Trp53-KO Mouse

Transcription levels of mouse Trp53 in liver and thymus tissues were determined by RT‑qPCR. Mouse Trp53 transcripts were detected using specific primers in liver and thymus tissues of male Trp53‑KO and WT mice, with mGapdh as the internal reference gene. Relative expression levels were calculated for each tissue, and data are presented as mean ± SD. Results showed that mouse Trp53 transcripts were undetectable in multiple tissues of Trp53‑KO mice. In contrast, mouse Trp53 transcripts were detected in multiple tissues of WT mice. These results indicate that Trp53‑KO mice do not express Trp53 transcripts, confirming the successful generation of the knockout model.