Lyz2-IRES-CreERT2 Mouse
제품명
Lyz2-IRES-CreERT2 Mouse
제품 ID
C001499
품종 계통
C57BL/6JCya-Lyz2em3(IRES-CreERT2)/Cya
Backgroud
C57BL/6JCya
Examples of Expressing Tissues/Cells
Bone marrow cell lineages (monocytes, mature macrophages, and granulocytes)
상태
이 마우스 계통을 논문에서 사용할 경우, “Lyz2-IRES-CreERT2 Mouse (카탈로그 번호 C001499)은 Cyagen에서 구입하였습니다.”라고 명시해 주시기 바랍니다.
구매 가능한 제품 종류
연령
Genotype
성별
수량
유전자명
유전자 별칭
Lys, Lzm, Lzp, Lysm, Lyzs, Lyzf2, Lzm-s1
NCBI ID
염색체
Chr 10
MGI ID
Datasheet
품종 계통 설명
Lysozyme, encoded by the LYZ gene, is an integral component of the innate immune system. Its primary function is to disrupt bacterial cell walls by cleaving the β-1,4-glycosidic bond between N-acetylmuramic acid and N-acetylglucosamine in bacterial peptidoglycan, leading to bacterial lysis. Lysozyme is abundant in various secretions, including tears, saliva, human milk, and mucus, as well as in the cytoplasmic granules of macrophages and polymorphonuclear neutrophils. Cytoplasmic lysozyme expression is high in bone marrow cells, Paneth cells, and a subset of salivary gland cells [1-2].
Mice have two lysozyme-encoding genes, Lyz1 and Lyz2, which are highly homologous to the human LYZ gene. Lyz1 is primarily expressed in Paneth cells, while Lyz2 is mainly expressed in myeloid cells. Myeloid-specific expression of Lyz2 is associated with cell-type-specific demethylation of its downstream enhancer. The Lyz2 protein also has lysozyme activity and participates in defense responses against both Gram-negative and Gram-positive bacteria. Lyz2 is expressed in the hematopoietic system, liver, mesothelium, small intestine, and yolk sac of mice [3-4].
Lyz2-IRES-CreERT2 mice were generated using gene editing technology to integrate the tamoxifen-inducible CreERT2 recombinase expression element into the 3’UTR of the mouse Lyz2 gene. This mimics the expression pattern of the endogenous gene while maintaining Lyz2 expression. When bred with mice carrying a loxP site-flanked sequence, Cre recombinase-mediated recombination of the flanked sequence occurs in myeloid cells, including monocytes, mature macrophages, and granulocytes, following tamoxifen induction.
Reference
Yoshimura K, Toibana A, Nakahama K. Human lysozyme: sequencing of a cDNA, and expression and secretion by Saccharomyces cerevisiae. Biochem Biophys Res Commun. 1988 Jan 29;150(2):794-801.
Sharon N. The chemical structure of lysozyme substrates and their cleavage by the enzyme. Proc R Soc Lond B Biol Sci. 1967 Apr 18;167(1009):402-15.
Peters CW, Kruse U, Pollwein R, Grzeschik KH, Sippel AE. The human lysozyme gene. Sequence organization and chromosomal localization. Eur J Biochem. 1989 Jul 1;182(3):507-16.
Clausen BE, Burkhardt C, Reith W, Renkawitz R, Förster I. Conditional gene targeting in macrophages and granulocytes using LysMcre mice. Transgenic Res. 1999 Aug;8(4):265-77.
변형 전략
The IRES-CreERT2 gene expression element was integrated into the 3'UTR region of the mouse Lyz2 gene using gene editing technology.
Figure 1. Diagram of the gene editing strategy for the generation of Lyz2-IRES-CreERT2 mice.
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