Vsig1-flox Mouse
Common Name
Vsig1-flox
제품 ID
S-CKO-16865
Backgroud
C57BL/6JCya
품종 계통계통 ID
CKOCMP-78789-Vsig1-B6J-VA
상태
이 마우스 계통을 논문에서 사용할 경우, “Vsig1-flox Mouse (카탈로그 번호 S-CKO-16865)은 Cyagen에서 구입하였습니다.”라고 명시해 주시기 바랍니다.
구매 가능한 제품 종류
연령
Genotype
성별
수량
표준 제공 조건은 최소 3마리의 이형접합(heterozygous) 보균자를 보장합니다. 동형접합(homozygous) 보균자 및/또는 특정 성별에 대한 브리딩 서비스도 제공됩니다.
기본 정보
품종 계통
Vsig1-flox
품종 계통계통 ID
CKOCMP-78789-Vsig1-B6J-VA
유전자명
제품 ID
S-CKO-16865
유전자 별칭
1700062D20Rik, 4930405J24Rik
배경
C57BL/6JCya
NCBI ID
변형 내용
Conditional knockout
염색체
Chr X
Phenotype
Datasheet
적용 분야
--
품종 계통 설명
Ensembl 전사체 ID
ENSMUST00000033806
NCBI 전사체 ID
NM_030181
타겟 영역
Exon 2
유효 영역 크기
~1.0 kb
유전자 연구 개요
Vsig1, a cell-cell adhesion molecule, is a member of the immunoglobulin superfamily. It is preferentially expressed in the stomach, testis, and certain cancers such as gastric, esophageal, and ovarian cancers [4]. In the stomach, it is involved in the proper differentiation of glandular gastric epithelia, being restricted to the adherens junction of the glandular epithelium [4]. In testis, it is located on the testicular germ cell surface and its extracellular domain interacts with Sertoli cell membrane protein while its PDZ-binding motif forms a complex with ZO-1 [7].
In gastric cancer, several studies have explored its role. Loss of VSIG1 expression is significantly correlated with poor overall and disease-free survival, and negative VSIG1 expression is related to tumor size, T, and M stage, suggesting it may be a prognostic marker and potential therapeutic target [5]. Also, its membrane-cytoplasmic translocation can indicate epithelial-mesenchymal transition due to the activation of the canonical Wnt/β-catenin signaling pathway [1]. In hepatocellular carcinoma, VSIG1 may be involved in the lineage shift to gastric-type HCC, and the E-cadherin/VSIG1 complex may help suppress tumor growth [6]. In colon cancer, VSIG1 restricts the development of pro-metastatic states, in part by inhibiting YAP/TAZ-TEAD signaling [2]. A study on VSIG1 knockout (KO) mice found that VSIG1 may not play essential roles in spermatogenesis and normal testis development, function, and maintenance in mice [3].
In conclusion, Vsig1 has important functions in the proper differentiation of glandular gastric epithelia and in testis cell interactions. In cancer, its expression and translocation are associated with disease progression in gastric and hepatocellular carcinomas, and it has an anti-metastatic role in colon cancer. The Vsig1 KO mouse model has been valuable in revealing its dispensability in spermatogenesis and male fertility in mice, contributing to our understanding of its role in specific biological processes and disease conditions.
References：
1. Satala, Catalin-Bogdan, Jung, Ioan, Kovacs, Zsolt, Patrichi, Andrei-Ionut, Gurzu, Simona. 2022. V-set and immunoglobulin domain containing 1 (VSIG1) as an emerging target for epithelial-mesenchymal transition of gastric cancer. In Scientific reports, 12, 16241. doi:10.1038/s41598-022-19883-1. https://pubmed.ncbi.nlm.nih.gov/36171238/
2. Bernal, Carolina, Silvano, Marianna, Tapponnier, Yann, Angulo, Cecilia, Ruiz i Altaba, Ariel. . Functional Pro-metastatic Heterogeneity Revealed by Spiked-scRNAseq Is Shaped by Cancer Cell Interactions and Restricted by VSIG1. In Cell reports, 33, 108372. doi:10.1016/j.celrep.2020.108372. https://pubmed.ncbi.nlm.nih.gov/33176137/
3. Jung, Yena, Bang, Hyewon, Kim, Young-Hyun, Kim, Sun-Uk, Kim, Ekyune. 2021. V-Set and Immunoglobulin Domain-Containing 1 (VSIG1), Predominantly Expressed in Testicular Germ Cells, Is Dispensable for Spermatogenesis and Male Fertility in Mice. In Animals : an open access journal from MDPI, 11, . doi:10.3390/ani11041037. https://pubmed.ncbi.nlm.nih.gov/33916888/
4. Oidovsambuu, Odgerel, Nyamsuren, Gunsmaa, Liu, Shuai, Engel, Wolfgang, Adham, Ibrahim M. 2011. Adhesion protein VSIG1 is required for the proper differentiation of glandular gastric epithelia. In PloS one, 6, e25908. doi:10.1371/journal.pone.0025908. https://pubmed.ncbi.nlm.nih.gov/21991385/
5. Chen, Yibing, Pan, Ke, Li, Shengping, He, Jia, Li, Qiao. 2011. Decreased expression of V-set and immunoglobulin domain containing 1 (VSIG1) is associated with poor prognosis in primary gastric cancer. In Journal of surgical oncology, 106, 286-93. doi:10.1002/jso.22150. https://pubmed.ncbi.nlm.nih.gov/22095633/
6. Gurzu, Simona, Sugimura, Haruhiko, Szederjesi, Janos, Fodor, Decebal, Jung, Ioan. 2021. Interaction between cadherins, vimentin, and V-set and immunoglobulin domain containing 1 in gastric-type hepatocellular carcinoma. In Histochemistry and cell biology, 156, 377-390. doi:10.1007/s00418-021-02006-8. https://pubmed.ncbi.nlm.nih.gov/34170400/
7. Kim, Ekyune, Lee, Youngjeon, Kim, Ji-Su, Hong, Yonggeun, Chang, Kyu-Tae. 2010. Extracellular domain of V-set and immunoglobulin domain containing 1 (VSIG1) interacts with sertoli cell membrane protein, while its PDZ-binding motif forms a complex with ZO-1. In Molecules and cells, 30, 443-8. doi:10.1007/s10059-010-0138-4. https://pubmed.ncbi.nlm.nih.gov/20957455/
품질 관리 기준
정자 검사
동결 보존 전: 정자 농도 측정 및 정자 생존율 평가.
동결 보존 후: 각 배치에서 동결 보존된 정자 바이알 1개를 선택하여 체외수정(in vitro fertilization)에 사용합니다.
Environmental Standards:
SPFAvailable Region:
GlobalSource:
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