Prpf4b-KO Mouse
Common Name
Prpf4b-KO
제품 ID
S-KO-03801
Backgroud
C57BL/6JCya
품종 계통계통 ID
KOCMP-19134-Prpf4b-B6J-VB
상태
이 마우스 계통을 논문에서 사용할 경우, “Prpf4b-KO Mouse (카탈로그 번호 S-KO-03801)은 Cyagen에서 구입하였습니다.”라고 명시해 주시기 바랍니다.
구매 가능한 제품 종류
연령
Genotype
성별
수량
표준 제공 조건은 최소 3마리의 이형접합(heterozygous) 보균자를 보장합니다. 동형접합(homozygous) 보균자 및/또는 특정 성별에 대한 브리딩 서비스도 제공됩니다.
기본 정보
품종 계통
Prpf4b-KO
품종 계통계통 ID
KOCMP-19134-Prpf4b-B6J-VB
유전자명
제품 ID
S-KO-03801
유전자 별칭
Prp4, Prpk, Prp4k, cbp143, 2610037H07
배경
C57BL/6JCya
NCBI ID
변형 내용
Conventional knockout
염색체
Chr 13
Phenotype
Datasheet
적용 분야
--
품종 계통 설명
Ensembl 전사체 ID
ENSMUST00000077853
NCBI 전사체 ID
NM_013830
타겟 영역
Exon 4~5
유효 영역 크기
~2.6 kb
유전자 연구 개요
Prpf4b, also known as PRP4K, is an essential kinase first identified in fission yeast and evolutionarily conserved. It is a pre-mRNA splicing regulator, phosphorylating spliceosomal components like PRPF6 and PRPF31 during spliceosome B complex formation. It is also involved in pathways such as transcription regulation and the spindle assembly checkpoint, playing an important role in maintaining chromosome stability [4,5].
In cancer research, several studies have implicated Prpf4b. In colon cancer, the phosphorylation level of PRPF4BS431p was significantly correlated with the overall survival of patients, suggesting a role in the disease's prognosis [1]. In lung adenocarcinoma, miR-224 was found to down-regulate PRPF4B, and this miRNA-mRNA pair was associated with lymph node metastasis and prognosis [2]. In hepatocellular carcinoma, miR-371-5p down-regulates PRPF4B, facilitating the G1/S transition and promoting tumor growth [3]. In triple-negative breast cancer, PRPF4B is essential for cancer cell migration and metastasis, and its depletion down-regulates genes in focal adhesion and ECM-interaction pathways [6]. In breast and ovarian cancer, PRP4K (PRPF4B) is a HER2-regulated modifier of taxane sensitivity, with low PRP4K levels correlating with taxane resistance [7]. Also, PRP4K is negatively regulated during epithelial-to-mesenchymal transition, and its depletion in normal mammary cells can increase cell invasion [8].
In conclusion, Prpf4b is crucial for pre-mRNA splicing and participates in multiple cellular pathways. Model-based research, especially in cancer, has shown its significance in tumorigenesis, metastasis, and treatment response. Understanding Prpf4b functions provides insights into disease mechanisms and potential therapeutic targets for various cancers.
References:
1. Gao, Li, Lu, Ying, Chen, Hai-Ning, Zhou, Zong-Guang, Dai, Lunzhi. 2023. Deciphering the Clinical Significance and Kinase Functions of GSK3α in Colon Cancer by Proteomics and Phosphoproteomics. In Molecular & cellular proteomics : MCP, 22, 100545. doi:10.1016/j.mcpro.2023.100545. https://pubmed.ncbi.nlm.nih.gov/37031867/
2. Wang, Yan, Shang, Shengtao, Yu, Kun, Ma, Wenduan, Zhao, Wei. 2020. miR-224, miR-147b and miR-31 associated with lymph node metastasis and prognosis for lung adenocarcinoma by regulating PRPF4B, WDR82 or NR3C2. In PeerJ, 8, e9704. doi:10.7717/peerj.9704. https://pubmed.ncbi.nlm.nih.gov/33282547/
3. Liu, Rui-Yan, Diao, Cai-Feng, Zhang, Yi, Liu, Min, Tang, Hua. 2013. miR-371-5p down-regulates pre mRNA processing factor 4 homolog B (PRPF4B) and facilitates the G1/S transition in human hepatocellular carcinoma cells. In Cancer letters, 335, 351-60. doi:10.1016/j.canlet.2013.02.045. https://pubmed.ncbi.nlm.nih.gov/23466643/
4. Corkery, Dale P, Holly, Alice C, Lahsaee, Sara, Dellaire, Graham. 2015. Connecting the speckles: Splicing kinases and their role in tumorigenesis and treatment response. In Nucleus (Austin, Tex.), 6, 279-88. doi:10.1080/19491034.2015.1062194. https://pubmed.ncbi.nlm.nih.gov/26098145/
5. Habib, Elias B, Mathavarajah, Sabateeshan, Dellaire, Graham. 2022. Tinker, Tailor, Tumour Suppressor: The Many Functions of PRP4K. In Frontiers in genetics, 13, 839963. doi:10.3389/fgene.2022.839963. https://pubmed.ncbi.nlm.nih.gov/35281802/
6. Koedoot, Esmee, Fokkelman, Michiel, Rogkoti, Vasiliki-Maria, Martens, John W M, van de Water, Bob. 2019. Uncovering the signaling landscape controlling breast cancer cell migration identifies novel metastasis driver genes. In Nature communications, 10, 2983. doi:10.1038/s41467-019-11020-3. https://pubmed.ncbi.nlm.nih.gov/31278301/
7. Corkery, Dale P, Le Page, Cécile, Meunier, Liliane, Mes-Masson, Anne-Marie, Dellaire, Graham. . PRP4K is a HER2-regulated modifier of taxane sensitivity. In Cell cycle (Georgetown, Tex.), 14, 1059-69. doi:10.1080/15384101.2015.1007775. https://pubmed.ncbi.nlm.nih.gov/25602630/
8. Clarke, Livia E, Cook, Allyson, Mathavarajah, Sabateeshan, Lewis, Stephen M, Dellaire, Graham. . Haploinsufficient tumor suppressor PRP4K is negatively regulated during epithelial-to-mesenchymal transition. In FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 35, e22001. doi:10.1096/fj.202001063R. https://pubmed.ncbi.nlm.nih.gov/34674320/
품질 관리 기준
정자 검사
동결 보존 전: 정자 농도 측정 및 정자 생존율 평가.
동결 보존 후: 각 배치에서 동결 보존된 정자 바이알 1개를 선택하여 체외수정(in vitro fertilization)에 사용합니다.
Environmental Standards:
SPFAvailable Region:
GlobalSource:
Cyagen문의하기
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