Myct1-flox Mouse

MYCT1, also known as MTLC, is a crucial gene with diverse functions. As a novel c-Myc target gene, it is regulated by multiple transcription factors. It plays essential roles in various biological processes such as cell proliferation, apoptosis, migration, and differentiation, and is involved in tumor angiogenesis and immune response modulation within the tumor microenvironment [2].

In human haematopoietic stem cells (HSCs), lentivirus-mediated knockdown of MYCT1 prevented HSPC expansion and engraftment, while restoring its expression improved these processes. Single-cell RNA sequencing showed that MYCT1 governs regulatory programmes and cellular properties for HSC stemness, and its loss led to excessive endocytosis and hyperactive signalling [1].

In mice, global inactivation of Myct1 led to progressive glycogen accumulation in the liver, as MYCT1 promoted the translation efficiency of enzymes related to the glycogen shunt in a RACK1-dependent manner [3]. In diffuse large B-cell lymphoma, MYCT1 inhibited hematopoiesis by suppressing RUNX1 transcription [4].

In conclusion, MYCT1 is a multi-functional gene. Its study through gene-knockout models has revealed its significance in areas such as haematopoiesis, metabolism, and cancer development. These findings provide valuable insights into understanding biological processes and developing potential therapeutic strategies for related diseases.