Dgkg-flox Mouse
Common Name
Dgkg-flox
제품 ID
S-CKO-18954
Backgroud
C57BL/6JCya
품종 계통계통 ID
CKOCMP-110197-Dgkg-B6J-VB
상태
이 마우스 계통을 논문에서 사용할 경우, “Dgkg-flox Mouse (카탈로그 번호 S-CKO-18954)은 Cyagen에서 구입하였습니다.”라고 명시해 주시기 바랍니다.
구매 가능한 제품 종류
연령
Genotype
성별
수량
표준 제공 조건은 최소 3마리의 이형접합(heterozygous) 보균자를 보장합니다. 동형접합(homozygous) 보균자 및/또는 특정 성별에 대한 브리딩 서비스도 제공됩니다.
기본 정보
품종 계통
Dgkg-flox
품종 계통계통 ID
CKOCMP-110197-Dgkg-B6J-VB
유전자명
제품 ID
S-CKO-18954
유전자 별칭
90kDa, Dagk3, mKIAA4131, 2900055E17Rik, E430001K23Rik
배경
C57BL/6JCya
NCBI ID
변형 내용
Conditional knockout
염색체
Chr 16
Phenotype
Datasheet
적용 분야
--
품종 계통 설명
Ensembl 전사체 ID
ENSMUST00000089925
NCBI 전사체 ID
NM_138650
타겟 영역
Exon 4~5
유효 영역 크기
~1.5 kb
유전자 연구 개요
Dgkg, or diacylglycerol kinase gamma, is a key enzyme in glycerophospholipid metabolism and phosphatidylinositol signaling pathways [8]. It plays significant roles in various biological processes, and its study via genetic models can provide insights into its functions.
In hepatocellular carcinoma (HCC), endothelial Dgkg promotes tumor angiogenesis and immunosuppressive regulatory T-cell differentiation. Hypoxia-induced HIF-1α activates Dgkg transcription, and upregulated Dgkg promotes HCC progression through the ZEB2/TGF-β1 axis. Targeting endothelial Dgkg potentiates the efficiency of dual blockade of PD-1 and VEGFR-2 [1].
In Wagyu cattle, a deleterious homozygous missense variant in Dgkg is associated with hepatic fibrinogen storage disease [2]. In an epileptic patient, a heterozygous mutation in Dgkg was identified, and an induced pluripotent stem cell line was generated for studying epilepsy mechanisms [3].
In oral squamous cell carcinoma (OSCC), Dgkg is among genes in a Cox model that can predict patient prognosis, and histone lysine lactylation-induced Dgkg may be involved in OSCC drug therapy and tumor microenvironment [4].
In acute myeloid leukemia (AML), high Dgkg expression is associated with a more favorable prognosis [5].
In colorectal cancer, Dgkg promoter is hypermethylated, suppressing its expression. Ectopic expression of Dgkg suppresses cell migration and invasion, suggesting a tumor-suppressor role [6].
In GABAergic interneurons, Dgkg is dominantly expressed in somatostatin-expressing neurons, and its functional study indicates an alteration in neurite outgrowth [7].
In glioblastoma, a hypoxia-induced alternative splicing transcript Dgkg-Δ exon13 promotes glioblastoma proliferation and infiltration [9].
In conclusion, Dgkg is involved in multiple biological processes and diseases. Its study through various models, such as in disease-associated genetic variants or in vitro and in vivo functional studies, helps understand its role in tumor angiogenesis, immune evasion, neurological development, and other pathological and physiological conditions.
References：
1. Zhang, Liren, Xu, Jiali, Zhou, Suiqing, Li, Qing, Wang, Xuehao. 2023. Endothelial DGKG promotes tumor angiogenesis and immune evasion in hepatocellular carcinoma. In Journal of hepatology, 80, 82-98. doi:10.1016/j.jhep.2023.10.006. https://pubmed.ncbi.nlm.nih.gov/37838036/
2. Jacinto, Joana G P, Wohlsein, Peter, Häfliger, Irene M, Grünberg, Walter, Drögemüller, Cord. 2023. A missense variant in DGKG as a recessive functional variant for hepatic fibrinogen storage disease in Wagyu cattle. In Journal of veterinary internal medicine, 37, 2631-2637. doi:10.1111/jvim.16865. https://pubmed.ncbi.nlm.nih.gov/37681469/
3. Liu, Ya-Qing, Ling, Tiao-Wen, Wang, Hong-Yan, Song, Wen-Jun, Wang, Tian-Cheng. 2022. Generation of an integration-free induced pluripotent stem cell line (LZUSHI001-A) from an epileptic patient with DGKG mutation. In Stem cell research, 61, 102768. doi:10.1016/j.scr.2022.102768. https://pubmed.ncbi.nlm.nih.gov/35421845/
4. Huang, Guangzhao, Chen, Su, He, Jialu, Guo, Zhiyong, Li, Chunjie. 2024. Histone Lysine Lactylation (Kla)-induced BCAM Promotes OSCC Progression and Cis-Platinum Resistance. In Oral diseases, 31, 1116-1132. doi:10.1111/odi.15179. https://pubmed.ncbi.nlm.nih.gov/39503345/
5. Gravina, Teresa, Boggio, Chiara Maria Teresa, Gorla, Elisa, Corà, Davide, Baldanzi, Gianluca. 2023. Role of Diacylglycerol Kinases in Acute Myeloid Leukemia. In Biomedicines, 11, . doi:10.3390/biomedicines11071877. https://pubmed.ncbi.nlm.nih.gov/37509516/
6. Kai, Masahiro, Yamamoto, Eiichiro, Sato, Akiko, Toyota, Minoru, Suzuki, Hiromu. 2017. Epigenetic silencing of diacylglycerol kinase gamma in colorectal cancer. In Molecular carcinogenesis, 56, 1743-1752. doi:10.1002/mc.22631. https://pubmed.ncbi.nlm.nih.gov/28218473/
7. Fukumoto, Keita, Tamada, Kota, Toya, Tsuyoshi, Yanagawa, Yuchio, Takumi, Toru. 2017. Identification of genes regulating GABAergic interneuron maturation. In Neuroscience research, 134, 18-29. doi:10.1016/j.neures.2017.11.010. https://pubmed.ncbi.nlm.nih.gov/29203264/
8. Taniguchi-Ponciano, Keiko, Hinojosa-Alvarez, Silvia, Hernandez-Perez, Jesus, Marrero-Rodriguez, Daniel, Mercado, Moises. 2024. Longitudinal multiomics analysis of aggressive pituitary neuroendocrine tumors: comparing primary and recurrent tumors from the same patient, reveals genomic stability and heterogeneous transcriptomic profiles with alterations in metabolic pathways. In Acta neuropathologica communications, 12, 142. doi:10.1186/s40478-024-01796-x. https://pubmed.ncbi.nlm.nih.gov/39217365/
9. Yang, Ming, Chu, Liangzhao, Lin, Shukai, Han, Feng, Liu, Jian. 2025. The alternatively spliced diacylglycerol kinase gamma-Δ exon13 transcript generated under hypoxia promotes glioblastoma progression. In Oncology research, 33, 1189-1198. doi:10.32604/or.2024.055102. https://pubmed.ncbi.nlm.nih.gov/40296900/
품질 관리 기준
정자 검사
동결 보존 전: 정자 농도 측정 및 정자 생존율 평가.
동결 보존 후: 각 배치에서 동결 보존된 정자 바이알 1개를 선택하여 체외수정(in vitro fertilization)에 사용합니다.
Environmental Standards:
SPFAvailable Region:
GlobalSource:
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