Cmpk2-flox Mouse
Common Name
Cmpk2-flox
제품 ID
S-CKO-06500
Backgroud
C57BL/6JCya
품종 계통계통 ID
CKOCMP-22169-Cmpk2-B6J-VA
상태
이 마우스 계통을 논문에서 사용할 경우, “Cmpk2-flox Mouse (카탈로그 번호 S-CKO-06500)은 Cyagen에서 구입하였습니다.”라고 명시해 주시기 바랍니다.
구매 가능한 제품 종류
연령
Genotype
성별
수량
표준 제공 조건은 최소 3마리의 이형접합(heterozygous) 보균자를 보장합니다. 동형접합(homozygous) 보균자 및/또는 특정 성별에 대한 브리딩 서비스도 제공됩니다.
기본 정보
품종 계통
Cmpk2-flox
품종 계통계통 ID
CKOCMP-22169-Cmpk2-B6J-VA
유전자명
제품 ID
S-CKO-06500
유전자 별칭
TDKI, Tyki, 1200004E04Rik
배경
C57BL/6JCya
NCBI ID
변형 내용
Conditional knockout
염색체
Chr 12
Phenotype
Datasheet
적용 분야
--
품종 계통 설명
Ensembl 전사체 ID
ENSMUST00000020969
NCBI 전사체 ID
NM_020557
타겟 영역
Exon 2
유효 영역 크기
~1.4 kb
유전자 연구 개요
Cmpk2, short for cytidine/uridine monophosphate kinase 2, is an essential mitochondria-associated gene. It regulates mtDNA replication and is involved in multiple pathways, such as the NLRP3 inflammasome-related inflammatory pathway [1,2,3,6,7,8]. It also has antiviral functions against various viruses including Zika virus, flaviviruses, and coronaviruses [5,9]. Its expression is associated with many biological processes and disease conditions, making it an important target for research.
In ischemic stroke, microglia/macrophage Cmpk2 knockdown using Cre recombination-dependent adeno-associated virus suppresses brain inflammatory responses, reduces infarcts, and improves neurological outcomes. It limits newly synthesized mtDNA and Ox-mtDNA formation, blocking NLRP3 inflammasome activation [1].
In spinal cord injury rats, AAV-mediated Cmpk2 knockdown or electroacupuncture-mediated Cmpk2 down-regulation inhibits NLRP3 activation, improves motor function [2].
In sepsis, dracorhodin, a Cmpk2 inhibitor, exerts anti-inflammatory effects by regulating the NLRP3 inflammasome via the LPS-induced Cmpk2 pathway [3].
In mice, homozygous Cmpk2-KO mice develop progressive calcification in the thalamus region, mimicking the brain calcification pathology in patients with biallelic CMPK2 variants [4].
In atherosclerosis mouse models, EC-GATA6 deletion decreases monocyte recruitment and atherosclerotic lesion formation through regulation of the CMPK2-Nlrp3 pathway [6].
In glucocorticoid-induced osteoporosis study, inhibiting Cmpk2 expression in preosteoblasts alleviates glucocorticoid-induced cellular senescence and promotes osteogenic differentiation [7].
In conclusion, Cmpk2 plays a crucial role in regulating mitochondrial function, inflammation, and antiviral responses. Gene knockout and conditional knockout mouse models have revealed its significant contributions in diseases such as ischemic stroke, spinal cord injury, sepsis, brain calcification, atherosclerosis, and glucocorticoid-induced osteoporosis, providing potential therapeutic targets for these conditions.
References：
1. Guan, Xin, Zhu, Sitong, Song, Jinqian, Xu, Xiaojun, Pang, Tao. 2024. Microglial CMPK2 promotes neuroinflammation and brain injury after ischemic stroke. In Cell reports. Medicine, 5, 101522. doi:10.1016/j.xcrm.2024.101522. https://pubmed.ncbi.nlm.nih.gov/38701781/
2. Chen, Yi, Wu, Lei, Shi, Mengting, Shao, XiaoMei, Ma, Ruijie. 2022. Electroacupuncture Inhibits NLRP3 Activation by Regulating CMPK2 After Spinal Cord Injury. In Frontiers in immunology, 13, 788556. doi:10.3389/fimmu.2022.788556. https://pubmed.ncbi.nlm.nih.gov/35401582/
3. Zhang, Wendan, Jiang, Honghong, Huang, Pengli, Liu, Sanhong, Zhang, Weidong. . Dracorhodin targeting CMPK2 attenuates inflammation: A novel approach to sepsis therapy. In Clinical and translational medicine, 13, e1449. doi:10.1002/ctm2.1449. https://pubmed.ncbi.nlm.nih.gov/37859535/
4. Zhao, Miao, Su, Hui-Zhen, Zeng, Yi-Heng, Cheng, Xuewen, Chen, Wan-Jin. 2022. Loss of function of CMPK2 causes mitochondria deficiency and brain calcification. In Cell discovery, 8, 128. doi:10.1038/s41421-022-00475-2. https://pubmed.ncbi.nlm.nih.gov/36443312/
5. Pawlak, Joanna B, Hsu, Jack Chun-Chieh, Xia, Hongjie, Cresswell, Peter, Laurent-Rolle, Maudry. 2023. CMPK2 restricts Zika virus replication by inhibiting viral translation. In PLoS pathogens, 19, e1011286. doi:10.1371/journal.ppat.1011286. https://pubmed.ncbi.nlm.nih.gov/37075076/
6. Wu, Wenrun, Bao, Wenzhen, Chen, Xiaoli, Zhang, Yuzhen, Zhuang, Tao. 2023. Endothelial Gata6 deletion reduces monocyte recruitment and proinflammatory macrophage formation and attenuates atherosclerosis through Cmpk2-Nlrp3 pathways. In Redox biology, 64, 102775. doi:10.1016/j.redox.2023.102775. https://pubmed.ncbi.nlm.nih.gov/37339559/
7. Cao, Nianping, Wang, Zhihang, Huang, Chongjun, Xu, Ying, Tian, Ye. 2023. Cmpk2 regulates mitochondrial function in glucocorticoid-induced osteoblast senescence and affects glucocorticoid-inhibited osteoblast differentiation. In Archives of gerontology and geriatrics, 114, 105080. doi:10.1016/j.archger.2023.105080. https://pubmed.ncbi.nlm.nih.gov/37269696/
8. Jin, Li, Chen, Qiyue, Hu, Ke, Qi, Weizhong, Yu, Qinghong. 2024. The FTO-CMPK2 Pathway in Fibroblast-like Synoviocytes Modulates Rheumatoid Arthritis Synovial Inflammation and Cartilage Homeostasis via mtDNA Regulation. In International journal of biological sciences, 20, 1617-1633. doi:10.7150/ijbs.90677. https://pubmed.ncbi.nlm.nih.gov/38481810/
9. Zhu, Mingjun, Lv, Jiahuang, Wang, Wei, Ding, Siyuan, Li, Bin. 2023. CMPK2 is a host restriction factor that inhibits infection of multiple coronaviruses in a cell-intrinsic manner. In PLoS biology, 21, e3002039. doi:10.1371/journal.pbio.3002039. https://pubmed.ncbi.nlm.nih.gov/36930652/
품질 관리 기준
정자 검사
동결 보존 전: 정자 농도 측정 및 정자 생존율 평가.
동결 보존 후: 각 배치에서 동결 보존된 정자 바이알 1개를 선택하여 체외수정(in vitro fertilization)에 사용합니다.
Environmental Standards:
SPFAvailable Region:
GlobalSource:
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