Prkcb-KO Mouse

Prkcb, or Protein kinase C beta, is a member of the classical PRKCs. It plays a role in negatively modulating the mitochondrial energy status and inhibiting autophagy, suggesting its importance in the regulation of autophagy [6]. It is also involved in pathways such as T cell receptor-NF-κB signaling and T cell-related pathways [5].

In lung adenocarcinoma (LUAD), Prkcb is relevant to prognosis through methylation and immune infiltration, being lowly expressed in LUAD patients [1]. In Ewing sarcoma, gene array analyses showed significant overexpression of Prkcb, and its immunohistochemistry is sensitive and specific in detecting EWSR1 rearranged cases, making it a reliable diagnostic antibody [2]. In intestinal ischemia/reperfusion, a circRNA transcribed from the Prkcb gene, circ-Prkcb, acts as an endogenous miR-339-5p sponge to regulate p66Shc expression, which is crucial for oxidative stress regulation [3]. In the context of periodontitis and osteoporosis, PRKCB is one of the key genes involved in neutrophil extracellular trap formation and MAPK signaling pathway [4].

In summary, Prkcb is involved in multiple biological processes and disease conditions. Its role in autophagy regulation, as well as its associations with various cancers, ischemia/reperfusion injury, and bone-related diseases, have been revealed through different studies. The understanding of Prkcb contributes to a better comprehension of these biological processes and disease mechanisms, potentially guiding future diagnostic and therapeutic strategies.

References:

1. Wang, Jinjie, Shi, Muqi, Zhang, Haijian, Zhou, Youlang, Shi, Jiahai. 2022. PRKCB is relevant to prognosis of lung adenocarcinoma through methylation and immune infiltration. In Thoracic cancer, 13, 1837-1849. doi:10.1111/1759-7714.14466. https://pubmed.ncbi.nlm.nih.gov/35567329/

2. Zota, Victor, Siegal, Gene P, Kelly, David, Magliocco, Anthony, Bui, Marilyn M. 2022. Validation of PRKCB Immunohistochemistry as a Biomarker for the Diagnosis of Ewing Sarcoma. In Fetal and pediatric pathology, 42, 241-252. doi:10.1080/15513815.2022.2117579. https://pubmed.ncbi.nlm.nih.gov/36062956/

3. Feng, Dongcheng, Wang, Zhecheng, Zhao, Yan, Yao, Jihong, Tian, Xiaofeng. 2020. circ-PRKCB acts as a ceRNA to regulate p66Shc-mediated oxidative stress in intestinal ischemia/reperfusion. In Theranostics, 10, 10680-10696. doi:10.7150/thno.44250. https://pubmed.ncbi.nlm.nih.gov/32929374/

4. Liu, Jia, Zhang, Ding, Cao, Yu, Ye, Surong, Yang, Luyi. 2022. Screening of crosstalk and pyroptosis-related genes linking periodontitis and osteoporosis based on bioinformatics and machine learning. In Frontiers in immunology, 13, 955441. doi:10.3389/fimmu.2022.955441. https://pubmed.ncbi.nlm.nih.gov/35990678/

5. Kataoka, Keisuke, Nagata, Yasunobu, Kitanaka, Akira, Shimoda, Kazuya, Ogawa, Seishi. 2015. Integrated molecular analysis of adult T cell leukemia/lymphoma. In Nature genetics, 47, 1304-15. doi:10.1038/ng.3415. https://pubmed.ncbi.nlm.nih.gov/26437031/

6. Patergnani, Simone, Marchi, Saverio, Rimessi, Alessandro, Mehta, Kamal D, Pinton, Paolo. 2013. PRKCB/protein kinase C, beta and the mitochondrial axis as key regulators of autophagy. In Autophagy, 9, 1367-85. doi:10.4161/auto.25239. https://pubmed.ncbi.nlm.nih.gov/23778835/