The CX3C chemokine receptor 1 (CX3CR1) gene encodes a G protein-coupled receptor that primarily functions to recognize and bind the CX3CL1 chemokine protein. The CX3CR1-CX3CL1 signaling pathway plays a crucial role in regulating immune cell migration during inflammatory responses, neuronal migration, and synapse formation, among other physiological and pathological processes. The dysregulation of CX3CR1 has been implicated in various diseases, including inflammatory diseases, cancer, and neurological disorders. As such, the CX3CR1-CX3CL1 signaling pathway is widely studied to understand the biological processes of the immune and nervous systems and the pathogenesis of related diseases. CX3CR1 is predominantly expressed in the immune and nervous systems, particularly on the surface of mononuclear macrophages and microglia. It is also expressed in monocytes, circulating T cell subsets, natural killer cells, and dendritic cells.^[1] Cx3cr1-iCre mice are generated using gene editing technology to replace the coding sequence (CDS) region of the endogenous mouse Cx3cr1 gene with an improved Cre recombinase (iCre) expression element. This results in an expression pattern of Cre recombinase that is similar to that of the endogenous gene. When these mice are crossed with mice containing a loxP site-flanked sequence, Cre recombinase-mediated recombination leads to deletion of the flanked sequence in brain microglia and macrophages in other parts of the offspring mice.

1700013G24Rik is located on chromosome 4 of mice. Nuclease Technology will be used to design sgRNA; 1700013G24Rik knockout mice will be obtained by applying high-throughput electroporation of fertilized eggs. After sexual maturity, sperm were collected for cryopreservation.

Srp19 is located on chromosome 18 of mice. SgRNA and ssDNA will be designed using Nuclease Technology; Srp19 conditional knockout mice will be obtained by high-throughput electroporation of fertilized eggs. After sexual maturity, sperm will be collected for cryopreservation.

Cx3cr1 is located on chromosome 9 of mice. Nuclease Technology was used to design sgRNA; Cx3cr1 knockout mice were obtained by applying high-throughput electroporation of fertilized eggs. After sexual maturity, sperm were collected for cryopreservation.

Cx3cr1 is located on chromosome 9 of mice. SgRNA and ssDNA were designed using Nuclease Technology; Cx3cr1 conditional knockout mice were obtained by high-throughput electroporation of fertilized eggs. After sexual maturity, sperm were collected for cryopreservation.