The ALB gene encodes albumin, mainly produced in the liver, and is the most abundant protein in human plasma, accounting for 60% to 65% of total plasma protein. The proprotein encoded by ALB is processed to produce a functional protein, and the EPI-X4 peptide derived from this protein is an endogenous inhibitor of the CXCR4 chemokine receptor. Albumin plays a role in regulating plasma colloid osmotic pressure, helping to maintain blood circulation and isolating and transporting many metabolites within the body, especially insoluble hydrophobic metabolites ^[1]. Human Serum Albumin (HSA) is an important carrier protein involved in the transport of a variety of endogenous molecules, including hormones, fatty acids, and metabolic products, as well as exogenous drugs. As a natural carrier protein, HSA has multiple ligand binding sites and a plasma half-life of up to 19 days, making it a promising drug carrier. Several HSA-based drug delivery systems have been approved for clinical trials ^[2-3]. In addition, albumin is also the main transporter of zinc, calcium, and magnesium in plasma, binding approximately 80% of all plasma zinc and approximately 45% of circulating calcium and magnesium, with an affinity ranking of zinc > calcium > magnesium ^[4]. Diseases associated with the ALB gene include hyperthyroxinemia, familial serum albumin abnormality, and analbuminemia ^[5]. This strain is a humanized mouse model of the Alb gene, obtained by in situ replacement of the entire mouse Alb gene sequence, including the UTR region, with the human ALB gene sequence. This model can be used for the development of ALB-targeted drugs, as well as for the research and development of drugs using HSA as a carrier, and for in vivo pharmacodynamics and pharmacokinetics studies, including albumin-drug conjugates or albumin-binding prodrugs.

Nlrp6 is located on chromosome 7 of mice. Nuclease Technology was used to design sgRNA; Nlrp6 knockout mice were obtained by applying high-throughput electroporation of fertilized eggs. After sexual maturity, sperm were collected for cryopreservation.

Slco2b1 is located on chromosome 7 of mice. SgRNA and ssDNA will be designed using Nuclease Technology; Slco2b1 conditional knockout mice will be obtained by high-throughput electroporation of fertilized eggs. After sexual maturity, sperm will be collected for cryopreservation.

Rnf213 is located on chromosome 11 of mice. Nuclease Technology was used to design sgRNA; Rnf213 knockout mice were obtained by applying high-throughput electroporation of fertilized eggs. After sexual maturity, sperm were collected for cryopreservation.

Rnf213 is located on chromosome 11 of mice. Nuclease Technology was used to design sgRNA; Rnf213 knockout mice were obtained by applying high-throughput electroporation of fertilized eggs. After sexual maturity, sperm were collected for cryopreservation.

Rnf213 is located on chromosome 11 of mice. SgRNA and ssDNA were designed using Nuclease Technology; Rnf213 conditional knockout mice were obtained by high-throughput electroporation of fertilized eggs. After sexual maturity, sperm were collected for cryopreservation.

Zfp213 is located on chromosome 17 of mice. Nuclease Technology will be used to design sgRNA; Zfp213 knockout mice will be obtained by applying high-throughput electroporation of fertilized eggs. After sexual maturity, sperm were collected for cryopreservation.

Zfp213 is located on chromosome 17 of mice. SgRNA and ssDNA will be designed using Nuclease Technology; Zfp213 conditional knockout mice will be obtained by high-throughput electroporation of fertilized eggs. After sexual maturity, sperm will be collected for cryopreservation.

Or6c213 is located on chromosome 10 of mice. Nuclease Technology will be used to design sgRNA; Or6c213 knockout mice will be obtained by applying high-throughput electroporation of fertilized eggs. After sexual maturity, sperm were collected for cryopreservation.

Tmem213 is located on chromosome 6 of mice. SgRNA and ssDNA will be designed using Nuclease Technology; Tmem213 conditional knockout mice will be obtained by high-throughput electroporation of fertilized eggs. After sexual maturity, sperm will be collected for cryopreservation.