Tcf3-KO Mouse
Common Name
Tcf3-KO
제품 ID
S-KO-05007
Backgroud
C57BL/6JCya
품종 계통계통 ID
KOCMP-21423-Tcf3-B6J-VA
상태
이 마우스 계통을 논문에서 사용할 경우, “Tcf3-KO Mouse (카탈로그 번호 S-KO-05007)은 Cyagen에서 구입하였습니다.”라고 명시해 주시기 바랍니다.
구매 가능한 제품 종류
연령
Genotype
성별
수량
표준 제공 조건은 최소 3마리의 이형접합(heterozygous) 보균자를 보장합니다. 동형접합(homozygous) 보균자 및/또는 특정 성별에 대한 브리딩 서비스도 제공됩니다.
기본 정보
품종 계통
Tcf3-KO
품종 계통계통 ID
KOCMP-21423-Tcf3-B6J-VA
유전자명
제품 ID
S-KO-05007
유전자 별칭
A1, E12, E2A, E47, KA1, ME2, ALF2, Pan1, Pan2, VDIR, TCF-3, Tcfe2a, E12/E47, bHLHb21
배경
C57BL/6JCya
NCBI ID
변형 내용
Conventional knockout
염색체
Chr 10
Phenotype
Datasheet
적용 분야
--
품종 계통 설명
Ensembl 전사체 ID
ENSMUST00000020377
NCBI 전사체 ID
NM_001164148
타겟 영역
Exon 3
유효 영역 크기
~0.8 kb
유전자 연구 개요
Tcf3, also known as transcription factor 3 or E2A, is a pivotal transcription factor. It contributes to early lymphocyte differentiation, is involved in the B-cell receptor (BCR) signaling pathway, and plays a role in embryogenesis [2,6,9]. Tcf3 is also a member of the TCF/LEF family, with impacts on tumorigenesis, and is associated with the Wnt signaling pathway [7,8]. Genetic models, such as KO mouse models, are valuable for studying its functions.
In mice, Tcf3 haploinsufficiency leads to a reduction in circulating B cells but overall normal humoral immune responses, indicating its role in lymphocyte development [2]. In mouse oocytes and early embryos, Tcf3 is identified as a key folliculogenesis regulator, with its deficiency impairing activation of key oocyte genes [1]. In CD8+ T-cell exhaustion, Id2 disrupts the assembly of the Tcf3-LSD1 complex, modulating chromatin accessibility at the Slamf6 promoter and influencing the generation of progenitor exhausted (Texprog) cells [3].
In conclusion, Tcf3 is crucial for lymphocyte development, folliculogenesis, and CD8+ T-cell exhaustion. The use of KO mouse models has significantly advanced our understanding of Tcf3's role in immunodeficiency, ovarian function, and tumor immune evasion. Additionally, Tcf3's associations with cancer, such as in leukemia and glioma, highlight its importance in disease-related research [1,2,3,4,5,7,8].
References:
1. Liu, Bofeng, He, Yuanlin, Wu, Xiaotong, Li, Jing, Xie, Wei. 2024. Mapping putative enhancers in mouse oocytes and early embryos reveals TCF3/12 as key folliculogenesis regulators. In Nature cell biology, 26, 962-974. doi:10.1038/s41556-024-01422-x. https://pubmed.ncbi.nlm.nih.gov/38839978/
2. Boast, Brigette, Goel, Shubham, González-Granado, Luis I, Kuehn, Hye Sun, Rosenzweig, Sergio D. 2023. TCF3 haploinsufficiency defined by immune, clinical, gene-dosage, and murine studies. In The Journal of allergy and clinical immunology, 152, 736-747. doi:10.1016/j.jaci.2023.05.017. https://pubmed.ncbi.nlm.nih.gov/37277074/
3. Li, Yiming, Han, Mingwei, Wei, Haolin, Zhu, Ping, Chen, Liang. 2024. Id2 epigenetically controls CD8+ T-cell exhaustion by disrupting the assembly of the Tcf3-LSD1 complex. In Cellular & molecular immunology, 21, 292-308. doi:10.1038/s41423-023-01118-6. https://pubmed.ncbi.nlm.nih.gov/38287103/
4. Salim, Mustafa, Heldt, Frederik, Thomay, Kathrin, Schlegelberger, Brigitte, Göhring, Gudrun. 2021. Cryptic TCF3 fusions in childhood leukemia: Detection by RNA sequencing. In Genes, chromosomes & cancer, 61, 22-26. doi:10.1002/gcc.22998. https://pubmed.ncbi.nlm.nih.gov/34460133/
5. Zerkalenkova, Elena, Menchits, Yaroslav, Borkovskaia, Alexandra, Karachunskii, Alexander, Olshanskaya, Yulia. 2023. TCF3 gene rearrangements in pediatric B-cell acute lymphoblastic leukemia-A single center experience. In International journal of laboratory hematology, 45, 533-540. doi:10.1111/ijlh.14072. https://pubmed.ncbi.nlm.nih.gov/37058324/
6. Wilke, Anne C, Doebele, Carmen, Zindel, Alena, Zenz, Thorsten, Oellerich, Thomas. . SHMT2 inhibition disrupts the TCF3 transcriptional survival program in Burkitt lymphoma. In Blood, 139, 538-553. doi:10.1182/blood.2021012081. https://pubmed.ncbi.nlm.nih.gov/34624079/
7. Nie, Huiling, Yu, Yang, Zhou, Siqi, Wei, Bingbing, Qin, Xiaojian. . TCF3 as a multidimensional biomarker: oncogenicity, genomic alterations, and immune landscape in pan-cancer analysis. In Acta biochimica et biophysica Sinica, 57, 195-208. doi:10.3724/abbs.2024126. https://pubmed.ncbi.nlm.nih.gov/39205642/
8. Zeng, Wei, Jiang, Haixiao, Wang, Ying, Wang, Cunzu, Yu, Bo. 2022. TCF3 Induces DNMT1 Expression to Regulate Wnt Signaling Pathway in Glioma. In Neurotoxicity research, 40, 721-732. doi:10.1007/s12640-022-00510-w. https://pubmed.ncbi.nlm.nih.gov/35446002/
9. Wei, Xiao-Wei, Liu, Xue-Qing, Zhang, Yu-Chen, Lin, Yi, Tian, Fu-Ju. 2022. TCF3 regulates human endometrial stromal cell proliferation and migration in RPL. In Reproduction (Cambridge, England), 163, 281-291. doi:10.1530/REP-21-0463. https://pubmed.ncbi.nlm.nih.gov/35239510/
품질 관리 기준
정자 검사
동결 보존 전: 정자 농도 측정 및 정자 생존율 평가.
동결 보존 후: 각 배치에서 동결 보존된 정자 바이알 1개를 선택하여 체외수정(in vitro fertilization)에 사용합니다.
Environmental Standards:
SPFAvailable Region:
GlobalSource:
Cyagen문의하기
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