Lep-flox Mouse
Common Name
Lep-flox
제품 ID
S-CKO-03377
Backgroud
C57BL/6JCya
품종 계통계통 ID
CKOCMP-16846-Lep-B6J-VA
상태
이 마우스 계통을 논문에서 사용할 경우, “Lep-flox Mouse (카탈로그 번호 S-CKO-03377)은 Cyagen에서 구입하였습니다.”라고 명시해 주시기 바랍니다.
구매 가능한 제품 종류
연령
Genotype
성별
수량
표준 제공 조건은 최소 3마리의 이형접합(heterozygous) 보균자를 보장합니다. 동형접합(homozygous) 보균자 및/또는 특정 성별에 대한 브리딩 서비스도 제공됩니다.
기본 정보
품종 계통
Lep-flox
품종 계통계통 ID
CKOCMP-16846-Lep-B6J-VA
유전자명
제품 ID
S-CKO-03377
유전자 별칭
ob, obese
배경
C57BL/6JCya
NCBI ID
변형 내용
Conditional knockout
염색체
Chr 6
Phenotype
Datasheet
적용 분야
--
품종 계통 설명
Ensembl 전사체 ID
ENSMUST00000069789
NCBI 전사체 ID
NM_008493
타겟 영역
Exon 3
유효 영역 크기
~3.8 kb
유전자 연구 개요
Lep, which encodes leptin, is a crucial gene. Leptin is a hormone mainly produced by adipose tissue and is a key regulator of energy balance, acting on the hypothalamus to regulate appetite and metabolism. It is also involved in multiple biological processes such as inflammation, fibrosis, and cell growth [1,2,5].
Lep seems to have a dual-edged role in wound healing. It exerts pro-inflammatory and profibrotic effects, but can also regulate hair follicle growth, making it a potential target for promoting scarless wound healing [1].
In breast cancer, LEP is downregulated in cancer tissues compared to normal tissues, and low LEP expression is associated with a poorer prognosis, suggesting it could be a potential biomarker and therapeutic target [2].
Regarding non-Hodgkin lymphoma, the LEP G2528A polymorphism may increase the risk, while the A19G polymorphism may decrease the risk, especially for follicular lymphoma [3]. Some LEP gene variants like rs7799039 may be associated with energy intake [4].
In bovine intramuscular preadipocytes, LEP inhibits adipogenesis through the AMPK signaling pathway [5].
In preeclampsia, miR-519d can downregulate LEP expression to inhibit the development of the disease [6].
In myeloid neoplasms, hypermethylation of the LEP promoter is an early event and is associated with a worse prognosis [7].
In the Ukrainian population, the LEPR gene polymorphism (rs1137101) may be related to the metabolic syndrome, and the presence of its alleles affects the level of leptin and other hormones [8].
In Prader-Willi syndrome, there are alterations in serum leptin and LEP/LEPR promoter methylation [9].
In conclusion, Lep is essential in regulating energy balance, and its dysregulation is associated with various diseases including wound-related fibrotic disorders, breast cancer, non-Hodgkin lymphoma, preeclampsia, myeloid neoplasms, the metabolic syndrome, and Prader-Willi syndrome. Studies on Lep, especially through genetic models, help understand the biological processes and mechanisms underlying these diseases, potentially providing new strategies for diagnosis and treatment.
References：
1. Zhang, Kai-Wen, Jia, Yuan, Li, Yue-Yue, Yuan, Zheng-Dong, Yuan, Feng-Lai. 2022. LEP and LEPR are possibly a double-edged sword for wound healing. In Journal of cellular physiology, 238, 355-365. doi:10.1002/jcp.30936. https://pubmed.ncbi.nlm.nih.gov/36571294/
2. Jin, Tong Yi, Saindane, Madhuri, Park, Kyoung Sik, Yang, Jung-Hyun, Yun, IkJin. . LEP as a potential biomarker in prognosis of breast cancer: Systemic review and meta analyses (PRISMA). In Medicine, 100, e26896. doi:10.1097/MD.0000000000026896. https://pubmed.ncbi.nlm.nih.gov/34414945/
3. Lin, Hai-Yan, Shi, Hui, Li, Chun-Yan, Liu, Peng-Cheng, Lou, Lie-ming. . LEP and LEPR polymorphisms in non-Hodgkin lymphoma risk: a systematic review and pooled analysis. In Journal of B.U.ON. : official journal of the Balkan Union of Oncology, 20, 261-8. doi:. https://pubmed.ncbi.nlm.nih.gov/25778326/
4. Kroll, Caroline, Mastroeni, Silmara S B S, Veugelers, Paul J, Mastroeni, Marco F. 2019. Associations of ADIPOQ and LEP Gene Variants with Energy Intake: A Systematic Review. In Nutrients, 11, . doi:10.3390/nu11040750. https://pubmed.ncbi.nlm.nih.gov/30935050/
5. Yu, Shengchen, Yu, Hengwei, Wang, Jianfang, Mei, Chugang, Zan, Linsen. . LEP inhibits intramuscular adipogenesis through the AMPK signaling pathway in vitro. In FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 38, e23836. doi:10.1096/fj.202400590RR. https://pubmed.ncbi.nlm.nih.gov/39044640/
6. Cai, Hairui, Li, Dongmei, Wu, Jun, Shi, Chunbo. 2021. miR-519d downregulates LEP expression to inhibit preeclampsia development. In Open medicine (Warsaw, Poland), 16, 1215-1227. doi:10.1515/med-2021-0244. https://pubmed.ncbi.nlm.nih.gov/34514168/
7. Kaastrup, Katja, Gillberg, Linn, Mikkelsen, Stine U, Hansen, Jakob W, Grønbæk, Kirsten. 2023. LEP promoter methylation in the initiation and progression of clonal cytopenia of undetermined significance and myelodysplastic syndrome. In Clinical epigenetics, 15, 91. doi:10.1186/s13148-023-01505-w. https://pubmed.ncbi.nlm.nih.gov/37237325/
8. Prodan, Andrii, Dzubanovsky, Ihor, Kamyshnyi, Oleksandr, Pidruchna, Svitlana, Voloshyn, Stanislava. 2023. GHRL, LEP, LEPR genes polymorphism and their association with the metabolic syndrome in the Ukrainian population. In Endocrine regulations, 57, 269-278. doi:10.2478/enr-2023-0030. https://pubmed.ncbi.nlm.nih.gov/38127688/
9. Wieting, Jelte, Jahn, Kirsten, Buchholz, Vanessa, Deest, Maximilian, Frieling, Helge. 2022. Alteration of serum leptin and LEP/LEPR promoter methylation in Prader-Willi syndrome. In Psychoneuroendocrinology, 143, 105857. doi:10.1016/j.psyneuen.2022.105857. https://pubmed.ncbi.nlm.nih.gov/35803048/
품질 관리 기준
정자 검사
동결 보존 전: 정자 농도 측정 및 정자 생존율 평가.
동결 보존 후: 각 배치에서 동결 보존된 정자 바이알 1개를 선택하여 체외수정(in vitro fertilization)에 사용합니다.
Environmental Standards:
SPFAvailable Region:
GlobalSource:
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