Coq3-flox Mouse
Common Name
Coq3-flox
제품 ID
S-CKO-19129
Backgroud
C57BL/6JCya
품종 계통계통 ID
CKOCMP-230027-Coq3-B6J-VB
상태
이 마우스 계통을 논문에서 사용할 경우, “Coq3-flox Mouse (카탈로그 번호 S-CKO-19129)은 Cyagen에서 구입하였습니다.”라고 명시해 주시기 바랍니다.
구매 가능한 제품 종류
연령
Genotype
성별
수량
표준 제공 조건은 최소 3마리의 이형접합(heterozygous) 보균자를 보장합니다. 동형접합(homozygous) 보균자 및/또는 특정 성별에 대한 브리딩 서비스도 제공됩니다.
기본 정보
품종 계통
Coq3-flox
품종 계통계통 ID
CKOCMP-230027-Coq3-B6J-VB
유전자명
제품 ID
S-CKO-19129
유전자 별칭
4732433J24
배경
C57BL/6JCya
NCBI ID
변형 내용
Conditional knockout
염색체
Chr 4
Phenotype
Datasheet
적용 분야
--
품종 계통 설명
Ensembl 전사체 ID
ENSMUST00000029909
NCBI 전사체 ID
NM_172687
타겟 영역
Exon 4~5
유효 영역 크기
~2.0 kb
유전자 연구 개요
Coq3, a gene encoding 3,4-dihydroxy-5-polyprenylbenzoate methyltransferase, is crucial for coenzyme Q (Q) biosynthesis [4,5,7,9]. Q is a redox-active lipid essential for aerobic respiration in eukaryotes, and Coq3-catalyzed O-methylation steps are key in its biosynthetic pathway [1,2,5,6,8,9]. Coq3 is part of a polypeptide complex in yeast mitochondria for Q biosynthesis, with Coq3 and Coq4 co-migrating as high-molecular-mass complexes [1]. It is also a membrane-binding protein, with human and yeast Coq3 preferring liposomes enriched with cardiolipin, a mitochondrial membrane lipid [2].
In Coq3-related functional studies, Rtn4ip1-knockout myoblasts showed decreased CoQ9 levels, suggesting that RTN4IP1, an NADPH oxidoreductase, is essential for CoQ biosynthesis by regulating Coq3 O-methylation activity [3]. In yeast, Coq3 null mutants can be rescued by expressing the human Coq3 construct or the E. coli UbiG polypeptide with a mitochondrial leader sequence, restoring Q biosynthesis and growth on non-fermentable carbon sources [5,6].
In conclusion, Coq3 is essential for coenzyme Q biosynthesis, playing a key role in aerobic respiration. Functional studies, including knockout models in myoblasts and yeast mutants, have revealed its role in CoQ synthesis. Understanding Coq3 can provide insights into mitochondrial function and potentially related disease conditions associated with Q deficiencies [3,5,6].
References：
1. Marbois, Beth, Gin, Peter, Faull, Kym F, Shepherd, Jennifer N, Clarke, Catherine F. 2005. Coq3 and Coq4 define a polypeptide complex in yeast mitochondria for the biosynthesis of coenzyme Q. In The Journal of biological chemistry, 280, 20231-8. doi:. https://pubmed.ncbi.nlm.nih.gov/15792955/
2. Zhu, Yuwei, Wu, Bo, Zhang, Xu, Wang, Junfeng, Teng, Maikun. 2015. Structural and biochemical studies reveal UbiG/Coq3 as a class of novel membrane-binding proteins. In The Biochemical journal, 470, 105-14. doi:10.1042/BJ20150329. https://pubmed.ncbi.nlm.nih.gov/26251450/
3. Park, Isaac, Kim, Kwang-Eun, Kim, Jeesoo, Suh, Jae Myoung, Rhee, Hyun-Woo. 2023. Mitochondrial matrix RTN4IP1/OPA10 is an oxidoreductase for coenzyme Q synthesis. In Nature chemical biology, 20, 221-233. doi:10.1038/s41589-023-01452-w. https://pubmed.ncbi.nlm.nih.gov/37884807/
4. Marbois, B N, Xia, Y R, Lusis, A J, Clarke, C F. . Ubiquinone biosynthesis in eukaryotic cells: tissue distribution of mRNA encoding 3,4-dihydroxy-5-polyprenylbenzoate methyltransferase in the rat and mapping of the COQ3 gene to mouse chromosome 4. In Archives of biochemistry and biophysics, 313, 83-8. doi:. https://pubmed.ncbi.nlm.nih.gov/8053692/
5. Jonassen, T, Clarke, C F. . Isolation and functional expression of human COQ3, a gene encoding a methyltransferase required for ubiquinone biosynthesis. In The Journal of biological chemistry, 275, 12381-7. doi:. https://pubmed.ncbi.nlm.nih.gov/10777520/
6. Hsu, A Y, Poon, W W, Shepherd, J A, Myles, D C, Clarke, C F. . Complementation of coq3 mutant yeast by mitochondrial targeting of the Escherichia coli UbiG polypeptide: evidence that UbiG catalyzes both O-methylation steps in ubiquinone biosynthesis. In Biochemistry, 35, 9797-806. doi:. https://pubmed.ncbi.nlm.nih.gov/8703953/
7. Clarke, C F, Williams, W, Teruya, J H. . Ubiquinone biosynthesis in Saccharomyces cerevisiae. Isolation and sequence of COQ3, the 3,4-dihydroxy-5-hexaprenylbenzoate methyltransferase gene. In The Journal of biological chemistry, 266, 16636-44. doi:. https://pubmed.ncbi.nlm.nih.gov/1885593/
8. Poon, W W, Barkovich, R J, Hsu, A Y, Myles, D C, Clarke, C F. . Yeast and rat Coq3 and Escherichia coli UbiG polypeptides catalyze both O-methyltransferase steps in coenzyme Q biosynthesis. In The Journal of biological chemistry, 274, 21665-72. doi:. https://pubmed.ncbi.nlm.nih.gov/10419476/
9. Avelange-Macherel, M H, Joyard, J. . Cloning and functional expression of AtCOQ3, the Arabidopsis homologue of the yeast COQ3 gene, encoding a methyltransferase from plant mitochondria involved in ubiquinone biosynthesis. In The Plant journal : for cell and molecular biology, 14, 203-13. doi:. https://pubmed.ncbi.nlm.nih.gov/9628017/
품질 관리 기준
정자 검사
동결 보존 전: 정자 농도 측정 및 정자 생존율 평가.
동결 보존 후: 각 배치에서 동결 보존된 정자 바이알 1개를 선택하여 체외수정(in vitro fertilization)에 사용합니다.
Environmental Standards:
SPFAvailable Region:
GlobalSource:
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