Fgl1-KO Mouse
Common Name
Fgl1-KO
제품 ID
S-KO-06692
Backgroud
C57BL/6NCya
품종 계통계통 ID
KOCMP-234199-Fgl1-B6N-VA
상태
이 마우스 계통을 논문에서 사용할 경우, “Fgl1-KO Mouse (카탈로그 번호 S-KO-06692)은 Cyagen에서 구입하였습니다.”라고 명시해 주시기 바랍니다.
구매 가능한 제품 종류
연령
Genotype
성별
수량
표준 제공 조건은 최소 3마리의 이형접합(heterozygous) 보균자를 보장합니다. 동형접합(homozygous) 보균자 및/또는 특정 성별에 대한 브리딩 서비스도 제공됩니다.
기본 정보
품종 계통
Fgl1-KO
품종 계통계통 ID
KOCMP-234199-Fgl1-B6N-VA
유전자명
제품 ID
S-KO-06692
유전자 별칭
Mfire1
배경
C57BL/6NCya
NCBI ID
변형 내용
Conventional knockout
염색체
Chr 8
Phenotype
Datasheet
적용 분야
--
품종 계통 설명
Ensembl 전사체 ID
ENSMUST00000034003
NCBI 전사체 ID
NM_145594
타겟 영역
Exon 3~4
유효 영역 크기
~9.4 kb
유전자 연구 개요
Fgl1, or fibrinogen-like protein 1, is a protein secreted mainly by the liver. It is involved in multiple biological functions including regulating hepcidin and iron metabolism by antagonizing BMP signaling during anemia recovery in mice [5]. It also plays a crucial role in the immune system as a ligand of lymphocyte activation gene 3 (LAG3), and their interaction is associated with immune cell infiltration, proliferation, and secretion [1,2,6]. In addition, Fgl1 is related to cell proliferation and metabolism, and its expression is linked to various diseases, especially cancers [2].
In metastatic colorectal cancer, Fgl1 secreted from cancer cells and hepatocytes reduces T-cell infiltration, facilitating tumor progression. Disrupting the TAM-OTUD1-FGL1 axis, which upregulates FGL1 stability, inhibits metastatic tumor progression [3]. In non-small cell lung cancer (NSCLC), high FGL1 expression confers resistance to EGFR-TKI and gefitinib, and FGL1 knockdown enhances apoptosis induced by gefitinib [1,4]. Also, in NSCLC, Fgl1 promotes tumor cell proliferation, migration, and invasion through the KDM4A/STAT3 transcription mechanism, and its dynamic expression changes on circulating tumor cells can be a biomarker for predicting treatment efficacy [7]. In systemic lupus erythematosus (SLE) with liver damage, up-regulated Fgl1 from the injured liver, through the FGL1-LAG3 axis, impairs the suppressive function of regulatory T cells [8].
In conclusion, Fgl1 is a multifunctional protein involved in iron metabolism, immune regulation, and cancer progression. Mouse models, especially those disrupting relevant axes like the TAM-OTUD1-FGL1 axis in cancer, have revealed its role in disease development. These findings suggest that targeting Fgl1 could be a potential therapeutic strategy for various diseases, including cancers and SLE-related liver damage.
References:
1. Shi, An-Ping, Tang, Xi-Yang, Xiong, Yan-Lu, Ma, Nan, Zhao, Jin-Bo. 2022. Immune Checkpoint LAG3 and Its Ligand FGL1 in Cancer. In Frontiers in immunology, 12, 785091. doi:10.3389/fimmu.2021.785091. https://pubmed.ncbi.nlm.nih.gov/35111155/
2. Qian, Wenjing, Zhao, Mingfang, Wang, Ruoyu, Li, Heming. 2021. Fibrinogen-like protein 1 (FGL1): the next immune checkpoint target. In Journal of hematology & oncology, 14, 147. doi:10.1186/s13045-021-01161-8. https://pubmed.ncbi.nlm.nih.gov/34526102/
3. Li, Jia-Jun, Wang, Jin-Hong, Tian, Tian, Xu, Rui-Hua, Ju, Huai-Qiang. 2023. The liver microenvironment orchestrates FGL1-mediated immune escape and progression of metastatic colorectal cancer. In Nature communications, 14, 6690. doi:10.1038/s41467-023-42332-0. https://pubmed.ncbi.nlm.nih.gov/37872170/
4. Sun, Cuilan, Gao, Weiwei, Liu, Jiatao, Cheng, Hao, Hao, Jiqing. 2020. FGL1 regulates acquired resistance to Gefitinib by inhibiting apoptosis in non-small cell lung cancer. In Respiratory research, 21, 210. doi:10.1186/s12931-020-01477-y. https://pubmed.ncbi.nlm.nih.gov/32778129/
5. Sardo, Ugo, Perrier, Prunelle, Cormier, Kevin, Ganz, Tomas, Kautz, Léon. . The hepatokine FGL1 regulates hepcidin and iron metabolism during anemia in mice by antagonizing BMP signaling. In Blood, 143, 1282-1292. doi:10.1182/blood.2023022724. https://pubmed.ncbi.nlm.nih.gov/38232308/
6. Wang, Jun, Sanmamed, Miguel F, Datar, Ila, Schalper, Kurt, Chen, Lieping. 2018. Fibrinogen-like Protein 1 Is a Major Immune Inhibitory Ligand of LAG-3. In Cell, 176, 334-347.e12. doi:10.1016/j.cell.2018.11.010. https://pubmed.ncbi.nlm.nih.gov/30580966/
7. Liu, Tian Yao, Yan, Jin Shan, Li, Xin, Zhao, Yue, Zhao, Ming Fang. 2024. FGL1: a novel biomarker and target for non-small cell lung cancer, promoting tumor progression and metastasis through KDM4A/STAT3 transcription mechanism. In Journal of experimental & clinical cancer research : CR, 43, 213. doi:10.1186/s13046-024-03140-6. https://pubmed.ncbi.nlm.nih.gov/39085849/
8. Chen, Kang, Li, Xingyu, Shang, Yuqi, Ming, Siqi, Wu, Yongjian. 2023. FGL1-LAG3 axis impairs IL-10-Producing regulatory T cells associated with Systemic lupus erythematosus disease activity. In Heliyon, 9, e20806. doi:10.1016/j.heliyon.2023.e20806. https://pubmed.ncbi.nlm.nih.gov/37916085/
품질 관리 기준
정자 검사
동결 보존 전: 정자 농도 측정 및 정자 생존율 평가.
동결 보존 후: 각 배치에서 동결 보존된 정자 바이알 1개를 선택하여 체외수정(in vitro fertilization)에 사용합니다.
Environmental Standards:
SPFAvailable Region:
GlobalSource:
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