Name: Cdh16-MerCreMer Mouse
Brand: 싸이아젠(Cyagen)
SKU: C001432

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Cdh16-MerCreMer Mouse

제품명

Cdh16-MerCreMer Mouse

제품 ID

C001432

품종 계통

C57BL/6JCya-Cdh16^{em1(IRES-MerCreMer)}/Cya

Backgroud

C57BL/6JCya

상태

이 마우스 계통을 논문에서 사용할 경우, “Cdh16-MerCreMer Mouse (카탈로그 번호 C001432)은 Cyagen에서 구입하였습니다.”라고 명시해 주시기 바랍니다.

Inducible Cre Mouse Models

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Inducible Cre Mouse Models

기본 정보

검증 데이터

Cdh16-MerCreMer mice were generated by inserting a tamoxifen-inducible Cre recombinase protein (MerCreMer) expression element downstream of the stop codon of the mouse Cdh16 gene. The expression pattern of MerCreMer mirrored that of the endogenous gene. Before induction, MerCreMer was localized to the cytoplasm, and only after tamoxifen treatment could it translocate to the nucleus to exert its recombination effect. When bred with mice containing a loxP site-flanked sequence, Cre recombinase-mediated deletion of the flanked sequence will occur in the renal tubular epithelial cells of the offspring following tamoxifen induction.

변형 전략

The "IRES-MerCreMer" gene expression element was inserted into the 3'UTR of the mouse Cdh16 gene using gene editing technology.

Figure 1. Diagram of the gene editing strategy for the generation of Cdh16-MerCreMer mice.

검증 데이터

1. Method

Sperm from Cdh16-MerCreMer mice were used to fertilize oocytes from Rosa26-LSL-tdTomato mice, generating double-heterozygous offspring. At 6 weeks of age, these offspring were subjected to Tamoxifen induction via gavage at a dose of 4 mg per mouse for 5 days. This resulted in Cre recombinase-mediated deletion of LSL elements and subsequent expression of tdTomato in Cre-positive cells. One week after induction, lung, and renal tissues were collected from the offspring. The distribution of tdTomato protein was determined by immunofluorescence staining and immunohistochemistry to assess the expression of Cre recombinase.

2. Groups

Cre+Tam+: Cdh16-MerCreMer [KI/+]; Rosa26-LSL-tdTomato [CKI/+], with Tamoxifen;

Cre+Tam-: Cdh16-MerCreMer [KI/+]; Rosa26-LSL-tdTomato [CKI/+], without Tamoxifen.

3. Result

（1）Immunohistochemistry of renal tissue

The presence of tdTomato-positive signals in renal tubular epithelial cells of tamoxifen-induced Cdh16-MerCreMer[KI/+];Rosa26-LSL-tdTomato[CKI/+] mice indicates strong expression of Cre recombinase in renal tissues. In contrast, no tdTomato expression or Cre recombinase activity was observed in these tissues in the non-tamoxifen-induced group.

Figure 2. Results of immunohistochemistry of renal tissue.

（2）Immunofluorescence staining of renal tissue

The presence of a large amount of tdTomato-positive signal in the renal tubular epithelial cells of tamoxifen-induced Cdh16-MerCreMer [KI/+];Rosa26-LSL-tdTomato[CKI/+] mice indicates strong Cre recombinase activity at this site. However, a trace amount of tdTomato expression was also observed in the uninduced group, suggesting that this model exhibits a small amount of production leakage in renal tubular epithelial cells.

Figure 3. Results of Immunofluorescence staining of renal tissue.

（3）Immunofluorescence staining of lung tissue

The presence of tdTomato-positive signals in some lung cells of tamoxifen-induced Cdh16-MerCreMer[KI/+];Rosa26-LSL-tdTomato[CKI/+] mice indicates the presence of a small amount of Cre recombinase activity at this site. In contrast, no tdTomato-positive signals or Cre recombinase expression were observed in the uninduced group.

Figure 4. Results of Immunofluorescence staining of lung tissue.

4. Summary

In Cdh16-MerCreMer mice, Cre recombinase expression is primarily localized to renal tubular epithelial cells, with some activity also observed in lung tissue cells. It should be noted that a small amount of Cre recombinase expression leakage occurs in renal tubular epithelial cells before induction. This model represents a highly tamoxifen-inducible, tissue-specific Cre mouse line targeting renal tubular epithelial cells.

Cdh16-MerCreMer Mouse

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변형 전략

1. Method

2. Groups

3. Result

4. Summary

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