Nipal1-flox Mouse
Common Name
Nipal1-flox
제품 ID
S-CKO-14898
Backgroud
C57BL/6JCya
품종 계통계통 ID
CKOCMP-70701-Nipal1-B6J-VA
상태
이 마우스 계통을 논문에서 사용할 경우, “Nipal1-flox Mouse (카탈로그 번호 S-CKO-14898)은 Cyagen에서 구입하였습니다.”라고 명시해 주시기 바랍니다.
구매 가능한 제품 종류
연령
Genotype
성별
수량
표준 제공 조건은 최소 3마리의 이형접합(heterozygous) 보균자를 보장합니다. 동형접합(homozygous) 보균자 및/또는 특정 성별에 대한 브리딩 서비스도 제공됩니다.
기본 정보
품종 계통
Nipal1-flox
품종 계통계통 ID
CKOCMP-70701-Nipal1-B6J-VA
유전자명
제품 ID
S-CKO-14898
유전자 별칭
Npal1, 3830408G10Rik
배경
C57BL/6JCya
NCBI ID
변형 내용
Conditional knockout
염색체
Chr 5
Phenotype
Datasheet
적용 분야
--
품종 계통 설명
Ensembl 전사체 ID
ENSMUST00000087212
NCBI 전사체 ID
NM_001081205
타겟 영역
Exon 4
유효 영역 크기
~1.7 kb
유전자 연구 개요
NIPAL1, encoding a magnesium influx transporter, is a gene with significant biological importance. It is involved in magnesium-related cellular processes and has been associated with multiple biological pathways and diseases [1,2,5]. Genetic models can be valuable in studying its functions.
In pancreatic islets, NIPAL1 expression is regulated by extracellular magnesium. Knockdown of NIPAL1 in Min6-K8 cells (a pancreatic β-cell-like cell line) decreased both basal insulin secretion and total insulin content, while its overexpression increased total insulin content, suggesting its role in glucose-stimulated insulin secretion and intracellular insulin content regulation, especially under hypomagnesemia conditions [1].
In oral squamous cell carcinoma (OSCC), NIPAL1 expression was observed in 20.3% of 192 patients. Its expression correlated with cancer cell intravasation and poorer disease-free survival, and in functional analysis, it regulated the growth and adhesion of OSCC tumor cells and endothelial cells, indicating it might be a novel tumor-promoting factor and a useful molecular marker for OSCC [2].
In a genome-wide association study, NIPAL1 achieved Bonferroni significance in gene-based association tests related to spondylosis [3]. Genome-wide association studies also linked NIPAL1 to gout associated with reduced renal urate excretion, though functional analysis did not detect urate transport via NIPAL1, and its expression in the human kidney was mainly in the distal tubules [4,5,6,7].
In conclusion, NIPAL1 plays essential roles in multiple biological processes and disease conditions. Its function in insulin secretion regulation in pancreatic islets, its potential as a tumor-promoting factor in OSCC, and its associations with spondylosis and gout are significant findings. Studies using various models, including potential KO/CKO mouse models (not explicitly detailed in provided references but inferred as valuable for further study), contribute to understanding these functions and their implications in related diseases.
References：
1. Manialawy, Yousef, Khan, Saifur R, Bhattacharjee, Alpana, Wheeler, Michael B. 2020. The magnesium transporter NIPAL1 is a pancreatic islet-expressed protein that conditionally impacts insulin secretion. In The Journal of biological chemistry, 295, 9879-9892. doi:10.1074/jbc.RA120.013277. https://pubmed.ncbi.nlm.nih.gov/32439805/
2. Sasahira, Tomonori, Nishiguchi, Yukiko, Kurihara-Shimomura, Miyako, Kuniyasu, Hiroki, Kirita, Tadaaki. 2018. NIPA-like domain containing 1 is a novel tumor-promoting factor in oral squamous cell carcinoma. In Journal of cancer research and clinical oncology, 144, 875-882. doi:10.1007/s00432-018-2612-x. https://pubmed.ncbi.nlm.nih.gov/29464350/
3. Zhang, Yanfei, Grant, Ryan A, Shivakumar, Manu K, Williams, Marc S, Lee, Ming Ta Michael. . Genome-wide Association Analysis Across 16,956 Patients Identifies a Novel Genetic Association Between BMP6, NIPAL1, CNGA1 and Spondylosis. In Spine, 46, E625-E631. doi:10.1097/BRS.0000000000003880. https://pubmed.ncbi.nlm.nih.gov/33332786/
4. García-Nieto, Víctor M, Claverie-Martín, Félix, Moraleda-Mesa, Teresa, Luis-Yanes, María I, Ramos-Trujillo, Elena. 2022. Gout associated with reduced renal excretion of uric acid. Renal tubular disorder that nephrologists do not treat. In Nefrologia, 42, 273-279. doi:10.1016/j.nefroe.2022.05.007. https://pubmed.ncbi.nlm.nih.gov/36210617/
5. Nakayama, Akiyoshi, Nakaoka, Hirofumi, Yamamoto, Ken, Merriman, Tony R, Matsuo, Hirotaka. 2016. GWAS of clinically defined gout and subtypes identifies multiple susceptibility loci that include urate transporter genes. In Annals of the rheumatic diseases, 76, 869-877. doi:10.1136/annrheumdis-2016-209632. https://pubmed.ncbi.nlm.nih.gov/27899376/
6. García-Nieto, Víctor M, Claverie-Martín, Félix, Moraleda-Mesa, Teresa, Luis-Yanes, María I, Ramos-Trujillo, Elena. 2021. Gout associated with reduced renal excretion of uric acid. Renal tubular disorder that nephrologists do not treat. In Nefrologia, , . doi:10.1016/j.nefro.2021.03.013. https://pubmed.ncbi.nlm.nih.gov/34503865/
7. Zhu, Weifeng, Deng, Yan, Zhou, Xiaodong. 2018. Multiple Membrane Transporters and Some Immune Regulatory Genes are Major Genetic Factors to Gout. In The open rheumatology journal, 12, 94-113. doi:10.2174/1874312901812010094. https://pubmed.ncbi.nlm.nih.gov/30123371/
품질 관리 기준
정자 검사
동결 보존 전: 정자 농도 측정 및 정자 생존율 평가.
동결 보존 후: 각 배치에서 동결 보존된 정자 바이알 1개를 선택하여 체외수정(in vitro fertilization)에 사용합니다.
Environmental Standards:
SPFAvailable Region:
GlobalSource:
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