Name: H11-Col2a1-iCre Mouse
Brand: 싸이아젠(Cyagen)
SKU: C001355

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H11-Col2a1-iCre Mouse

제품명

H11-Col2a1-iCre Mouse

제품 ID

C001355

품종 계통

C57BL/6JCya-Igs2^{em1(Col2a1-IRES-iCre)}/Cya

Backgroud

C57BL/6JCya

Examples of Expressing Tissues/Cells

Chondrocytes.

상태

이 마우스 계통을 논문에서 사용할 경우, “H11-Col2a1-iCre Mouse (카탈로그 번호 C001355)은 Cyagen에서 구입하였습니다.”라고 명시해 주시기 바랍니다.

Cre Mouse Models

Safe Harbor Knock-in

구매 가능한 제품 종류

연령

Genotype

성별

수량

가격 문의

Cre Mouse Models

Safe Harbor Knock-in

기본 정보

검증 데이터

This strain was generated by integrating the mouse Col2a1 gene promoter-regulated codon-improved Cre recombinase (iCre) gene expression cassette into the mouse genome. This results in the conditional expression of iCre in chondrocytes, under the control of the Col2a1 promoter. When bred with mice containing a loxP site-flanked sequence, Cre recombinase-mediated deletion of the flanked sequence will occur specifically in chondrocytes in the offspring. Both homozygous and heterozygous H11-Col2a1-iCre mice are viable and fertile.

변형 전략

The mouse Col2a1 gene mouse Col2a1 promoter-driven iCre gene expression element was inserted into the H11 safety harbor.

Figure 1. Diagram of the gene editing strategy for the generation of H11-Col2a1-iCre mice.

검증 데이터

1. Method

H11-Col2a1-iCre mice were crossed with Rosa26-LSL-tdTomato mice, which conditionally express tdTomato, to generate double-heterozygous offspring. In the offspring, Cre recombinase-mediated deletion of the expression termination element (LSL) resulted in tdTomato protein expression in Cre-positive cells. Ankle, knee, and elbow joint tissues from 7-week-old offspring mice were collected and examined for tdTomato protein expression by frozen sections and immunohistochemistry to determine Cre recombinase activity.

2. Group

Cre+ : H11-Col2a1-iCre[KI/+];Rosa26-LSL-tdTomato[CKI/+]

Cre- : Rosa26-LSL-tdTomato[CKI/CKI]

3. Result

（1）Expression of Cre recombinase in ankle joints

Abundant orange spontaneous fluorescence signals were observed in the ankle joints of Cre+ mice, indicating strong Cre recombinase activity in the ankle joints of this model. No tdTomato protein signals were detected in the control group (Cre-), suggesting no expression of Cre recombinase.

Figure 2. Expression of Cre recombinase in the ankle joint.

（2）Expression of Cre recombinase in elbow joints

Abundant orange spontaneous fluorescence signals were observed in the elbow joints of Cre+ mice, indicating strong Cre recombinase activity in the elbow joints of this model. No tdTomato protein signals were detected in the control group (Cre-), suggesting no expression of Cre recombinase.

Figure 3. Expression of Cre recombinase in the elbow joint.

（3）Immunohistochemistry of knee joints

Results showed that corresponding cells in the knee joints of Cre+ mice exhibited strong tdTomato-positive signals.

Figure 4. Immunohistochemical detection of tdTomato protein expression in the knee joint.

4. Summary

In H11-Col2a1-iCre mice, Cre recombinase is primarily expressed in chondrocytes in joints such as the ankle, knee, and elbow. Internal data show that Cre recombinase is also expressed in small amounts in other tissues such as the heart, lungs, tail, brain, brown fat, and sternum (not shown in this manual). In conclusion, this model is a specific Cre mouse that primarily targets chondrocytes, but it is important to note that leakage to other tissues may potentially affect the actual results when using this model.

H11-Col2a1-iCre Mouse

기본 정보

검증 데이터

관련 자료

유전자명

유전자 별칭

NCBI ID

염색체

MGI ID

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Datasheet

품종 계통 설명

변형 전략

1. Method

2. Group

3. Result

4. Summary

관련 제품

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