Trmt6-flox Mouse
Common Name
Trmt6-flox
제품 ID
S-CKO-13391
Backgroud
C57BL/6NCya
품종 계통계통 ID
CKOCMP-66926-Trmt6-B6N-VA
상태
이 마우스 계통을 논문에서 사용할 경우, “Trmt6-flox Mouse (카탈로그 번호 S-CKO-13391)은 Cyagen에서 구입하였습니다.”라고 명시해 주시기 바랍니다.
구매 가능한 제품 종류
연령
Genotype
성별
수량
표준 제공 조건은 최소 3마리의 이형접합(heterozygous) 보균자를 보장합니다. 동형접합(homozygous) 보균자 및/또는 특정 성별에 대한 브리딩 서비스도 제공됩니다.
기본 정보
품종 계통
Trmt6-flox
품종 계통계통 ID
CKOCMP-66926-Trmt6-B6N-VA
유전자명
제품 ID
S-CKO-13391
유전자 별칭
CGI-09, mKIAA1153, 3300001M20Rik
배경
C57BL/6NCya
NCBI ID
변형 내용
Conditional knockout
염색체
Chr 2
Phenotype
Datasheet
적용 분야
--
품종 계통 설명
Ensembl 전사체 ID
ENSMUST00000039554
NCBI 전사체 ID
NM_175113
타겟 영역
Exon 2
유효 영역 크기
~0.6 kb
유전자 연구 개요
TRMT6, short for tRNA methyltransferase 6, is an enzyme that catalyzes N1-methyladenosine (m1A), a reversible modification in various RNAs such as tRNA, mRNA, rRNA, and lncRNA. This modification is crucial as it participates in regulating RNA processing, structure, and functions. The TRMT6-TRMT61A complex, in particular, is involved in the m1A methylation of tRNA, which impacts diverse biological processes and is associated with multiple disease-related pathways [1,2,4,5,6,7,9].
In aged murine hematopoietic stem cells (HSCs), an increase in the TRMT6-TRMT61A complex due to declined CRL4DCAF1-mediated ubiquitination degradation signaling impairs HSCs through 3'-tiRNA-Leu-CAG and the RIPK1-RIPK3-MLKL-mediated necroptosis cascade [2]. In hepatocellular carcinoma (HCC), TRMT6 is overexpressed, promoting cell proliferation both in vivo and in vitro through the PI3K/AKT/mTOR axis [3]. Also, in HCC, TRMT6/TRMT61A-mediated m1A methylation in tRNA drives tumourigenesis by regulating cholesterol metabolism [4]. In bladder cancer, depletion of TRMT6/TRMT61A reduces proliferation and resistance against cellular stress [7]. In addition, the rs236110 C > A polymorphism in the TRMT6 gene increases the risk of Wilms tumor [8].
In conclusion, TRMT6, mainly as part of the TRMT6-TRMT61A complex, plays a significant role in m1A methylation of tRNA, influencing diverse biological processes. Model-based research, especially studies using KO/CKO mouse models, has revealed its importance in diseases like HSC aging-related impairments, hepatocellular carcinoma, bladder cancer, and Wilms tumor, providing insights into disease mechanisms and potential therapeutic targets.
References：
1. Su, Zhangli, Monshaugen, Ida, Wilson, Briana, Ougland, Rune, Dutta, Anindya. 2022. TRMT6/61A-dependent base methylation of tRNA-derived fragments regulates gene-silencing activity and the unfolded protein response in bladder cancer. In Nature communications, 13, 2165. doi:10.1038/s41467-022-29790-8. https://pubmed.ncbi.nlm.nih.gov/35444240/
2. He, Hanqing, Wang, Yuqian, Zhang, Xiaoting, Yi, Chengqi, Wang, Jianwei. 2024. Age-related noncanonical TRMT6-TRMT61A signaling impairs hematopoietic stem cells. In Nature aging, 4, 213-230. doi:10.1038/s43587-023-00556-1. https://pubmed.ncbi.nlm.nih.gov/38233630/
3. Ye, Yanqing, Liu, Maosheng, Wu, Fengfei, Xie, Fang, Bai, Lan. 2023. TRMT6 promotes hepatocellular carcinoma progression through the PI3K/AKT signaling pathway. In European journal of medical research, 28, 48. doi:10.1186/s40001-022-00951-1. https://pubmed.ncbi.nlm.nih.gov/36707905/
4. Wang, Yanying, Wang, Jing, Li, Xiaoyu, Yi, Chengqi, Fan, Zusen. 2021. N1-methyladenosine methylation in tRNA drives liver tumourigenesis by regulating cholesterol metabolism. In Nature communications, 12, 6314. doi:10.1038/s41467-021-26718-6. https://pubmed.ncbi.nlm.nih.gov/34728628/
5. Zuo, Hongna, Wu, Aiwei, Wang, Mingwei, Hong, Liquan, Wang, Hu. 2024. tRNA m1A modification regulate HSC maintenance and self-renewal via mTORC1 signaling. In Nature communications, 15, 5706. doi:10.1038/s41467-024-50110-9. https://pubmed.ncbi.nlm.nih.gov/38977676/
6. Li, Jiexin, Zhang, Haisheng, Wang, Hongsheng. 2022. N1-methyladenosine modification in cancer biology: Current status and future perspectives. In Computational and structural biotechnology journal, 20, 6578-6585. doi:10.1016/j.csbj.2022.11.045. https://pubmed.ncbi.nlm.nih.gov/36467585/
7. Monshaugen, Ida, Luna, Luisa, Rhodes, Jayden, Klungland, Arne, Ougland, Rune. 2024. Depletion of the m1A writer TRMT6/TRMT61A reduces proliferation and resistance against cellular stress in bladder cancer. In Frontiers in oncology, 13, 1334112. doi:10.3389/fonc.2023.1334112. https://pubmed.ncbi.nlm.nih.gov/38304034/
8. Chang, Xiaofeng, Zhu, Jinhong, Hua, Rui-Xi, He, Jing, Wang, Huanmin. 2023. TRMT6 gene rs236110 C > A polymorphism increases the risk of Wilms tumor. In Gene, 882, 147646. doi:10.1016/j.gene.2023.147646. https://pubmed.ncbi.nlm.nih.gov/37473973/
9. Liu, Yongbo, Zhou, Jing, Li, Xiaoyu, Wu, Yuzhang, Li, Hua-Bing. 2022. tRNA-m1A modification promotes T cell expansion via efficient MYC protein synthesis. In Nature immunology, 23, 1433-1444. doi:10.1038/s41590-022-01301-3. https://pubmed.ncbi.nlm.nih.gov/36138184/
품질 관리 기준
정자 검사
동결 보존 전: 정자 농도 측정 및 정자 생존율 평가.
동결 보존 후: 각 배치에서 동결 보존된 정자 바이알 1개를 선택하여 체외수정(in vitro fertilization)에 사용합니다.
Environmental Standards:
SPFAvailable Region:
GlobalSource:
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