Dhrs4-KO Mouse
Common Name
Dhrs4-KO
제품 ID
S-KO-17183
Backgroud
C57BL/6JCya
품종 계통계통 ID
KOCMP-28200-Dhrs4-B6J-VB
상태
이 마우스 계통을 논문에서 사용할 경우, “Dhrs4-KO Mouse (카탈로그 번호 S-KO-17183)은 Cyagen에서 구입하였습니다.”라고 명시해 주시기 바랍니다.
구매 가능한 제품 종류
연령
Genotype
성별
수량
표준 제공 조건은 최소 3마리의 이형접합(heterozygous) 보균자를 보장합니다. 동형접합(homozygous) 보균자 및/또는 특정 성별에 대한 브리딩 서비스도 제공됩니다.
기본 정보
품종 계통
Dhrs4-KO
품종 계통계통 ID
KOCMP-28200-Dhrs4-B6J-VB
유전자명
제품 ID
S-KO-17183
유전자 별칭
CR, RRD, NDRD, PHCR, PSCD, mouNRDR, D14Ucla2
배경
C57BL/6JCya
NCBI ID
변형 내용
Conventional knockout
염색체
Chr 14
Phenotype
Datasheet
적용 분야
--
품종 계통 설명
Ensembl 전사체 ID
ENSMUST00000022821
NCBI 전사체 ID
NM_001037938
타겟 영역
Exon 2
유효 영역 크기
~1.0 kb
유전자 연구 개요
DHRS4, also known as dehydrogenase/reductase 4, encodes a NADP(H)-dependent retinol dehydrogenase/reductase enzyme [9]. It is part of a gene cluster including DHRS4L2 and DHRS4L1, and the genes in this cluster may be transcriptionally regulated through antisense transcription mechanisms [9]. DHRS4 enzymes share common sequence motives related to coenzyme binding and catalytic mechanism with its paralogue DHRS2, but have different substrate-binding sequences [8].
In various diseases, DHRS4-AS1, an antisense long non-coding RNA of DHRS4, has shown significant effects. In gastric cancer, it inhibits cell proliferation and promotes apoptosis by destabilizing pro-oncogenic DHX9 and inhibiting the association between DHX9 and ILF3 [1]. In hepatocellular carcinoma, it suppresses proliferation and promotes apoptosis via the miR-522-3p/SOCS5 axis [2]. In NSCLC, it inhibits the stemness of cancer cells by sponging miR-224-3p and upregulating TP53 and TET1 [3]. In endometriosis, it inhibits ectopic endometrial cell proliferation, migration, and invasion by regulating miR-139-5p expression [5]. In thyroid cancer, it may act as a diagnostic factor by sponging miR-222-3p and regulating cancer cell proliferation and apoptosis [6]. In ulcerative colitis, METTL14-regulated DHRS4-AS1 mitigates inflammatory injury via the miR-206/A3AR axis [4]. In gliomas, miR-29a-5p suppresses cell proliferation, invasion, and migration by targeting DHRS4, suggesting DHRS4 may be an oncogene [7].
In conclusion, studies related to DHRS4 and its associated non-coding RNA DHRS4-AS1 have revealed their important roles in multiple disease processes such as cancer and inflammatory diseases. These findings help in understanding the molecular mechanisms underlying these diseases and may potentially provide new therapeutic targets and diagnostic biomarkers.
References:
1. Xiao, Lei, Zhang, Yang, Luo, Qingqing, Chen, Zihua, Lai, Chen. 2023. DHRS4-AS1 regulate gastric cancer apoptosis and cell proliferation by destabilizing DHX9 and inhibited the association between DHX9 and ILF3. In Cancer cell international, 23, 304. doi:10.1186/s12935-023-03151-x. https://pubmed.ncbi.nlm.nih.gov/38041141/
2. Zhou, Yongping, Li, Kuan, Zou, Xuexia, Chen, Fangming, Dai, Tu. . LncRNA DHRS4-AS1 ameliorates hepatocellular carcinoma by suppressing proliferation and promoting apoptosis via miR-522-3p/SOCS5 axis. In Bioengineered, 12, 10862-10877. doi:10.1080/21655979.2021.1994719. https://pubmed.ncbi.nlm.nih.gov/34666613/
3. Yan, Fei, Zhao, Wei, Xu, Xiaoyue, Shi, Lin, Wu, Yuan. 2020. LncRNA DHRS4-AS1 Inhibits the Stemness of NSCLC Cells by Sponging miR-224-3p and Upregulating TP53 and TET1. In Frontiers in cell and developmental biology, 8, 585251. doi:10.3389/fcell.2020.585251. https://pubmed.ncbi.nlm.nih.gov/33425890/
4. Wu, Weiyun, Li, Xiaowen, Zhou, Zhuliang, Ye, Shicai, Quan, Juan-Hua. 2024. METTL14 regulates inflammation in ulcerative colitis via the lncRNA DHRS4-AS1/miR-206/A3AR axis. In Cell biology and toxicology, 40, 95. doi:10.1007/s10565-024-09944-8. https://pubmed.ncbi.nlm.nih.gov/39528760/
5. Cui, Xuan, Zhou, Shisan, Lin, Yongtao. . Long non-coding RNA DHRS4 antisense RNA 1 inhibits ectopic endometrial cell proliferation, migration, and invasion in endometriosis by regulating microRNA-139-5p expression. In Bioengineered, 13, 9792-9804. doi:10.1080/21655979.2022.2060781. https://pubmed.ncbi.nlm.nih.gov/35414313/
6. Xu, Shuai, Zheng, Xinyi, Wu, Haibin, Liu, Qianqian, Ye, Ren-Qun. 2024. Feasibility study of lncRNA DHRS4-AS1 sponge miR-222-3p in the diagnosis of thyroid cancer. In Endokrynologia Polska, 75, 494-500. doi:10.5603/ep.99456. https://pubmed.ncbi.nlm.nih.gov/39376175/
7. Dai, Yong, Chen, Zhenhua, Zhao, Wei, Hu, Hongkang, Zhang, Yi. 2020. miR-29a-5p Regulates the Proliferation, Invasion, and Migration of Gliomas by Targeting DHRS4. In Frontiers in oncology, 10, 1772. doi:10.3389/fonc.2020.01772. https://pubmed.ncbi.nlm.nih.gov/33014873/
8. Gabrielli, Franco, Tofanelli, Sergio. 2012. Molecular and functional evolution of human DHRS2 and DHRS4 duplicated genes. In Gene, 511, 461-9. doi:10.1016/j.gene.2012.09.013. https://pubmed.ncbi.nlm.nih.gov/23036705/
9. Su, Zhong-Jing, Zhang, Qiao-Xia, Liu, Ge-Fei, Sui, Xu-Xia, Huang, Dong-Yang. 2010. Bioinformatic analysis of the human DHRS4 gene cluster and a proposed mechanism for its transcriptional regulation. In BMC molecular biology, 11, 43. doi:10.1186/1471-2199-11-43. https://pubmed.ncbi.nlm.nih.gov/20525226/
품질 관리 기준
정자 검사
동결 보존 전: 정자 농도 측정 및 정자 생존율 평가.
동결 보존 후: 각 배치에서 동결 보존된 정자 바이알 1개를 선택하여 체외수정(in vitro fertilization)에 사용합니다.
Environmental Standards:
SPFAvailable Region:
GlobalSource:
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