Ifi27-KO Mouse
Common Name
Ifi27-KO
제품 ID
S-KO-10347
Backgroud
C57BL/6JCya
품종 계통계통 ID
KOCMP-52668-Ifi27-B6J-VA
상태
이 마우스 계통을 논문에서 사용할 경우, “Ifi27-KO Mouse (카탈로그 번호 S-KO-10347)은 Cyagen에서 구입하였습니다.”라고 명시해 주시기 바랍니다.
구매 가능한 제품 종류
연령
Genotype
성별
수량
표준 제공 조건은 최소 3마리의 이형접합(heterozygous) 보균자를 보장합니다. 동형접합(homozygous) 보균자 및/또는 특정 성별에 대한 브리딩 서비스도 제공됩니다.
기본 정보
품종 계통
Ifi27-KO
품종 계통계통 ID
KOCMP-52668-Ifi27-B6J-VA
유전자명
제품 ID
S-KO-10347
유전자 별칭
ISG12a, Ifi27l1, D12Ertd647e, 1110013J02Rik, 2900026P10Rik
배경
C57BL/6JCya
NCBI ID
변형 내용
Conventional knockout
염색체
Chr 12
Phenotype
Datasheet
적용 분야
--
품종 계통 설명
Ensembl 전사체 ID
ENSMUST00000085065
NCBI 전사체 ID
NM_026790
타겟 영역
Exon 3~7
유효 영역 크기
~4.8 kb
유전자 연구 개요
IFI27, also known as IFN-α-inducible protein 27, is an interferon-stimulated gene (ISG) that plays diverse roles in the immune response, metabolism, and disease [2,3,4,6,8]. It is involved in pathways such as the innate immune response, regulation of the viral life cycle, and JAK-STAT signaling pathway [2]. IFI27 has been associated with various diseases including viral infections, cancer, and inflammatory disorders, highlighting its potential as a biomarker and therapeutic target [2,3,4,5,8,9].
In adipocytes, Ifi27-knockout leads to reduced expression of key enzymes in the tricarboxylic acid cycle, subunits of the electron transport chain, and uncoupling protein 1 (Ucp1), resulting in impaired oxygen consumption and defective thermogenesis [1,7]. This indicates that Ifi27 is crucial for mitochondrial function and adipocyte thermogenic adaptation [1,7]. In the context of viral infections, overexpression of IFI27 positively regulates DENV-2 infection, while knockdown has the opposite effect [2]. In HIV-1 infection, IFI27 is upregulated and can suppress HIV-1 replication by degrading the viral gag protein through the ubiquitin-proteasome pathway [4].
In conclusion, IFI27 is a multifunctional gene. Through gene knockout studies in adipocytes, its importance in mitochondrial function and thermogenesis has been revealed. In viral infections, its role in modulating the infection process has been demonstrated, suggesting that it could be a potential therapeutic target for treating viral diseases like HIV and dengue [1,2,4,7].
References:
1. Cui, Xuan, Liu, Haojie, Shi, Ting, Tang, Qi-Qun, Pan, Dongning. 2023. IFI27 Integrates Succinate and Fatty Acid Oxidation to Promote Adipocyte Thermogenic Adaption. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 10, e2301855. doi:10.1002/advs.202301855. https://pubmed.ncbi.nlm.nih.gov/37544897/
2. Jiang, Cheng, He, Cong, Kan, Jing, Zhou, Tao, Yang, Yi. . Integrative bulk and single-cell transcriptome profiling analysis reveals IFI27 as a novel interferon-stimulated gene in dengue. In Journal of medical virology, 95, e28706. doi:10.1002/jmv.28706. https://pubmed.ncbi.nlm.nih.gov/36971141/
3. Huang, Huijuan, Lv, Jiannan, Huang, Yonglun, Li, Hongmian, Qin, Yaqin. . IFI27 is a potential therapeutic target for HIV infection. In Annals of medicine, 54, 314-325. doi:10.1080/07853890.2021.1995624. https://pubmed.ncbi.nlm.nih.gov/35068272/
4. He, Wen-Qiang, Pang, Wei, Li, Na, Yang, Liu-Meng, Zheng, Yong-Tang. 2024. IFI27 inhibits HIV-1 replication by degrading Gag protein through the ubiquitin-proteasome pathway. In Journal of virology, 98, e0135624. doi:10.1128/jvi.01356-24. https://pubmed.ncbi.nlm.nih.gov/39475279/
5. Shojaei, Maryam, Shamshirian, Amir, Monkman, James, Short, Kirsty R, Tang, Benjamin. 2023. IFI27 transcription is an early predictor for COVID-19 outcomes, a multi-cohort observational study. In Frontiers in immunology, 13, 1060438. doi:10.3389/fimmu.2022.1060438. https://pubmed.ncbi.nlm.nih.gov/36685600/
6. Villamayor, Laura, López-García, Darío, Rivero, Vanessa, Nogales, Aitor, DeDiego, Marta L. 2023. The IFN-stimulated gene IFI27 counteracts innate immune responses after viral infections by interfering with RIG-I signaling. In Frontiers in microbiology, 14, 1176177. doi:10.3389/fmicb.2023.1176177. https://pubmed.ncbi.nlm.nih.gov/37187533/
7. Jin, Weiwei, Jin, Wenfang, Pan, Dongning. 2018. Ifi27 is indispensable for mitochondrial function and browning in adipocytes. In Biochemical and biophysical research communications, 501, 273-279. doi:10.1016/j.bbrc.2018.04.234. https://pubmed.ncbi.nlm.nih.gov/29730295/
8. Shojaei, Maryam, McLean, Anthony S. 2025. Interferon-stimulated gene IFI27 as a multifaceted candidate target in precision medicine. In Trends in immunology, 46, 219-228. doi:10.1016/j.it.2025.01.008. https://pubmed.ncbi.nlm.nih.gov/40044530/
9. Zhang, Ying, Chen, Chuangzhen, Liu, Zhaoyong, Hu, Wang, Lin, Zhixiong. 2022. PABPC1-induced stabilization of IFI27 mRNA promotes angiogenesis and malignant progression in esophageal squamous cell carcinoma through exosomal miRNA-21-5p. In Journal of experimental & clinical cancer research : CR, 41, 111. doi:10.1186/s13046-022-02339-9. https://pubmed.ncbi.nlm.nih.gov/35346324/
품질 관리 기준
정자 검사
동결 보존 전: 정자 농도 측정 및 정자 생존율 평가.
동결 보존 후: 각 배치에서 동결 보존된 정자 바이알 1개를 선택하여 체외수정(in vitro fertilization)에 사용합니다.
Environmental Standards:
SPFAvailable Region:
GlobalSource:
Cyagen문의하기
맞춤형 동물 모델 관련 상담을 위해 Cyagen 전문가와 연락해 보세요. 아래 양식을 작성하여 상담을 시작하거나 견적을 요청하시기 바랍니다.
Cyagen은 고객님의 개인정보를 소중히 여깁니다. 최신 제품, 서비스 및 인사이트를 안내드리고자 합니다. 고객님의 수신 설정은 다음과 같습니다:
해당 커뮤니케이션은 언제든지 수신 거부하실 수 있습니다. 수신 거부 방법 및 데이터 보호에 대한 자세한 내용은 개인정보처리방침을 참고해 주시기 바랍니다.
아래 버튼을 클릭함으로써, 요청하신 콘텐츠 제공을 위해 본 양식을 통해 제출된 개인정보를 Cyagen이 저장 및 처리하는 데 동의하게 됩니다.