Foxj1 (forkhead-box J1), also known as hepatocyte nuclear factor-3 (HNF-3) or forkhead homologue-4 (HFH-4), is a member of the forkhead-box transcription factor family, a class of transcription factors with a winged helix structure that binds to DNA. The Foxj1 factor plays a major regulatory role in controlling the transcription process of motile cilia synthesis. Airway cilia quickly clear airway mucus by swinging, playing a role in resisting pathogen invasion. It also has an effect on the left-right axis asymmetry of organ development during the embryonic period. This strain is a Foxj1 gene knockout mouse model. Studies have shown that Foxj1 KO mice exhibit left-right asymmetric development and airway ciliated epithelium is lost in adult mice ^[1]. The heterozygous Foxj1 KO mice are viable, while the homozygous mice are lethal.

Vsig1 is located on chromosome X of mice. Nuclease Technology was used to design sgRNA; Vsig1 knockout mice were obtained by applying high-throughput electroporation of fertilized eggs. After sexual maturity, sperm were collected for cryopreservation.

Foxj1 is located on chromosome 11 of mice. Nuclease Technology will be used to design sgRNA; Foxj1 knockout mice will be obtained by applying high-throughput electroporation of fertilized eggs. After sexual maturity, sperm were collected for cryopreservation.

Wfdc3 is located on chromosome 2 of mice. SgRNA and ssDNA will be designed using Nuclease Technology; Wfdc3 conditional knockout mice will be obtained by high-throughput electroporation of fertilized eggs. After sexual maturity, sperm will be collected for cryopreservation.

Foxj1 is located on chromosome 11 of mice. SgRNA and ssDNA will be designed using Nuclease Technology; Foxj1 conditional knockout mice will be obtained by high-throughput electroporation of fertilized eggs. After sexual maturity, sperm will be collected for cryopreservation.