Cpsf6-flox Mouse
Common Name
Cpsf6-flox
제품 ID
S-CKO-11219
Backgroud
C57BL/6NCya
품종 계통계통 ID
CKOCMP-432508-Cpsf6-B6N-VA
상태
이 마우스 계통을 논문에서 사용할 경우, “Cpsf6-flox Mouse (카탈로그 번호 S-CKO-11219)은 Cyagen에서 구입하였습니다.”라고 명시해 주시기 바랍니다.
구매 가능한 제품 종류
연령
Genotype
성별
수량
표준 제공 조건은 최소 3마리의 이형접합(heterozygous) 보균자를 보장합니다. 동형접합(homozygous) 보균자 및/또는 특정 성별에 대한 브리딩 서비스도 제공됩니다.
기본 정보
품종 계통
Cpsf6-flox
품종 계통계통 ID
CKOCMP-432508-Cpsf6-B6N-VA
유전자명
제품 ID
S-CKO-11219
유전자 별칭
CFIM, CFIM68, HPBRII-4, HPBRII-7, 4733401N12Rik
배경
C57BL/6NCya
NCBI ID
변형 내용
Conditional knockout
염색체
Chr 10
Phenotype
Datasheet
적용 분야
--
품종 계통 설명
Ensembl 전사체 ID
ENSMUST00000069168
NCBI 전사체 ID
NM_001013391
타겟 영역
Exon 4
유효 영역 크기
~0.6 kb
유전자 연구 개요
Cpsf6, also known as Cleavage and polyadenylation specificity factor subunit 6 or CFIm68, is a 68 kDa component of the mammalian cleavage factor I (CFIm) complex. It modulates mRNA alternative polyadenylation (APA), determining 3' untranslated region (UTR) length, an important gene expression control mechanism. Cpsf6 is also involved in the HIV-1 replication cycle, as it directly interacts with the HIV-1 core during infection [4].
In cancer cells, elevated Cpsf6 can lead to a systematic prolongation of 3' UTRs, yet its expression is typically higher in tumors than in healthy tissues. Cpsf6 can undergo liquid-liquid phase separation (LLPS), and elevated LLPS is associated with the preferential usage of the distal poly(A) sites. In lung adenocarcinoma, Cpsf6 elevation is attributed to BRCA1-disrupted R-loop accumulation in its 5' end, which leads to XBP1 3'UTR shortening, attenuating cisplatin-induced ER stress and elevating chemo-resistance. Inhibition of Cpsf6 expression in lung adenocarcinoma cell lines caused a significant reduction in cell proliferation, colony formation, and induced apoptosis. In hepatocellular carcinoma, Cpsf6 is upregulated and promotes tumorigenic activities. Depletion of Cpsf6 suppresses cell viability, colony formation, and the Warburg effect, and inhibits angiogenesis [1,2,3,6,7].
In the context of HIV-1 infection, preventing the formation of Cpsf6 condensates inhibits the infection of wild-type HIV-1. Also, cytoplasmic Cpsf6 regulates HIV-1 capsid trafficking and infection in a cyclophilin A-dependent manner [5,8].
In conclusion, Cpsf6 plays crucial roles in both cancer-related processes such as cell proliferation, apoptosis, chemo-resistance, and metabolism adaption, and in the HIV-1 replication cycle, including viral capsid trafficking and the formation of biomolecular condensates important for infection. Research on Cpsf6, especially through loss-of-function experiments, provides valuable insights into these disease areas, potentially guiding the development of new therapeutic strategies.
References：
1. Liu, Susu, Wu, Runze, Chen, Liutao, Fu, Yonggui, Xu, Anlong. 2023. CPSF6 regulates alternative polyadenylation and proliferation of cancer cells through phase separation. In Cell reports, 42, 113197. doi:10.1016/j.celrep.2023.113197. https://pubmed.ncbi.nlm.nih.gov/37777964/
2. Zhu, Chuandong, Xie, Yufeng, Li, Qiang, Yu, Zhengyuan, Chen, Jing. 2023. CPSF6-mediated XBP1 3'UTR shortening attenuates cisplatin-induced ER stress and elevates chemo-resistance in lung adenocarcinoma. In Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy, 68, 100933. doi:10.1016/j.drup.2023.100933. https://pubmed.ncbi.nlm.nih.gov/36821972/
3. Zu, Yukun, Wang, Dao, Ping, Wei, Sun, Wei. 2022. The roles of CPSF6 in proliferation, apoptosis and tumorigenicity of lung adenocarcinoma. In Aging, 14, 9300-9316. doi:10.18632/aging.204407. https://pubmed.ncbi.nlm.nih.gov/36446361/
4. Bialas, Katarzyna, Diaz-Griffero, Felipe. 2024. HIV-1-induced translocation of CPSF6 to biomolecular condensates. In Trends in microbiology, 32, 781-790. doi:10.1016/j.tim.2024.01.001. https://pubmed.ncbi.nlm.nih.gov/38267295/
5. Luchsinger, Charlotte, Lee, KyeongEun, Mardones, Gonzalo A, KewalRamani, Vineet N, Diaz-Griffero, Felipe. 2023. Formation of nuclear CPSF6/CPSF5 biomolecular condensates upon HIV-1 entry into the nucleus is important for productive infection. In Scientific reports, 13, 10974. doi:10.1038/s41598-023-37364-x. https://pubmed.ncbi.nlm.nih.gov/37414787/
6. Tan, Sheng, Zhang, Ming, Shi, Xinglong, Sun, Jielin, Zhao, Xiaodong. 2021. CPSF6 links alternative polyadenylation to metabolism adaption in hepatocellular carcinoma progression. In Journal of experimental & clinical cancer research : CR, 40, 85. doi:10.1186/s13046-021-01884-z. https://pubmed.ncbi.nlm.nih.gov/33648552/
7. Sim, Deok Yong, Lee, Hyo-Jung, Ahn, Chi-Hoon, Kim, Bonglee, Kim, Sung-Hoon. 2024. Negative Regulation of CPSF6 Suppresses the Warburg Effect and Angiogenesis Leading to Tumor Progression Via c-Myc Signaling Network: Potential Therapeutic Target for Liver Cancer Therapy. In International journal of biological sciences, 20, 3442-3460. doi:10.7150/ijbs.93462. https://pubmed.ncbi.nlm.nih.gov/38993554/
8. Zhong, Zhou, Ning, Jiying, Boggs, Emerson A, Watkins, Simon C, Ambrose, Zandrea. 2021. Cytoplasmic CPSF6 Regulates HIV-1 Capsid Trafficking and Infection in a Cyclophilin A-Dependent Manner. In mBio, 12, . doi:10.1128/mBio.03142-20. https://pubmed.ncbi.nlm.nih.gov/33758083/
품질 관리 기준
정자 검사
동결 보존 전: 정자 농도 측정 및 정자 생존율 평가.
동결 보존 후: 각 배치에서 동결 보존된 정자 바이알 1개를 선택하여 체외수정(in vitro fertilization)에 사용합니다.
Environmental Standards:
SPFAvailable Region:
GlobalSource:
Cyagen문의하기
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