Mboat4-KO Mouse
Common Name
Mboat4-KO
제품 ID
S-KO-06691
Backgroud
C57BL/6NCya
품종 계통계통 ID
KOCMP-234155-Mboat4-B6N-VA
상태
이 마우스 계통을 논문에서 사용할 경우, “Mboat4-KO Mouse (카탈로그 번호 S-KO-06691)은 Cyagen에서 구입하였습니다.”라고 명시해 주시기 바랍니다.
구매 가능한 제품 종류
연령
Genotype
성별
수량
표준 제공 조건은 최소 3마리의 이형접합(heterozygous) 보균자를 보장합니다. 동형접합(homozygous) 보균자 및/또는 특정 성별에 대한 브리딩 서비스도 제공됩니다.
기본 정보
품종 계통
Mboat4-KO
품종 계통계통 ID
KOCMP-234155-Mboat4-B6N-VA
유전자명
제품 ID
S-KO-06691
유전자 별칭
GOAT, Gm171
배경
C57BL/6NCya
NCBI ID
변형 내용
Conventional knockout
염색체
Chr 8
Phenotype
Datasheet
적용 분야
--
품종 계통 설명
Ensembl 전사체 ID
ENSMUST00000095345
NCBI 전사체 ID
NM_001126314
타겟 영역
Exon 2~3
유효 영역 크기
~4.1 kb
유전자 연구 개요
Mboat4, also known as ghrelin O-acyl transferase (GOAT), is a crucial enzyme. It catalyzes the post-translational octanoylation of Ser-3 residue of ghrelin, a 28-residue peptide hormone produced by stomach P/D1 cells. This modification is essential for ghrelin to bind to its receptor in target tissues. Ghrelin is involved in multiple physiological processes such as regulating food intake, growth hormone secretion from the pituitary, and inhibiting insulin secretion from the pancreas, highlighting the biological importance of Mboat4 in these associated pathways [1,6,7,8,9].
Some research indicates Mboat4 may be involved in lipid metabolism as gene-educational attainment interactions identified it as part of novel lipid loci, suggesting its role in adipose biology [2]. In addition, in Japanese patients with schizophrenia, the mRNA expression of Mboat4 was significantly increased, potentially reflecting the mechanisms of the disease [3]. Also, in silico studies identified pathogenic SNPs in Mboat4, suggesting it could be an anti-obesity target as it catalyzes ghrelin acylation which is related to prominent ghrelin activity [4]. In Alzheimer's disease patients, the methylation rate of Mboat4 CpG 2 was lower and its mRNA expression was higher, which may be a neuroprotective biomarker [5].
In conclusion, Mboat4 is essential for ghrelin activation and thus for various physiological functions related to metabolism and hormone regulation. Studies on Mboat4 in different disease conditions such as schizophrenia, Alzheimer's disease, and obesity-related research contribute to understanding its role in these pathologies, potentially providing new directions for therapeutic interventions.
References:
1. Murzinski, Emily S, Saha, Ishika, Ding, Hui, Tontonoz, Peter, Harran, Patrick G. 2021. In Search of Small Molecules That Selectively Inhibit MBOAT4. In Molecules (Basel, Switzerland), 26, . doi:10.3390/molecules26247599. https://pubmed.ncbi.nlm.nih.gov/34946685/
2. de las Fuentes, Lisa, Schwander, Karen L, Brown, Michael R, Rotter, Jerome I, Fornage, Myriam. 2023. Gene-educational attainment interactions in a multi-population genome-wide meta-analysis identify novel lipid loci. In Frontiers in genetics, 14, 1235337. doi:10.3389/fgene.2023.1235337. https://pubmed.ncbi.nlm.nih.gov/38028628/
3. Nakata, Shunsuke, Yoshino, Yuta, Okita, Mitsuo, Iga, Jun-Ichi, Ueno, Shu-Ichi. 2018. Differential expression of the ghrelin-related mRNAs GHS-R1a, GHS-R1b, and MBOAT4 in Japanese patients with schizophrenia. In Psychiatry research, 272, 334-339. doi:10.1016/j.psychres.2018.12.135. https://pubmed.ncbi.nlm.nih.gov/30597386/
4. Azmi, Muhammad Bilal, Sehgal, Sheikh Arslan, Asif, Uzma, Ahmed, Syed Danish Haseen, Qureshi, Shamim Akhtar. 2023. Genetic insights into obesity: in silico identification of pathogenic SNPs in MBOAT4 gene and their structural molecular dynamics consequences. In Journal of biomolecular structure & dynamics, 42, 13074-13090. doi:10.1080/07391102.2023.2274970. https://pubmed.ncbi.nlm.nih.gov/37921712/
5. Yoshino, Yuta, Funahashi, Yu, Nakata, Shunsuke, Iga, Jun-Ichi, Ueno, Shu-Ichi. 2018. Ghrelin cascade changes in the peripheral blood of Japanese patients with Alzheimer's disease. In Journal of psychiatric research, 107, 79-85. doi:10.1016/j.jpsychires.2018.10.011. https://pubmed.ncbi.nlm.nih.gov/30366284/
6. Sato, Takahiro, Ida, Takanori, Nakamura, Yuki, Kangawa, Kenji, Kojima, Masayasu. 2013. Physiological roles of ghrelin on obesity. In Obesity research & clinical practice, 8, e405-13. doi:10.1016/j.orcp.2013.10.002. https://pubmed.ncbi.nlm.nih.gov/25263830/
7. Taylor, Martin S, Hwang, Yousang, Hsiao, Po-Yuan, Boeke, Jef D, Cole, Philip A. . Ghrelin O-acyltransferase assays and inhibition. In Methods in enzymology, 514, 205-28. doi:10.1016/B978-0-12-381272-8.00013-1. https://pubmed.ncbi.nlm.nih.gov/22975055/
8. Abizaid, Alfonso, Hougland, James L. 2019. Ghrelin Signaling: GOAT and GHS-R1a Take a LEAP in Complexity. In Trends in endocrinology and metabolism: TEM, 31, 107-117. doi:10.1016/j.tem.2019.09.006. https://pubmed.ncbi.nlm.nih.gov/31636018/
9. Delhanty, P J D, van der Lely, A J. 2011. Ghrelin and glucose homeostasis. In Peptides, 32, 2309-18. doi:10.1016/j.peptides.2011.03.001. https://pubmed.ncbi.nlm.nih.gov/21396419/
품질 관리 기준
정자 검사
동결 보존 전: 정자 농도 측정 및 정자 생존율 평가.
동결 보존 후: 각 배치에서 동결 보존된 정자 바이알 1개를 선택하여 체외수정(in vitro fertilization)에 사용합니다.
Environmental Standards:
SPFAvailable Region:
GlobalSource:
Cyagen문의하기
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