Kcnmb1-flox Mouse
Common Name
Kcnmb1-flox
제품 ID
S-CKO-03240
Backgroud
C57BL/6JCya
품종 계통계통 ID
CKOCMP-16533-Kcnmb1-B6J-VA
상태
이 마우스 계통을 논문에서 사용할 경우, “Kcnmb1-flox Mouse (카탈로그 번호 S-CKO-03240)은 Cyagen에서 구입하였습니다.”라고 명시해 주시기 바랍니다.
구매 가능한 제품 종류
연령
Genotype
성별
수량
표준 제공 조건은 최소 3마리의 이형접합(heterozygous) 보균자를 보장합니다. 동형접합(homozygous) 보균자 및/또는 특정 성별에 대한 브리딩 서비스도 제공됩니다.
기본 정보
품종 계통
Kcnmb1-flox
품종 계통계통 ID
CKOCMP-16533-Kcnmb1-B6J-VA
유전자명
제품 ID
S-CKO-03240
유전자 별칭
BKbeta1
배경
C57BL/6JCya
NCBI ID
변형 내용
Conditional knockout
염색체
Chr 11
Phenotype
Datasheet
적용 분야
--
품종 계통 설명
Ensembl 전사체 ID
ENSMUST00000020362
NCBI 전사체 ID
NM_031169.4
타겟 영역
Exon 2~3
유효 영역 크기
~2.6 kb
유전자 연구 개요
KCNMB1, encoding the β1 subunit of the large-conductance calcium-activated potassium (BK) channel, is crucial for regulating BK channel activity. The BK channels are involved in various physiological processes such as smooth muscle contraction, neurotransmitter release, and regulation of vascular tone [2,3,4,5,6,7,8,9]. Genetic models can help understand its function and its role in related biological pathways and diseases.
In a meta-analysis, the dominant model of the KCNMB1 E65K polymorphism was significantly associated with essential hypertension risk in Asians, suggesting it might be a susceptibility factor [1]. In Kcnmb1-/-mice, they are hypertensive, volume expanded, and have reduced urinary K and Na clearances, with the majority of hypertension due to aldosteronism from renal potassium retention and hyperkalemia [7]. Fibroblasts from patients with idiopathic pulmonary fibrosis (IPF) show increased KCNMB1 expression, and knockdown of KCNMB1 attenuates their ability to contract collagen gels, indicating a role in myofibroblast differentiation [2].
In conclusion, KCNMB1 plays a vital role in regulating BK channel activity, which impacts various physiological functions. Studies using KO mouse models have revealed its significance in diseases like hypertension and pulmonary fibrosis. Understanding KCNMB1 through these models provides insights into disease mechanisms and potential therapeutic targets for these conditions.
References：
1. Zhancheng, Wang, Wenhui, Ji, Yun, Jiang, Yan, Shen, Jin, Li. . The dominant models of KCNJ11 E23K and KCNMB1 E65K are associated with essential hypertension (EH) in Asian: Evidence from a meta-analysis. In Medicine, 98, e15828. doi:10.1097/MD.0000000000015828. https://pubmed.ncbi.nlm.nih.gov/31169684/
2. Scruggs, Anne M, Grabauskas, Gintautas, Huang, Steven K. . The Role of KCNMB1 and BK Channels in Myofibroblast Differentiation and Pulmonary Fibrosis. In American journal of respiratory cell and molecular biology, 62, 191-203. doi:10.1165/rcmb.2019-0163OC. https://pubmed.ncbi.nlm.nih.gov/31486669/
3. Bukiya, Anna N, Leo, M Dennis, Jaggar, Jonathan H, Dopico, Alex M. 2021. Cholesterol activates BK channels by increasing KCNMB1 protein levels in the plasmalemma. In The Journal of biological chemistry, 296, 100381. doi:10.1016/j.jbc.2021.100381. https://pubmed.ncbi.nlm.nih.gov/33556372/
4. Son, Yonghae, Chun, Wonjoo, Ahn, Yong-Tae, Lee, Chu, An, Won G. 2015. 7-Ketocholesterol induces the reduction of KCNMB1 in atherosclerotic blood vessels. In Biochemical and biophysical research communications, 457, 324-7. doi:10.1016/j.bbrc.2014.12.109. https://pubmed.ncbi.nlm.nih.gov/25576871/
5. Mysiewicz, Steven, North, Kelsey C, Moreira, Luiz, Bukiya, Anna N, Dopico, Alex M. 2023. Interspecies and regional variability of alcohol action on large cerebral arteries: regulation by KCNMB1 proteins. In American journal of physiology. Regulatory, integrative and comparative physiology, 324, R480-R496. doi:10.1152/ajpregu.00103.2022. https://pubmed.ncbi.nlm.nih.gov/36717168/
6. Beitelshees, Amber L, Gong, Yan, Wang, Danxin, Pepine, Carl J, Johnson, Julie A. . KCNMB1 genotype influences response to verapamil SR and adverse outcomes in the INternational VErapamil SR/Trandolapril STudy (INVEST). In Pharmacogenetics and genomics, 17, 719-29. doi:. https://pubmed.ncbi.nlm.nih.gov/17700361/
7. Grimm, P Richard, Irsik, Debra L, Settles, Deann C, Holtzclaw, J David, Sansom, Steven C. 2009. Hypertension of Kcnmb1-/- is linked to deficient K secretion and aldosteronism. In Proceedings of the National Academy of Sciences of the United States of America, 106, 11800-5. doi:10.1073/pnas.0904635106. https://pubmed.ncbi.nlm.nih.gov/19556540/
8. Toro, B, Cox, N, Wilson, R J, Toro, L, Zarei, M M. 2006. KCNMB1 regulates surface expression of a voltage and Ca2+-activated K+ channel via endocytic trafficking signals. In Neuroscience, 142, 661-9. doi:. https://pubmed.ncbi.nlm.nih.gov/16908104/
9. Ahn, Yong-Tae, Kim, Yu-Mee, Adams, Eloa, Alvira, Cristina M, Cornfield, David N. 2011. Hypoxia-inducible factor-1α regulates KCNMB1 expression in human pulmonary artery smooth muscle cells. In American journal of physiology. Lung cellular and molecular physiology, 302, L352-9. doi:10.1152/ajplung.00302.2011. https://pubmed.ncbi.nlm.nih.gov/22114151/
품질 관리 기준
정자 검사
동결 보존 전: 정자 농도 측정 및 정자 생존율 평가.
동결 보존 후: 각 배치에서 동결 보존된 정자 바이알 1개를 선택하여 체외수정(in vitro fertilization)에 사용합니다.
Environmental Standards:
SPFAvailable Region:
GlobalSource:
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