Itga2-flox Mouse
Common Name
Itga2-flox
제품 ID
S-CKO-03149
Backgroud
C57BL/6JCya
품종 계통계통 ID
CKOCMP-16398-Itga2-B6J-VA
상태
이 마우스 계통을 논문에서 사용할 경우, “Itga2-flox Mouse (카탈로그 번호 S-CKO-03149)은 Cyagen에서 구입하였습니다.”라고 명시해 주시기 바랍니다.
구매 가능한 제품 종류
연령
Genotype
성별
수량
표준 제공 조건은 최소 3마리의 이형접합(heterozygous) 보균자를 보장합니다. 동형접합(homozygous) 보균자 및/또는 특정 성별에 대한 브리딩 서비스도 제공됩니다.
기본 정보
품종 계통
Itga2-flox
품종 계통계통 ID
CKOCMP-16398-Itga2-B6J-VA
유전자명
제품 ID
S-CKO-03149
유전자 별칭
DX5, GPIa, CD49B
배경
C57BL/6JCya
NCBI ID
변형 내용
Conditional knockout
염색체
Chr 13
Phenotype
Datasheet
적용 분야
--
품종 계통 설명
Ensembl 전사체 ID
ENSMUST00000056117
NCBI 전사체 ID
NM_008396
타겟 영역
Exon 2
유효 영역 크기
~1.7 kb
유전자 연구 개요
Itga2, also known as CD49b or VLA-2, is the alpha subunit of the transmembrane collagen receptor integrin alpha-2/beta-1. It is involved in cell adhesion, and its associated pathways may include those related to DNA damage response, immune regulation, and extracellular matrix-mediated cell-cell interactions. It plays an important role in various biological processes such as cell growth, invasion, and DNA repair, and is thus of great biological significance. Genetic models, like KO/CKO mouse models, can be valuable for studying its functions [1,5].
In pancreatic cancer, overexpression of Itga2 inhibits DNA repair by restraining the recruitment of DNA-PKcs to Ku70/80 heterodimer in the non-homologous end joining (NHEJ) pathway, conferring sensitivity to radiotherapy. It also affects immune regulation by influencing the expression of MET, PD-L1, CD4, and CD8, and induces STING expression by degrading DNMT1 [1,2,5]. In glioblastoma, Itga2 is a potential nanotherapeutic target as its antibody blockade impedes cell migration. In colorectal cancer, it enhances 5-fluorouracil resistance, and this can be overcome by selumetinib [3,4]. In esophageal squamous cell carcinoma, overexpression of Itga2 promotes tumor cell proliferation, invasion, migration, and epithelial-mesenchymal transition (EMT) via the FAK/AKT signaling pathway [6]. In intrahepatic cholangiocarcinoma, elevated Itga2 expression promotes collagen type I-induced clonogenic growth. In thyroid carcinoma, hypomethylation-mediated overexpression of Itga2 stimulates cell invasion and migration. In glioma, Itga2 promotes glioma stem cell (GSC) invasion and EMT by activating STAT3 phosphorylation [7,8,9].
In conclusion, Itga2 has diverse functions in multiple biological processes and disease conditions. Through model-based research, especially KO/CKO mouse models, it has been revealed that Itga2 is involved in DNA repair, immune regulation, cell growth, invasion, and migration in various cancers, providing potential therapeutic targets for these diseases.
References：
1. Zhou, Chen, Li, Shoukang, Bin, Kaijian, Wu, Heshui, Zhou, Yingke. 2022. ITGA2 overexpression inhibits DNA repair and confers sensitivity to radiotherapies in pancreatic cancer. In Cancer letters, 547, 215855. doi:10.1016/j.canlet.2022.215855. https://pubmed.ncbi.nlm.nih.gov/35998796/
2. Jin, Liquan, Duan, Yaoqiang, Li, Xiaoxi, Chen, Yiming, Tan, Yunbo. 2023. High expression ITGA2 affects the expression of MET, PD-L1, CD4 and CD8 with the immune microenvironment in pancreatic cancer patients. In Frontiers in immunology, 14, 1209367. doi:10.3389/fimmu.2023.1209367. https://pubmed.ncbi.nlm.nih.gov/37881431/
3. Guo, Peng, Moses-Gardner, Alexander, Huang, Jing, Smith, Edward R, Moses, Marsha A. 2019. ITGA2 as a potential nanotherapeutic target for glioblastoma. In Scientific reports, 9, 6195. doi:10.1038/s41598-019-42643-7. https://pubmed.ncbi.nlm.nih.gov/30996239/
4. Qin, Jian, Hu, Shangshang, Lou, Jinwei, Pan, Yuqin, Wang, Shukui. 2024. Selumetinib overcomes ITGA2-induced 5-fluorouracil resistance in colorectal cancer. In International immunopharmacology, 137, 112487. doi:10.1016/j.intimp.2024.112487. https://pubmed.ncbi.nlm.nih.gov/38889513/
5. Meng, Junpeng, Cai, Hongkun, Sun, Yan, Wu, Heshui, Ren, Dianyun. 2022. ITGA2 induces STING expression in pancreatic cancer by inducing DNMT1 degradation. In Cellular oncology (Dordrecht, Netherlands), 45, 1421-1434. doi:10.1007/s13402-022-00731-3. https://pubmed.ncbi.nlm.nih.gov/36331797/
6. Huang, Wei, Zhu, Ju, Shi, Haoming, Wu, Qingchen, Zhang, Cheng. 2021. ITGA2 Overexpression Promotes Esophageal Squamous Cell Carcinoma Aggression via FAK/AKT Signaling Pathway. In OncoTargets and therapy, 14, 3583-3596. doi:10.2147/OTT.S302028. https://pubmed.ncbi.nlm.nih.gov/34113124/
7. Rattanasinchai, Chotirat, Navasumrit, Panida, Ruchirawat, Mathuros. 2022. Elevated ITGA2 expression promotes collagen type I-induced clonogenic growth of intrahepatic cholangiocarcinoma. In Scientific reports, 12, 22429. doi:10.1038/s41598-022-26747-1. https://pubmed.ncbi.nlm.nih.gov/36575207/
8. Chen, Hong, Zhang, Chunying, Li, Yanbing, Chen, Chunyou. 2022. Hypomethylation-mediated overexpression of ITGA2 stimulates cell invasion and migration of thyroid carcinoma. In Histology and histopathology, 38, 787-796. doi:10.14670/HH-18-552. https://pubmed.ncbi.nlm.nih.gov/36420922/
9. Zhang, Jin, Li, Ruinan, Zhang, Haibin, Wang, Shanshan, Zhao, Yuanli. . ITGA2 as a prognostic factor of glioma promotes GSCs invasion and EMT by activating STAT3 phosphorylation. In Carcinogenesis, 45, 235-246. doi:10.1093/carcin/bgad096. https://pubmed.ncbi.nlm.nih.gov/38142122/
품질 관리 기준
정자 검사
동결 보존 전: 정자 농도 측정 및 정자 생존율 평가.
동결 보존 후: 각 배치에서 동결 보존된 정자 바이알 1개를 선택하여 체외수정(in vitro fertilization)에 사용합니다.
Environmental Standards:
SPFAvailable Region:
GlobalSource:
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