Wasf2-flox Mouse
Common Name
Wasf2-flox
제품 ID
S-CKO-08467
Backgroud
C57BL/6NCya
품종 계통계통 ID
CKOCMP-242687-Wasf2-B6N-VA
상태
이 마우스 계통을 논문에서 사용할 경우, “Wasf2-flox Mouse (카탈로그 번호 S-CKO-08467)은 Cyagen에서 구입하였습니다.”라고 명시해 주시기 바랍니다.
구매 가능한 제품 종류
연령
Genotype
성별
수량
표준 제공 조건은 최소 3마리의 이형접합(heterozygous) 보균자를 보장합니다. 동형접합(homozygous) 보균자 및/또는 특정 성별에 대한 브리딩 서비스도 제공됩니다.
기본 정보
품종 계통
Wasf2-flox
품종 계통계통 ID
CKOCMP-242687-Wasf2-B6N-VA
유전자명
제품 ID
S-CKO-08467
유전자 별칭
WAVE2, D4Ertd13e
배경
C57BL/6NCya
NCBI ID
변형 내용
Conditional knockout
염색체
Chr 4
Phenotype
Datasheet
적용 분야
--
품종 계통 설명
Ensembl 전사체 ID
ENSMUST00000084241
NCBI 전사체 ID
NM_153423
타겟 영역
Exon 5
유효 영역 크기
~0.6 kb
유전자 연구 개요
WASF2, also known as Wiskott-Aldrich syndrome protein Verprolin-homologous protein 2 or WAVE2, is an integral member of the actin cytoskeleton pathway, playing a crucial role in cell motility, such as regulating actin reorganization during cell migration [1,3]. It is involved in multiple signaling pathways, including TGF-β signaling, Kras signaling, fatty acid metabolism, p53 pathways, and Wnt/β-catenin axis [1,4]. Genetic models can be valuable for studying its functions.
In various cancers, WASF2 shows different roles. In head and neck squamous cell carcinoma (HNSCC), hypermethylated WASF2 acts as a tumor suppressor. Its down-regulation in HNSCC tissues is related to elevated promoter methylation, and DNA methylation inhibitor can restore its mRNA expression. WASF2 expression is a favorable indicator for HPV-positive HNSCC, and its promoter methylation is detrimental to patient survival. Also, it is positively associated with tumor-killing immune cells [1]. In ovarian cancer, WASF2 is abnormally expressed, significantly correlated with overall survival and progression-free interval, and related to the tumor microenvironment, immune infiltration, TMB, MSI, and antitumor drugs sensitivity. Knockdown of WASF2 inhibits the proliferative, migratory and invasive capacity of ovarian cancer cells [2]. In hepatocellular carcinoma (HCC), WASF2 is overexpressed and hypomethylated, and its inactivation exerts anti-tumorigenic effects on HCC cells in vitro and in vivo [3]. In spinal cord glioma, EMX1 functions as a tumor inhibitor by suppressing WASF2-dependent activation of the Wnt/β-catenin axis [4].
In conclusion, WASF2 is essential in regulating cell motility through the actin cytoskeleton pathway. Its abnormal expression and epigenetic changes are closely associated with the development and prognosis of multiple cancers, such as HNSCC, ovarian cancer, HCC, and spinal cord glioma. Studies using various models help to understand its functions in these disease conditions, providing potential targets for cancer treatment.
References：
1. Zhang, Jianyun, Ding, Zhuang, Chen, Long, Qin, Haiyan. 2022. Hypermethylated WASF2: tumor suppressive role in head and neck squamous cell carcinoma. In Translational cancer research, 12, 78-92. doi:10.21037/tcr-22-1133. https://pubmed.ncbi.nlm.nih.gov/36760387/
2. Yang, Xiaofeng, Ding, Yuzhen, Sun, Lu, Huang, Zhengrui, Li, Ruiman. 2022. WASF2 Serves as a Potential Biomarker and Therapeutic Target in Ovarian Cancer: A Pan-Cancer Analysis. In Frontiers in oncology, 12, 840038. doi:10.3389/fonc.2022.840038. https://pubmed.ncbi.nlm.nih.gov/35359421/
3. Ahn, Hye Ri, Baek, Geum Ok, Yoon, Moon Gyeong, Eun, Jung Woo, Kim, Soon Sun. 2022. Hypomethylation-mediated upregulation of the WASF2 promoter region correlates with poor clinical outcomes in hepatocellular carcinoma. In Journal of experimental & clinical cancer research : CR, 41, 158. doi:10.1186/s13046-022-02365-7. https://pubmed.ncbi.nlm.nih.gov/35477411/
4. Han, Ziyin, Mou, Zufang, Jing, Yulong, Jiang, Rong, Sun, Tao. 2022. EMX1 functions as a tumor inhibitor in spinal cord glioma through transcriptional suppression of WASF2 and inactivation of the Wnt/β-catenin axis. In Brain and behavior, 12, e2684. doi:10.1002/brb3.2684. https://pubmed.ncbi.nlm.nih.gov/35849030/
품질 관리 기준
정자 검사
동결 보존 전: 정자 농도 측정 및 정자 생존율 평가.
동결 보존 후: 각 배치에서 동결 보존된 정자 바이알 1개를 선택하여 체외수정(in vitro fertilization)에 사용합니다.
Environmental Standards:
SPFAvailable Region:
GlobalSource:
Cyagen문의하기
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