Prkaa1-KO Mouse
Common Name
Prkaa1-KO
제품 ID
S-KO-00422
Backgroud
C57BL/6JCya
품종 계통계통 ID
KOCMP-105787-Prkaa1-B6J-VA
상태
이 마우스 계통을 논문에서 사용할 경우, “Prkaa1-KO Mouse (카탈로그 번호 S-KO-00422)은 Cyagen에서 구입하였습니다.”라고 명시해 주시기 바랍니다.
구매 가능한 제품 종류
연령
Genotype
성별
수량
표준 제공 조건은 최소 3마리의 이형접합(heterozygous) 보균자를 보장합니다. 동형접합(homozygous) 보균자 및/또는 특정 성별에 대한 브리딩 서비스도 제공됩니다.
기본 정보
품종 계통
Prkaa1-KO
품종 계통계통 ID
KOCMP-105787-Prkaa1-B6J-VA
유전자명
제품 ID
S-KO-00422
유전자 별칭
AMPKalpha1, C130083N04Rik
배경
C57BL/6JCya
NCBI ID
변형 내용
Conventional knockout
염색체
Chr 15
Phenotype
Datasheet
적용 분야
--
품종 계통 설명
Ensembl 전사체 ID
ENSMUST00000051186
NCBI 전사체 ID
NM_001013367
타겟 영역
Exon 3
유효 영역 크기
~1.3 kb
유전자 연구 개요
Prkaa1, also known as AMPKα1, is the α -subunit of 5 -AMP -activated protein kinase. This kinase is a major cellular sensor of energy and nutrients, playing a key role in regulating cellular energy metabolism through phosphorylation [1,2,7]. It is involved in multiple biological processes and associated with various pathways, such as autophagy, redox balance, and mitophagy, which are crucial for cell survival, development, and homeostasis [5,6,9].
In gene-knockout (KO) mouse models, endothelial-specific Prkaa1 knockout in HFD-fed mice alleviated HFD-induced metabolic syndromes, including decreased body weight, enhanced glucose clearance, and relieved adipose inflammation and hepatic steatosis. Mechanistically, PRKAA1 knockdown in cultured endothelial cells reduced glycolysis, fatty acid oxidation, and suppressed transcription of inflammatory molecules [2]. In Prkaa1-deficient mice, autophagy-dependent mitochondrial clearance during erythrocyte maturation was defective, leading to enhanced oxidative stress, shortened erythrocyte lifespan, and anemia [9].
In conclusion, Prkaa1 is essential for regulating cellular energy metabolism, autophagy, and redox balance. The Prkaa1 KO mouse models have revealed its significant role in metabolic syndrome and erythrocyte maturation. Additionally, studies on Prkaa1 genetic polymorphisms have shown its association with the risk of gastric and esophageal cancers, indicating its importance in cancer-related research [1,3,4,8].
References:
1. Chen, Yongyi, Chen, Siyu, Zhu, Jing, Yu, Qiong, Xu, Songxiao. 2023. PRKAA1 predicts prognosis and is associated with immune characteristics in gastric cancer. In Functional & integrative genomics, 23, 252. doi:10.1007/s10142-023-01176-z. https://pubmed.ncbi.nlm.nih.gov/37482545/
2. Yang, Qiuhua, Ma, Qian, Xu, Jiean, Belin de Chantemèle, Eric J, Huo, Yuqing. 2021. Endothelial AMPKα1/PRKAA1 exacerbates inflammation in HFD-fed mice. In British journal of pharmacology, 179, 1661-1678. doi:10.1111/bph.15742. https://pubmed.ncbi.nlm.nih.gov/34796475/
3. Jiang, You, Li, Wenbo, Lu, Jun, Zhao, Xin, Li, Liang. . Association between PRKAA1 rs13361707 T>C polymorphism and gastric cancer risk: Evidence based on a meta-analysis. In Medicine, 97, e0302. doi:10.1097/MD.0000000000010302. https://pubmed.ncbi.nlm.nih.gov/29620653/
4. Yu, Shuyong, Tu, Ruisha, Chen, Zhaowei, Zhang, Ronglin, Li, Yini. 2023. Association of PTGER4 and PRKAA1 genetic polymorphisms with gastric cancer. In BMC medical genomics, 16, 209. doi:10.1186/s12920-023-01645-1. https://pubmed.ncbi.nlm.nih.gov/37670284/
5. Zhang, Yangmei, Zhou, Xichang, Cheng, Xue, Chen, Kai, Li, Yang. 2022. PRKAA1, stabilized by FTO in an m6A-YTHDF2-dependent manner, promotes cell proliferation and glycolysis of gastric cancer by regulating the redox balance. In Neoplasma, 69, 1338-1348. doi:10.4149/neo_2022_220714N714. https://pubmed.ncbi.nlm.nih.gov/36305690/
6. Tang, Yanling, Zhang, Jingjing, Hu, Zeyu, Xing, Hengrui, Niu, Qiang. 2023. PRKAA1 induces aberrant mitophagy in a PINK1/Parkin-dependent manner, contributing to fluoride-induced developmental neurotoxicity. In Ecotoxicology and environmental safety, 255, 114772. doi:10.1016/j.ecoenv.2023.114772. https://pubmed.ncbi.nlm.nih.gov/36924562/
7. Zhang, Yangmei, Zhou, Xichang, Cheng, Long, Zhang, Youwei, Sun, Sanyuan. 2019. PRKAA1 Promotes Proliferation and Inhibits Apoptosis of Gastric Cancer Cells Through Activating JNK1 and Akt Pathways. In Oncology research, 28, 213-223. doi:10.3727/096504019X15668125347026. https://pubmed.ncbi.nlm.nih.gov/31558185/
8. Li, Cheng-Lin, Zhao, Jian-Qiang, Zang, Bao. 2020. PRKAA1 rs13361707 C/T polymorphism confers decreased susceptibility to esophageal cancer: A case-control study. In Journal of clinical laboratory analysis, 34, e23406. doi:10.1002/jcla.23406. https://pubmed.ncbi.nlm.nih.gov/32488984/
9. Zhu, Huaiping, Foretz, Marc, Xie, Zhonglin, Viollet, Benoit, Zou, Ming-Hui. 2014. PRKAA1/AMPKα1 is required for autophagy-dependent mitochondrial clearance during erythrocyte maturation. In Autophagy, 10, 1522-34. doi:10.4161/auto.29197. https://pubmed.ncbi.nlm.nih.gov/24988326/
품질 관리 기준
정자 검사
동결 보존 전: 정자 농도 측정 및 정자 생존율 평가.
동결 보존 후: 각 배치에서 동결 보존된 정자 바이알 1개를 선택하여 체외수정(in vitro fertilization)에 사용합니다.
Environmental Standards:
SPFAvailable Region:
GlobalSource:
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