Areg-KO Mouse
Common Name
Areg-KO
제품 ID
S-KO-01117
Backgroud
C57BL/6NCya
품종 계통계통 ID
KOCMP-11839-Areg-B6N-VA
상태
이 마우스 계통을 논문에서 사용할 경우, “Areg-KO Mouse (카탈로그 번호 S-KO-01117)은 Cyagen에서 구입하였습니다.”라고 명시해 주시기 바랍니다.
구매 가능한 제품 종류
연령
Genotype
성별
수량
표준 제공 조건은 최소 3마리의 이형접합(heterozygous) 보균자를 보장합니다. 동형접합(homozygous) 보균자 및/또는 특정 성별에 대한 브리딩 서비스도 제공됩니다.
기본 정보
품종 계통
Areg-KO
품종 계통계통 ID
KOCMP-11839-Areg-B6N-VA
유전자명
제품 ID
S-KO-01117
유전자 별칭
AR, Mcub, Sdgf
배경
C57BL/6NCya
NCBI ID
변형 내용
Conventional knockout
염색체
Chr 5
Phenotype
Datasheet
적용 분야
--
품종 계통 설명
Ensembl 전사체 ID
ENSMUST00000031325
NCBI 전사체 ID
NM_009704
타겟 영역
Exon 2~4
유효 영역 크기
~3.6 kb
유전자 연구 개요
Areg, also known as amphiregulin, is a member of the epidermal growth factor (EGF) family. It acts as an extracellular ligand for the EGF receptor (EGFR) and also functions as an intracellular signaling molecule. Areg is involved in multiple biological processes such as cell survival, tissue growth, and it has been linked to pathways like Hippo-YAP [1]. It plays a vital role in immunity, inflammation, and tissue repair, and is expressed in various cell types including epithelial, mesenchymal, and multiple leukocyte populations [5].
In psoriasis, IL-17A promotes keratinocyte proliferation through activating the YAP-AREG axis. Inhibiting this axis with IL-17A antagonist secukinumab suppresses the pathway in human psoriatic skin [1]. In esophageal squamous cell carcinoma, co-culture with cancer-associated fibroblasts upregulates AREG, which promotes cancer progression via EGFR-Erk/p38 MAPK signaling [2]. In melanoma, nuclear AREG affects a low-proliferative phenotype and contributes to drug resistance, and knocking down AREG makes resistant cells more sensitive to treatment [3]. In non-alcoholic steatohepatitis, Areg-producing regulatory T cells promote liver fibrosis and insulin resistance, and deleting Areg in these T cells reduces NASH-induced liver fibrosis [4]. In pancreatic cancer, AREG mediates epithelial-mesenchymal transition via the EGFR/ERK/NF-κB signaling pathway, and knocking down AREG inhibits cell migration, invasion, and tumor growth [6]. Also, miR-33a-3p can regulate METTL3-mediated AREG stability to inhibit pancreatic cancer invasion and metastasis [7].
In conclusion, Areg is a multifunctional molecule involved in various biological processes and disease conditions. Gene-knockout or conditional-knockout mouse models and other loss-of-function experiments have revealed its role in diseases like psoriasis, cancer, and non-alcoholic steatohepatitis. Understanding Areg's functions through these models provides insights into disease mechanisms and potential therapeutic targets.
References:
1. Yu, Zengyang, Yu, Qian, Xu, Hui, Guo, Chunyuan, Shi, Yuling. 2022. IL-17A Promotes Psoriasis-Associated Keratinocyte Proliferation through ACT1-Dependent Activation of YAP-AREG Axis. In The Journal of investigative dermatology, 142, 2343-2352. doi:10.1016/j.jid.2022.02.016. https://pubmed.ncbi.nlm.nih.gov/35304250/
2. Nakanishi, Takashi, Koma, Yu-Ichiro, Miyako, Shoji, Yokozaki, Hiroshi, Kakeji, Yoshihiro. 2024. AREG Upregulation in Cancer Cells via Direct Interaction with Cancer-Associated Fibroblasts Promotes Esophageal Squamous Cell Carcinoma Progression Through EGFR-Erk/p38 MAPK Signaling. In Cells, 13, . doi:10.3390/cells13201733. https://pubmed.ncbi.nlm.nih.gov/39451251/
3. Seefried, Felix, Haller, Lucia, Fukuda, Shinji, Bosserhoff, Anja Katrin, Kuphal, Silke. 2022. Nuclear AREG affects a low-proliferative phenotype and contributes to drug resistance of melanoma. In International journal of cancer, 151, 2244-2264. doi:10.1002/ijc.34254. https://pubmed.ncbi.nlm.nih.gov/36054710/
4. Savage, Thomas M, Fortson, Katherine T, de Los Santos-Alexis, Kenia, Schwabe, Robert F, Arpaia, Nicholas. 2024. Amphiregulin from regulatory T cells promotes liver fibrosis and insulin resistance in non-alcoholic steatohepatitis. In Immunity, 57, 303-318.e6. doi:10.1016/j.immuni.2024.01.009. https://pubmed.ncbi.nlm.nih.gov/38309273/
5. Zaiss, Dietmar M W, Gause, William C, Osborne, Lisa C, Artis, David. . Emerging functions of amphiregulin in orchestrating immunity, inflammation, and tissue repair. In Immunity, 42, 216-226. doi:10.1016/j.immuni.2015.01.020. https://pubmed.ncbi.nlm.nih.gov/25692699/
6. Wang, Li, Wang, Lili, Zhang, Hui, Wu, Huanwen, Liang, Zhiyong. 2020. AREG mediates the epithelial‑mesenchymal transition in pancreatic cancer cells via the EGFR/ERK/NF‑κB signalling pathway. In Oncology reports, 43, 1558-1568. doi:10.3892/or.2020.7523. https://pubmed.ncbi.nlm.nih.gov/32323797/
7. Su, Xiaowen, Lai, Tiantian, Tao, Yue, Mao, Yong, Hu, Hao. 2023. miR-33a-3p regulates METTL3-mediated AREG stability and alters EMT to inhibit pancreatic cancer invasion and metastasis. In Scientific reports, 13, 13587. doi:10.1038/s41598-023-39506-7. https://pubmed.ncbi.nlm.nih.gov/37604948/
품질 관리 기준
정자 검사
동결 보존 전: 정자 농도 측정 및 정자 생존율 평가.
동결 보존 후: 각 배치에서 동결 보존된 정자 바이알 1개를 선택하여 체외수정(in vitro fertilization)에 사용합니다.
Environmental Standards:
SPFAvailable Region:
GlobalSource:
Cyagen문의하기
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