Name: Tnni3-Cre Mouse
Brand: 싸이아젠(Cyagen)
SKU: C001562

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Tnni3-Cre Mouse

제품명

Tnni3-Cre Mouse

제품 ID

C001562

품종 계통

C57BL/6JCya-Tnni3^em1(Cre)/Cya

Backgroud

C57BL/6JCya

Examples of Expressing Tissues/Cells

Cardiomyocytes

상태

이 마우스 계통을 논문에서 사용할 경우, “Tnni3-Cre Mouse (카탈로그 번호 C001562)은 Cyagen에서 구입하였습니다.”라고 명시해 주시기 바랍니다.

Cre Mouse Models

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Cre Mouse Models

기본 정보

검증 데이터

Tnni3-Cre mice were constructed by integrating the Cre-rBG pA gene expression component into the mouse Tnni3 gene by gene editing, a strategy that disrupts the expression of the endogenous Tnni3 gene in mice. When Tnni3-Cre mice are crossed with mice containing loxP sites, sequence recombination mediated by Cre recombinase occurs in the offspring's cardiomyocytes.

Reference

Bhavsar PK, Brand NJ, Yacoub MH, Barton PJ. Isolation and characterization of the human cardiac troponin I gene (TNNI3). Genomics. 1996 Jul 1;35(1):11-23.

변형 전략

The Cre-rBG pA cassette was inserted upstream of the ATG start codon in the mouse Tnni3 gene, disrupting the expression of the endogenous Tnni3 gene.

Figure 1. Diagram of the gene editing strategy for the generation of Tnni3-Cre mice.

검증 데이터

1. Method

Tnni3-Cre mice were crossed with Rosa26-LSL-tdTomato mice, which conditionally express a red fluorescent protein (tdTomato). Cre recombinase-mediated deletion of the stop element (LSL) will result in tdTomato protein expression in Cre-positive cells. At 10 weeks of age, collect cardiac tissue from the offspring, and observe tissue autofluorescence using frozen section staining. Collect lung, pancreas, spinal cord, brain, ovary, and testis tissues from the offspring, and analyze tdTomato protein expression using immunofluorescence (IF) staining to determine Cre recombinase expression.

2. Group

Cre+: Tnni3-Cre[KI/+];Rosa26-LSL-tdTomato[CKI/+];

Cre-: Rosa26-LSL-tdTomato[CKI/+].

3. Result

（1）Expression of Cre recombinase in the heart

Histological morphological analysis shows a large amount of orange autofluorescence signal observed in the cardiomyocytes of Cre+ mice, indicating Cre recombination in these cells. No significant fluorescence signal was detected in the corresponding tissue of the control group (Cre-) mice.

Figure 2. Autofluorescence of tdTomato protein in cardiac tissue.

（2）Expression of Cre recombinase in the lung, pancreas, and spinal cord

Results show partial red fluorescence signals in the lungs, pancreas, and spinal cord of Cre+ mice (with a non-positive red background signal), indicating Cre recombination in these tissues.

Figure 3. Immunofluorescence (IF) detection of tdTomato protein in lung, pancreas, and spinal cord.

（3）Expression of Cre recombinase in ovary and testis

Histological morphological analysis shows partial red fluorescence signals in the luteal cells and ovarian stroma of Cre+ mice, indicating Cre recombination in these tissues. No significant fluorescence signal was observed in the testes, indicating no Cre recombination in these tissues.

Figure 4. Immunofluorescence (IF) detection of tdTomato protein in ovaries and testes.

4. Summary

In the Tnni3-Cre mouse model, Cre recombinase is significantly expressed in the cardiomyocytes of adult mice. Additionally, fluorescent recombination signals are detected in the lungs, pancreas, spinal cord, and ovaries (luteal cells, ovarian stroma cells) of the offspring, while no fluorescent recombination signals are observed in the testes.

Tnni3-Cre Mouse

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Reference

변형 전략

1. Method

2. Group

3. Result

4. Summary

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