Ncbp1-KO Mouse
Common Name
Ncbp1-KO
제품 ID
S-KO-10093
Backgroud
C57BL/6JCya
품종 계통계통 ID
KOCMP-433702-Ncbp1-B6J-VA
상태
이 마우스 계통을 논문에서 사용할 경우, “Ncbp1-KO Mouse (카탈로그 번호 S-KO-10093)은 Cyagen에서 구입하였습니다.”라고 명시해 주시기 바랍니다.
구매 가능한 제품 종류
연령
Genotype
성별
수량
표준 제공 조건은 최소 3마리의 이형접합(heterozygous) 보균자를 보장합니다. 동형접합(homozygous) 보균자 및/또는 특정 성별에 대한 브리딩 서비스도 제공됩니다.
기본 정보
품종 계통
Ncbp1-KO
품종 계통계통 ID
KOCMP-433702-Ncbp1-B6J-VA
유전자명
제품 ID
S-KO-10093
유전자 별칭
CBP80
배경
C57BL/6JCya
NCBI ID
변형 내용
Conventional knockout
염색체
Chr 4
Phenotype
Datasheet
적용 분야
--
품종 계통 설명
Ensembl 전사체 ID
ENSMUST00000030014
NCBI 전사체 ID
NM_001033201
타겟 영역
Exon 2~8
유효 영역 크기
~9.1 kb
유전자 연구 개요
Ncbp1, also known as Nuclear cap-binding protein 1, is essential for capped RNA processing and intracellular localization. It forms the nuclear Cap-Binding Complex (CBC) with NCBP2 and an alternative CBC with NCBP3, playing roles in RNA fate decisions, such as transcription, splicing, polyadenylation, and mRNA export [5,6,8]. It is also involved in translation as part of the nuclear cap-binding protein complex-dependent translation machinery [9].
In disease-related research, NCBP1 shows diverse impacts. In diffuse large B-cell lymphoma (DLBCL), its elevated expression is associated with poor prognosis, and it promotes cell proliferation via the METTL3-mediated m6A modification of c-Myc [1]. In lung adenocarcinoma, NCBP1 overexpression promotes cancer cell growth, migration, etc., by up-regulating CUL4B [3]. In breast cancer, NCBP1 is identified as a prognostic and immune-associated biomarker, and knockdown of it suppresses malignancy in vitro [7]. In renal tubular epithelial cells, the IGF2BP3/NCBP1 complex inhibits senescence by regulating CDK6 mRNA stability [2]. Conversely, in epilepsy, NCBP1 is downregulated in the epileptic hippocampus, and its overexpression improves cognitive function by reducing oxidative stress, neuronal loss, and glial activation [4].
In conclusion, Ncbp1 is crucial for RNA-related processes and is implicated in multiple diseases. Studies on Ncbp1, including those using in vitro knockdown models, have provided insights into its roles in cancer, renal senescence, and epilepsy, potentially guiding the development of targeted therapies for these conditions.
References:
1. Meng, Sibo, Xia, Yuan, Li, Mingying, Sun, Tao, Ji, Chunyan. 2023. NCBP1 enhanced proliferation of DLBCL cells via METTL3-mediated m6A modification of c-Myc. In Scientific reports, 13, 8606. doi:10.1038/s41598-023-35777-2. https://pubmed.ncbi.nlm.nih.gov/37244946/
2. Li, Yaqin, Luo, Congwei, Cai, Yating, Wang, Hongsheng, Niu, Hongxin. 2024. IGF2BP3/NCBP1 complex inhibits renal tubular senescence through regulation of CDK6 mRNA stability. In Translational research : the journal of laboratory and clinical medicine, 273, 1-15. doi:10.1016/j.trsl.2024.06.004. https://pubmed.ncbi.nlm.nih.gov/38945255/
3. Zhang, Huijun, Wang, An, Tan, Yulong, Chen, Xiaofeng, He, Zelai. 2019. NCBP1 promotes the development of lung adenocarcinoma through up-regulation of CUL4B. In Journal of cellular and molecular medicine, 23, 6965-6977. doi:10.1111/jcmm.14581. https://pubmed.ncbi.nlm.nih.gov/31448526/
4. Gao, Xiaoying, You, Zhipeng, Huang, Cong, Qi, Sihua, Sun, Jiahang. 2023. NCBP1 Improves Cognitive Function in Mice by Reducing Oxidative Stress, Neuronal Loss, and Glial Activation After Status Epilepticus. In Molecular neurobiology, 60, 6676-6688. doi:10.1007/s12035-023-03497-3. https://pubmed.ncbi.nlm.nih.gov/37474884/
5. Dou, Yuhui, Barbosa, Isabelle, Jiang, Hua, LaCava, John, Jensen, Torben Heick. . NCBP3 positively impacts mRNA biogenesis. In Nucleic acids research, 48, 10413-10427. doi:10.1093/nar/gkaa744. https://pubmed.ncbi.nlm.nih.gov/32960271/
6. Gebhardt, Anna, Habjan, Matthias, Benda, Christian, Habermann, Bianca, Pichlmair, Andreas. 2015. mRNA export through an additional cap-binding complex consisting of NCBP1 and NCBP3. In Nature communications, 6, 8192. doi:10.1038/ncomms9192. https://pubmed.ncbi.nlm.nih.gov/26382858/
7. Li, Jianrong, Zheng, Lin, Song, Liying, Bai, Wenqi, Qi, Liqiang. 2023. Identification and validation of N7 -methylguanosine-associated gene NCBP1 as prognostic and immune-associated biomarkers in breast cancer patients. In Journal of cellular and molecular medicine, 28, e18067. doi:10.1111/jcmm.18067. https://pubmed.ncbi.nlm.nih.gov/38071502/
8. Clarke, Bradley P, Angelos, Alexia E, Mei, Menghan, Xie, Yihu, Ren, Yi. 2024. Cryo-EM structure of the CBC-ALYREF complex. In eLife, 12, . doi:10.7554/eLife.91432. https://pubmed.ncbi.nlm.nih.gov/39282949/
9. Park, Yeonkyoung, Park, Joori, Kim, Yoon Ki. 2017. Crosstalk between translation and the aggresome-autophagy pathway. In Autophagy, 14, 1079-1081. doi:10.1080/15548627.2017.1358849. https://pubmed.ncbi.nlm.nih.gov/28837386/
품질 관리 기준
정자 검사
동결 보존 전: 정자 농도 측정 및 정자 생존율 평가.
동결 보존 후: 각 배치에서 동결 보존된 정자 바이알 1개를 선택하여 체외수정(in vitro fertilization)에 사용합니다.
Environmental Standards:
SPFAvailable Region:
GlobalSource:
Cyagen문의하기
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