Hyou1-flox Mouse

HYOU1, also known as Orp150, is an endoplasmic reticulum molecular chaperone protein. Various stimuli can trigger an unfolded protein response to endoplasmic reticulum stress, accompanied by the up-regulation of HYOU1 expression [2]. HYOU1 is involved in multiple biological processes and associated with many diseases.

In the context of intestinal injury, Fut2-dependent fucosylation of HYOU1 at the N-glycosylation site of asparagine (Asn) 862 was found to protect intestinal stem cells (ISCs) against inflammatory injury. Fut2ΔISC (ISC-specific Fut2 knockout) mouse models demonstrated that FUT2 depletion in ISCs aggravated epithelial damage and disrupted ISC growth and proliferation by escalating LPS-induced endoplasmic reticulum (ER) stress and initiating the IRE1/TRAF2/ASK1/JNK branch of the unfolded protein response (UPR) [1].

In conclusion, HYOU1 plays a crucial role in protecting cells from stress-related damage, as seen in the protection of ISCs against inflammatory injury. The use of gene-knockout mouse models, like the Fut2ΔISC mice, has been instrumental in uncovering these functions, providing insights into potential therapeutic strategies for intestinal injury disorders.