Glyat-KO Mouse
Common Name
Glyat-KO
제품 ID
S-KO-00490
Backgroud
C57BL/6JCya
품종 계통계통 ID
KOCMP-107146-Glyat-B6J-VA
상태
이 마우스 계통을 논문에서 사용할 경우, “Glyat-KO Mouse (카탈로그 번호 S-KO-00490)은 Cyagen에서 구입하였습니다.”라고 명시해 주시기 바랍니다.
구매 가능한 제품 종류
연령
Genotype
성별
수량
표준 제공 조건은 최소 3마리의 이형접합(heterozygous) 보균자를 보장합니다. 동형접합(homozygous) 보균자 및/또는 특정 성별에 대한 브리딩 서비스도 제공됩니다.
기본 정보
품종 계통
Glyat-KO
품종 계통계통 ID
KOCMP-107146-Glyat-B6J-VA
유전자명
제품 ID
S-KO-00490
유전자 별칭
CAT, GAT, ACGNAT, A330009E03Rik
배경
C57BL/6JCya
NCBI ID
변형 내용
Conventional knockout
염색체
Chr 19
Phenotype
Datasheet
적용 분야
--
품종 계통 설명
Ensembl 전사체 ID
ENSMUST00000044976
NCBI 전사체 ID
NM_145935.3
타겟 영역
Exon 3~4
유효 영역 크기
~2.8 kb
유전자 연구 개요
GLYAT, or glycine N-acyltransferase, is an enzyme that catalyzes the transfer of acyl groups from acyl CoA to glycine, resulting in acyl glycine and coenzyme A. It is a key component of the phase II glycine conjugation pathway in the liver, playing a role in the detoxification of endogenous and xenobiotic compounds containing a carboxylic acid group, and is important for maintaining adequate levels of free coenzyme A (CoASH) [4,5,6,7].
GLYAT has been found to be lowly expressed in several cancers, including liver cancer, clear cell renal cell carcinoma (ccRCC), and breast cancer. In liver cancer and ccRCC, overexpression of GLYAT inhibits cell proliferation and migration, while interfering with GLYAT expression rescues these abilities. It was also shown to downregulate Rho-associated coiled-coil-containing protein kinase 1 (ROCK1), suggesting that GLYAT suppresses the progression of these cancers by downregulating ROCK1 [1]. In breast cancer, knockdown of GLYAT augmented cell proliferation in vitro and in vivo, and its downregulation promoted cell migration likely through the activation of the PI3K/AKT/Snail signaling pathway, which induced epithelial-mesenchymal transition (EMT) [3].
In conclusion, GLYAT is important in metabolic detoxification pathways. Its downregulation in various cancers has been associated with tumor progression, highlighting its potential as a prognostic biomarker and therapeutic target. The functional studies in cancer cell lines and in vivo models have provided insights into its role in cancer development, suggesting that understanding GLYAT could offer new strategies for cancer treatment [1,2,3,8].
References:
1. Xia, Yechen, Huang, Wentao, Jin, Guang-Zhi. 2024. GLYAT suppresses liver cancer and clear cell renal cell carcinoma progression by downregulating ROCK1 expression. In Translational cancer research, 13, 5097-5111. doi:10.21037/tcr-24-1412. https://pubmed.ncbi.nlm.nih.gov/39430840/
2. Jiang, Fengchen, Zhou, Shuiping, Xia, Chuanlong, Dai, Feng, Fu, Shouzhong. . Downregulation of GLYAT correlates with tumour progression and poor prognosis in hepatocellular carcinoma. In Journal of cellular and molecular medicine, 28, e70197. doi:10.1111/jcmm.70197. https://pubmed.ncbi.nlm.nih.gov/39495775/
3. Tian, Xin, Wu, Lina, Jiang, Min, Liu, Caigang, Gao, Song. 2021. Downregulation of GLYAT Facilitates Tumor Growth and Metastasis and Poor Clinical Outcomes Through the PI3K/AKT/Snail Pathway in Human Breast Cancer. In Frontiers in oncology, 11, 641399. doi:10.3389/fonc.2021.641399. https://pubmed.ncbi.nlm.nih.gov/33968740/
4. Kühn, Stefan, Williams, Monray E, Dercksen, Marli, Sass, Jörn Oliver, van der Sluis, Rencia. 2023. The glycine N-acyltransferases, GLYAT and GLYATL1, contribute to the detoxification of isovaleryl-CoA - an in-silico and in vitro validation. In Computational and structural biotechnology journal, 21, 1236-1248. doi:10.1016/j.csbj.2023.01.041. https://pubmed.ncbi.nlm.nih.gov/36817957/
5. Schulke, Daniel, Sass, Jörn Oliver. 2021. Frequent sequence variants of human glycine N-acyltransferase (GLYAT) and inborn errors of metabolism. In Biochimie, 183, 30-34. doi:10.1016/j.biochi.2021.02.002. https://pubmed.ncbi.nlm.nih.gov/33567294/
6. Lino Cardenas, Christian Lacks, Bourgine, Joanna, Cauffiez, Christelle, Broly, Frank, Chevalier, Dany. 2010. Genetic polymorphisms of glycine N-acyltransferase (GLYAT) in a French Caucasian population. In Xenobiotica; the fate of foreign compounds in biological systems, 40, 853-61. doi:10.3109/00498254.2010.519407. https://pubmed.ncbi.nlm.nih.gov/20925583/
7. Badenhorst, Christoffel Petrus Stephanus, van der Sluis, Rencia, Erasmus, Elardus, van Dijk, Alberdina Aike. 2013. Glycine conjugation: importance in metabolism, the role of glycine N-acyltransferase, and factors that influence interindividual variation. In Expert opinion on drug metabolism & toxicology, 9, 1139-53. doi:10.1517/17425255.2013.796929. https://pubmed.ncbi.nlm.nih.gov/23650932/
8. Muñoz, Juan P, Calaf, Gloria M. 2023. Downregulation of Glycine N-Acyltransferase in Kidney Renal Clear Cell Carcinoma: A Bioinformatic-Based Screening. In Diagnostics (Basel, Switzerland), 13, . doi:10.3390/diagnostics13233505. https://pubmed.ncbi.nlm.nih.gov/38066746/
품질 관리 기준
정자 검사
동결 보존 전: 정자 농도 측정 및 정자 생존율 평가.
동결 보존 후: 각 배치에서 동결 보존된 정자 바이알 1개를 선택하여 체외수정(in vitro fertilization)에 사용합니다.
Environmental Standards:
SPFAvailable Region:
GlobalSource:
Cyagen문의하기
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