BL/6JCya
NCBI ID
변형 내용
Conditional knockout
염색체
Chr 9
Phenotype
Datasheet
적용 분야
--
품종 계통 설명
Ensembl 전사체 ID
ENSMUST00000170489
NCBI 전사체 ID
NM_001110826
타겟 영역
Exon 3~4
유효 영역 크기
~2.1 kb
유전자 연구 개요
Ddx6, also known as Rck/p54, is a member of the DEAD-box family of helicases highly conserved from unicellular eukaryotes to vertebrates. It is an essential component of cytoplasmic RNA-processing bodies (P-bodies) and plays a crucial role in multiple post-transcriptional processes such as mRNA storage, translational repression, and decay [3]. It is also involved in pathways like miRNA-mediated gene silencing, alternative translation initiation, and RNA editing [3,7]. In addition, Ddx6 has been implicated in the regulation of cell fate transitions, stress granule and P-body assembly, and immune-related regulatory networks, making it a key player in maintaining cellular homeostasis [1,2,4]. Genetic models, especially knockout (KO) and conditional knockout (CKO) mouse models, are valuable tools for studying its functions.
Suppression of Ddx6 in human and mouse primed embryonic stem cells endows them with a differentiation-resistant, “hyper-pluripotent” state and enables easy reprogramming to a naive state [1]. In adult progenitors, Ddx6 controls the balance between self-renewal and differentiation in a context-dependent manner [1]. Ddx6-silencing in non-immune cells suppresses the NF-κB pathway and inhibits activation of the IL-6 amplifier, while its overexpression enhances NF-κB promoter activity, suggesting its involvement in the pathogenesis of inflammatory diseases [5]. In pancreatic cancer, compared with adjacent tissues, Ddx6 expression is abnormally increased in human pancreatic cancer tissues, and overexpression promotes cancer cell proliferation and tumor formation, while knockdown has the opposite effects [6].
In conclusion, Ddx6 is a multifunctional regulator in post-transcriptional gene expression. Studies using KO/CKO mouse models have revealed its significance in cell fate determination, stress response, and the pathogenesis of inflammatory and cancerous diseases. Understanding Ddx6's functions provides insights into biological processes and may offer potential therapeutic targets for related diseases.
References：
1. Di Stefano, Bruno, Luo, En-Ching, Haggerty, Chuck, Yeo, Gene W, Hochedlinger, Konrad. 2019. The RNA Helicase DDX6 Controls Cellular Plasticity by Modulating P-Body Homeostasis. In Cell stem cell, 25, 622-638.e13. doi:10.1016/j.stem.2019.08.018. https://pubmed.ncbi.nlm.nih.gov/31588046/
2. Ripin, Nina, Macedo de Vasconcelos, Luisa, Ugay, Daniella A, Parker, Roy. 2024. DDX6 modulates P-body and stress granule assembly, composition, and docking. In The Journal of cell biology, 223, . doi:10.1083/jcb.202306022. https://pubmed.ncbi.nlm.nih.gov/38536035/
3. Ostareck, Dirk H, Naarmann-de Vries, Isabel S, Ostareck-Lederer, Antje. 2014. DDX6 and its orthologs as modulators of cellular and viral RNA expression. In Wiley interdisciplinary reviews. RNA, 5, 659-78. doi:10.1002/wrna.1237. https://pubmed.ncbi.nlm.nih.gov/24788243/
4. Wiley, Mandi M, Khatri, Bhuwan, Joachims, Michelle L, Nordmark, Gunnel, Lessard, Christopher J. 2023. Variants in the DDX6-CXCR5 autoimmune disease risk locus influence the regulatory network in immune cells and salivary gland. In bioRxiv : the preprint server for biology, , . doi:10.1101/2023.10.05.561076. https://pubmed.ncbi.nlm.nih.gov/39071447/
5. Naito, Seiichiro, Tanaka, Hiroki, Jiang, Jing-Jing, Hashimoto, Shigeru, Murakami, Masaaki. 2024. DDX6 is involved in the pathogenesis of inflammatory diseases via NF-κB activation. In Biochemical and biophysical research communications, 703, 149666. doi:10.1016/j.bbrc.2024.149666. https://pubmed.ncbi.nlm.nih.gov/38377944/
6. Deng, Xin, Liu, Zhen, Wang, Baosheng, Ma, Jia, Meng, Xiangpeng. . The DDX6/KIFC1 signaling axis, as regulated by YY1, contributes to the malignant behavior of pancreatic cancer. In FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 38, e23581. doi:10.1096/fj.202400166R. https://pubmed.ncbi.nlm.nih.gov/38551642/
7. Shih, Chia-Yu, Chen, Yun-Chi, Lin, Heng-Yi, Chu, Chia-Ying. 2023. RNA Helicase DDX6 Regulates A-to-I Editing and Neuronal Differentiation in Human Cells. In International journal of molecular sciences, 24, . doi:10.3390/ijms24043197. https://pubmed.ncbi.nlm.nih.gov/36834609/
품질 관리 기준
정자 검사
동결 보존 전: 정자 농도 측정 및 정자 생존율 평가.
동결 보존 후: 각 배치에서 동결 보존된 정자 바이알 1개를 선택하여 체외수정(in vitro fertilization)에 사용합니다.
Environmental Standards:
SPFAvailable Region:
GlobalSource:
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