Calcrl-flox Mouse
Common Name
Calcrl-flox
제품 ID
S-CKO-11816
Backgroud
C57BL/6JCya
품종 계통계통 ID
CKOCMP-54598-Calcrl-B6J-VA
상태
이 마우스 계통을 논문에서 사용할 경우, “Calcrl-flox Mouse (카탈로그 번호 S-CKO-11816)은 Cyagen에서 구입하였습니다.”라고 명시해 주시기 바랍니다.
구매 가능한 제품 종류
연령
Genotype
성별
수량
표준 제공 조건은 최소 3마리의 이형접합(heterozygous) 보균자를 보장합니다. 동형접합(homozygous) 보균자 및/또는 특정 성별에 대한 브리딩 서비스도 제공됩니다.
기본 정보
품종 계통
Calcrl-flox
품종 계통계통 ID
CKOCMP-54598-Calcrl-B6J-VA
유전자명
제품 ID
S-CKO-11816
유전자 별칭
CRLR
배경
C57BL/6JCya
유전자 공식 전체 명칭
calcitonin receptor-like
NCBI ID
변형 내용
Conditional knockout
염색체
Chr 2
Phenotype
Datasheet
적용 분야
--
품종 계통 설명
Ensembl 전사체 ID
ENSMUST00000074262
NCBI 전사체 ID
NM_018782
타겟 영역
Exon 6
유효 영역 크기
~1.9 kb
유전자 연구 개요
CALCRL, short for calcitonin receptor-like receptor, is a G-protein-coupled neuropeptide receptor. It is involved in various biological processes, and its associated pathways include cyclic AMP (cAMP) signaling pathway, which is activated when it forms a receptor complex with RAMP1 in response to ligands like calcitonin gene-related peptide (CGRP) [1,3,5,6]. It is of great biological importance in processes such as immune response, hematopoiesis, and drug-tolerance in cancer cells. Genetic models, like KO or CKO mouse models, are valuable for studying its functions.
In the spleen, nociceptors release CGRP which acts on B cells through the CALCRL-RAMP1 complex via the cAMP pathway, promoting germinal center response and humoral immunity [1]. In acute myeloid leukemia (AML), CALCRL is expressed in relapse-initiating drug-tolerant cells. Knockdown of CALCRL impairs leukemic growth, decreases the frequency of leukemia-initiating cells, and sensitizes cells to cytarabine [2]. In tendon-bone healing, overexpression of Calcrl in mice improves osteogenic differentiation, type H vessel and nerve fiber intensity at the tendon-bone interface, as well as gait and biomechanical performance [3]. In AML/ETO + AML patients, lower CALCRL expression is associated with longer survival, and its expression decreases after chemotherapy-induced remission [4]. In AML cells, overexpression of CALCRL confers resistance to daunorubicin through up-regulation of the XRCC5/TYK2/JAK1 pathway [7]. In glioma, interfering with CALCRL expression in U87 cells (a glioma cell line) inhibits proliferation, promotes apoptosis, and is related to prognosis, especially in low-grade gliomas [8].
In conclusion, CALCRL plays essential roles in multiple biological processes. Through model-based research, its significance in immune regulation, cancer drug-resistance, and tissue repair has been revealed. In particular, in diseases like AML and glioma, studies using KO/CKO mouse models could potentially provide insights into novel therapeutic strategies targeting CALCRL.
References：
1. Wu, Min, Song, Guangping, Li, Jianing, Zhang, Yucheng, Li, Huiyan. 2024. Innervation of nociceptor neurons in the spleen promotes germinal center responses and humoral immunity. In Cell, 187, 2935-2951.e19. doi:10.1016/j.cell.2024.04.027. https://pubmed.ncbi.nlm.nih.gov/38772371/
2. Larrue, Clément, Guiraud, Nathan, Mouchel, Pierre-Luc, Récher, Christian, Sarry, Jean-Emmanuel. 2021. Adrenomedullin-CALCRL axis controls relapse-initiating drug tolerant acute myeloid leukemia cells. In Nature communications, 12, 422. doi:10.1038/s41467-020-20717-9. https://pubmed.ncbi.nlm.nih.gov/33462236/
3. Zhao, Xibang, Wu, Guanfu, Zhang, Jing, Yu, Ziyi, Wang, Jiali. 2024. Activation of CGRP receptor-mediated signaling promotes tendon-bone healing. In Science advances, 10, eadg7380. doi:10.1126/sciadv.adg7380. https://pubmed.ncbi.nlm.nih.gov/38457499/
4. Wang, Rongrong, Li, Miao, Bai, Yujia, Jiao, Yang, Qi, Xiaofei. 2022. CALCRL Gene is a Suitable Prognostic Factor in AML/ETO+ AML Patients. In Journal of oncology, 2022, 3024360. doi:10.1155/2022/3024360. https://pubmed.ncbi.nlm.nih.gov/35342399/
5. Hou, Yu, Sun, Linyu, LaFleur, Martin W, Sharpe, Arlene H, Kuchroo, Vijay K. 2024. Neuropeptide signalling orchestrates T cell differentiation. In Nature, 635, 444-452. doi:10.1038/s41586-024-08049-w. https://pubmed.ncbi.nlm.nih.gov/39415015/
6. Gao, Xin, Zhang, Dachuan, Xu, Chunliang, Caron, Kathleen M, Frenette, Paul S. 2020. Nociceptive nerves regulate haematopoietic stem cell mobilization. In Nature, 589, 591-596. doi:10.1038/s41586-020-03057-y. https://pubmed.ncbi.nlm.nih.gov/33361809/
7. Tang, Shanhao, Li, Shuangyue, Shi, Xiaowei, Wu, An, Ouyang, Guifang. 2023. CALCRL induces resistance to daunorubicin in acute myeloid leukemia cells through upregulation of XRCC5/TYK2/JAK1 pathway. In Anti-cancer drugs, 35, 163-176. doi:10.1097/CAD.0000000000001547. https://pubmed.ncbi.nlm.nih.gov/37948318/
8. Gu, Shengcai, Shu, Lei, Zhou, Lv, Gao, Peng, Cheng, Hongwei. . Interfering with CALCRL expression inhibits glioma proliferation, promotes apoptosis, and predicts prognosis in low-grade gliomas. In Annals of translational medicine, 10, 1277. doi:10.21037/atm-22-5154. https://pubmed.ncbi.nlm.nih.gov/36618798/
품질 관리 기준
정자 검사
동결 보존 전: 정자 농도 측정 및 정자 생존율 평가.
동결 보존 후: 각 배치에서 동결 보존된 정자 바이알 1개를 선택하여 체외수정(in vitro fertilization)에 사용합니다.
Environmental Standards:
SPFAvailable Region:
GlobalSource:
Cyagen출판물
Immunity
2025-06-16
Nociceptor-derived CGRP enhances dermal type I conventional dendritic cell function to drive autoreactive CD8+ T cell responses in vitiligo
자세히 보기
Cell
2024-05-19
Innervation of nociceptor neurons in the spleen promotes germinal center responses and humoral immunity
자세히 보기
Cellular & Molecular Immunology
2023-01
The neuropeptide CGRP enters the macrophage cytosol to suppress the NLRP3 inflammasome during pulmonary infection
자세히 보기
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