Atf7ip-flox Mouse
Common Name
Atf7ip-flox
제품 ID
S-CKO-11722
Backgroud
C57BL/6JCya
품종 계통계통 ID
CKOCMP-54343-Atf7ip-B6J-VA
상태
이 마우스 계통을 논문에서 사용할 경우, “Atf7ip-flox Mouse (카탈로그 번호 S-CKO-11722)은 Cyagen에서 구입하였습니다.”라고 명시해 주시기 바랍니다.
구매 가능한 제품 종류
연령
Genotype
성별
수량
표준 제공 조건은 최소 3마리의 이형접합(heterozygous) 보균자를 보장합니다. 동형접합(homozygous) 보균자 및/또는 특정 성별에 대한 브리딩 서비스도 제공됩니다.
기본 정보
품종 계통
Atf7ip-flox
품종 계통계통 ID
CKOCMP-54343-Atf7ip-B6J-VA
유전자명
제품 ID
S-CKO-11722
유전자 별칭
AM, Mcaf1, 2610204M12Rik, 5830415B17Rik
배경
C57BL/6JCya
NCBI ID
변형 내용
Conditional knockout
염색체
Chr 6
Phenotype
Datasheet
적용 분야
--
품종 계통 설명
Ensembl 전사체 ID
ENSMUST00000032335
NCBI 전사체 ID
NM_019426
타겟 영역
Exon 2
유효 영역 크기
~2.2 kb
유전자 연구 개요
Atf7ip, the activating transcription factor 7 interacting protein, is an epigenetic regulator. It interacts with Setdb1, a lysine methyltransferase responsible for histone H3 lysine 9 (H3K9) methylation. Together, they play a crucial role in various biological processes through H3K9me3 modification and chromatin remodeling, influencing gene expression and cell differentiation [1,2,3,4,5,6,7,8].
In zebrafish, Atf7ip-deficient mutants show excessive myeloid differentiation with impaired hematopoietic stem and progenitor cell (HSPC) expansion, leading to a decline in T cells and erythroid lineage. Atf7ip regulates hematopoiesis via Setdb1-mediated H3K9me3 modification in hematopoietic regulatory genes, preventing premature myeloid differentiation. Also, loss of Atf7ip derepresses retrotransposons, triggering viral sensor signaling and stress-driven myelopoiesis and inflammation. In human leukemic cells, ATF7IP depletion represses cell growth and induces myeloid differentiation with retrotransposon-triggered inflammation [1].
In mice, T cell-specific deletion of ATF7ip enhances Il7r and Il2 expression in CD8+ T cells, leading to enhanced effector and memory responses, as ATF7ip represses these genes through H3K9me3 deposition at relevant loci [3]. Mice with T cell-specific deletion of Atf7ip have impaired Th17 differentiation due to aberrant IL-2 overproduction, as Atf7ip inhibits Il2 gene expression through H3K9me3 deposition in the Il2-Il21 intergenic region [5]. Disrupting Atf7ip in tumor cells restores tumor antigen expression, augments tumor immunogenicity, and leads to increased T-cell infiltration and tumor rejection [6].
In osteoblasts, Atf7ip inhibits differentiation. Atf7ip-deficient mice (Oc-Cre;Atf7ipf/f) show more bone formation. Atf7ip contributes to Setdb1's nucleus localization in osteoblasts and negatively regulates Sp7 expression [2].
In summary, Atf7ip, mainly through its interaction with Setdb1 and H3K9me3-related epigenetic regulation, is essential in hematopoiesis, immune cell function, and osteoblast differentiation. Gene-knockout mouse models have revealed its role in hematological malignancies, autoimmune diseases, cancer immunology, and bone-related conditions, providing potential targets for intervention in these disease areas.
References：
1. Wu, Jiaxin, Li, Juan, Chen, Kang, Xie, Peng, Zhong, Tao P. 2022. Atf7ip and Setdb1 interaction orchestrates the hematopoietic stem and progenitor cell state with diverse lineage differentiation. In Proceedings of the National Academy of Sciences of the United States of America, 120, e2209062120. doi:10.1073/pnas.2209062120. https://pubmed.ncbi.nlm.nih.gov/36577070/
2. Hu, Guoqin, Shi, Xian, Qu, Xiuxia, Liu, Huanliang, Wu, Yu. 2023. Atf7ip Inhibits Osteoblast Differentiation via Negative Regulation of the Sp7 Transcription Factor. In International journal of molecular sciences, 24, . doi:10.3390/ijms24054305. https://pubmed.ncbi.nlm.nih.gov/36901736/
3. Sin, Jun Hyung, Kashyap, Sujit, Acenas, Dante, Matloubian, Mehrdad, Waterfield, Michael R. 2022. ATF7ip Targets Transposable Elements for H3K9me3 Deposition to Modify CD8+ T Cell Effector and Memory Responses. In Journal of immunology (Baltimore, Md. : 1950), 208, 1155-1169. doi:10.4049/jimmunol.2100996. https://pubmed.ncbi.nlm.nih.gov/35110421/
4. Tsusaka, Takeshi, Shimura, Chikako, Shinkai, Yoichi. 2019. ATF7IP regulates SETDB1 nuclear localization and increases its ubiquitination. In EMBO reports, 20, e48297. doi:10.15252/embr.201948297. https://pubmed.ncbi.nlm.nih.gov/31576654/
5. Sin, Jun Hyung, Zuckerman, Cassandra, Cortez, Jessica T, Anderson, Mark S, Waterfield, Michael R. 2019. The epigenetic regulator ATF7ip inhibits Il2 expression, regulating Th17 responses. In The Journal of experimental medicine, 216, 2024-2037. doi:10.1084/jem.20182316. https://pubmed.ncbi.nlm.nih.gov/31217192/
6. Hu, Hai, Khodadadi-Jamayran, Alireza, Dolgalev, Igor, Li, Fei, Wong, Kwok-Kin. 2021. Targeting the Atf7ip-Setdb1 Complex Augments Antitumor Immunity by Boosting Tumor Immunogenicity. In Cancer immunology research, 9, 1298-1315. doi:10.1158/2326-6066.CIR-21-0543. https://pubmed.ncbi.nlm.nih.gov/34462284/
7. Kariapper, Leena, Marathe, Ila A, Niesman, Ashley B, Wysocki, Vicki H, Worden, Evan J. 2024. Setdb1 and Atf7IP form a hetero-trimeric complex that blocks Setdb1 nuclear export. In bioRxiv : the preprint server for biology, , . doi:10.1101/2024.12.23.630145. https://pubmed.ncbi.nlm.nih.gov/39764026/
8. Timms, Richard T, Tchasovnikarova, Iva A, Antrobus, Robin, Dougan, Gordon, Lehner, Paul J. . ATF7IP-Mediated Stabilization of the Histone Methyltransferase SETDB1 Is Essential for Heterochromatin Formation by the HUSH Complex. In Cell reports, 17, 653-659. doi:10.1016/j.celrep.2016.09.050. https://pubmed.ncbi.nlm.nih.gov/27732843/
품질 관리 기준
정자 검사
동결 보존 전: 정자 농도 측정 및 정자 생존율 평가.
동결 보존 후: 각 배치에서 동결 보존된 정자 바이알 1개를 선택하여 체외수정(in vitro fertilization)에 사용합니다.
Environmental Standards:
SPFAvailable Region:
GlobalSource:
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