Zbp1-KO Mouse
Common Name
Zbp1-KO
제품 ID
S-KO-11088
Backgroud
C57BL/6JCya
품종 계통계통 ID
KOCMP-58203-Zbp1-B6J-VA
상태
이 마우스 계통을 논문에서 사용할 경우, “Zbp1-KO Mouse (카탈로그 번호 S-KO-11088)은 Cyagen에서 구입하였습니다.”라고 명시해 주시기 바랍니다.
구매 가능한 제품 종류
연령
Genotype
성별
수량
표준 제공 조건은 최소 3마리의 이형접합(heterozygous) 보균자를 보장합니다. 동형접합(homozygous) 보균자 및/또는 특정 성별에 대한 브리딩 서비스도 제공됩니다.
기본 정보
품종 계통
Zbp1-KO
품종 계통계통 ID
KOCMP-58203-Zbp1-B6J-VA
유전자명
제품 ID
S-KO-11088
유전자 별칭
Dai, Dlm1, mZaDLM, 2010010H03Rik
배경
C57BL/6JCya
유전자 공식 전체 명칭
Z-DNA binding protein 1
NCBI ID
변형 내용
Conventional knockout
염색체
Chr 2
Phenotype
Datasheet
적용 분야
--
품종 계통 설명
Ensembl 전사체 ID
ENSMUST00000029018
NCBI 전사체 ID
NM_021394
타겟 영역
Exon 2~7
유효 영역 크기
~9.9 kb
유전자 연구 개요
Zbp1, also known as Z-DNA binding protein 1, DAI or DLM-1, is a crucial innate immune sensor that binds to Z-form nucleic acids (Z-DNA and Z-RNA) via its Zα domains and contains receptor-interacting protein homotypic interaction motif (RHIM) domains for protein-protein interactions [4,7]. It plays a vital role in regulating cell death, inflammasome activation, and pro-inflammatory responses, which are associated with pathways like pyroptosis, apoptosis, necroptosis (collectively known as PANoptosis), type I interferon signaling, and the unfolded protein response. These functions are of great biological importance in host defense against viral infections, maintaining homeostasis, and tumorigenesis [1,3,4,6,8]. Genetic models, such as gene knockout (KO) and conditional knockout (CKO) mouse models, are valuable for studying Zbp1.
In Ripk1mR/mR mice expressing RIPK1 with mutated RHIM, Zα-dependent sensing of endogenous ligands by Zbp1 leads to perinatal lethality, indicating its role in regulating necroptosis and inflammation under specific genetic conditions [7]. Adar1fl/flLysMcre mice lacking ADAR1, which interacts with Zbp1, are resistant to colorectal cancer and melanoma development, but deletion of the ZBP1 Zα2 domain restores tumorigenesis, suggesting Zbp1's role in tumorigenesis regulation [3]. In addition, MLKL-deficient mice show reduced nuclear disruption of lung epithelia, decreased neutrophil recruitment, and increased survival after a lethal dose of influenza A virus, as Zbp1-mediated necroptosis is affected, highlighting Zbp1's role in influenza-related pathogenesis [2]. Mice lacking Zbp1 or IFN-I signaling are protected from Doxorubicin-induced cardiotoxicity, revealing Zbp1's contribution to this form of cardiotoxicity through its role in mitochondrial DNA sensing and IFN-I signaling [5].
In conclusion, Zbp1 is a key regulator in multiple biological processes. Through KO/CKO mouse models, its functions in viral infection-related cell death, tumorigenesis, and cardiotoxicity have been well-demonstrated. These model-based studies provide insights into Zbp1's essential biological functions, which may offer potential therapeutic targets for infectious, inflammatory, and cancer diseases.
References:
1. Zheng, Min, Kanneganti, Thirumala-Devi. 2020. The regulation of the ZBP1-NLRP3 inflammasome and its implications in pyroptosis, apoptosis, and necroptosis (PANoptosis). In Immunological reviews, 297, 26-38. doi:10.1111/imr.12909. https://pubmed.ncbi.nlm.nih.gov/32729116/
2. Zhang, Ting, Yin, Chaoran, Boyd, David F, Green, Douglas R, Balachandran, Siddharth. . Influenza Virus Z-RNAs Induce ZBP1-Mediated Necroptosis. In Cell, 180, 1115-1129.e13. doi:10.1016/j.cell.2020.02.050. https://pubmed.ncbi.nlm.nih.gov/32200799/
3. Karki, Rajendra, Sundaram, Balamurugan, Sharma, Bhesh Raj, Vogel, Peter, Kanneganti, Thirumala-Devi. . ADAR1 restricts ZBP1-mediated immune response and PANoptosis to promote tumorigenesis. In Cell reports, 37, 109858. doi:10.1016/j.celrep.2021.109858. https://pubmed.ncbi.nlm.nih.gov/34686350/
4. Kuriakose, Teneema, Kanneganti, Thirumala-Devi. 2017. ZBP1: Innate Sensor Regulating Cell Death and Inflammation. In Trends in immunology, 39, 123-134. doi:10.1016/j.it.2017.11.002. https://pubmed.ncbi.nlm.nih.gov/29236673/
5. Lei, Yuanjiu, VanPortfliet, Jordyn J, Chen, Yi-Fan, Li, Pingwei, West, A Phillip. 2023. Cooperative sensing of mitochondrial DNA by ZBP1 and cGAS promotes cardiotoxicity. In Cell, 186, 3013-3032.e22. doi:10.1016/j.cell.2023.05.039. https://pubmed.ncbi.nlm.nih.gov/37352855/
6. Karki, Rajendra, Kanneganti, Thirumala-Devi. 2023. ADAR1 and ZBP1 in innate immunity, cell death, and disease. In Trends in immunology, 44, 201-216. doi:10.1016/j.it.2023.01.001. https://pubmed.ncbi.nlm.nih.gov/36710220/
7. Jiao, Huipeng, Wachsmuth, Laurens, Kumari, Snehlata, Kaiser, William J, Pasparakis, Manolis. 2020. Z-nucleic-acid sensing triggers ZBP1-dependent necroptosis and inflammation. In Nature, 580, 391-395. doi:10.1038/s41586-020-2129-8. https://pubmed.ncbi.nlm.nih.gov/32296175/
8. Gomes, Marco Túlio R, Guimarães, Erika S, Oliveira, Sergio C. 2025. ZBP1 senses Brucella abortus DNA triggering type I interferon signaling pathway and unfolded protein response activation. In Frontiers in immunology, 15, 1511949. doi:10.3389/fimmu.2024.1511949. https://pubmed.ncbi.nlm.nih.gov/39850894/
품질 관리 기준
정자 검사
동결 보존 전: 정자 농도 측정 및 정자 생존율 평가.
동결 보존 후: 각 배치에서 동결 보존된 정자 바이알 1개를 선택하여 체외수정(in vitro fertilization)에 사용합니다.
Environmental Standards:
SPFAvailable Region:
GlobalSource:
Cyagen출판물
ImmunoHorizons
2026-04-16
ZBP1 mediated restriction of Toxoplasma gondii in both the early acute and chronic brain stages of infection
자세히 보기
The Journal of Clinical Investigation
2024-05-14
ZBP1-mediated apoptosis and inflammation exacerbate steatotic liver ischemia/reperfusion injury
자세히 보기
Cellular & Molecular Immunology
2023-11-26
Integrated NLRP3, AIM2, NLRC4, Pyrin inflammasome activation and assembly drive PANoptosis
자세히 보기
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