huCCL1(BALB/c) Mouse

C-C motif chemokine ligand 1 (CCL1), also known as I-309, is a small chemokine mainly secreted by activated T cells, monocytes, and endothelial cells. It belongs to the CC chemokine family and plays a key regulatory role in Th2-type immune responses and the recruitment of regulatory T cells (Tregs) ^[1]. CCL1 binds to its specific receptor CCR8 (C-C chemokine receptor type 8) to mediate the directed migration of Th2 cells and Tregs to inflammatory sites and the tumor microenvironment, promoting the formation of an immunosuppressive tumor microenvironment (TME). It is also involved in the immune regulation of allergic inflammatory responses, autoimmune diseases, and graft-versus-host disease (GvHD) ^[2-3]. The CCL1 gene is located on human chromosome 17 (17q12) and is closely associated with immune evasion in various solid tumors, Th2-type allergic diseases, and the development of inflammatory bowel disease (IBD). Targeting the CCL1-CCR8 signaling axis has become an important strategy for drug development in tumor immunotherapy, allergic diseases, and autoimmune disorders ^[4-5].

The huCCL1(BALB/c) mouse is a humanized model constructed through gene editing technology. The sequences from the start codon to the stop codon of the endogenous mouse Ccl1 gene were replaced with the sequences from the start codon to the stop codon of the human CCL1 gene. This model is suitable for evaluating the efficacy and safety of CCL1-targeted antibodies and CAR-T/CAR-NK cell therapies, as well as for preclinical research in tumor immunity, Th2/Treg immune regulation, allergic disorders, and autoimmune diseases.