Rnf115-KO Mouse
Common Name
Rnf115-KO
제품 ID
S-KO-12474
Backgroud
C57BL/6JCya
품종 계통계통 ID
KOCMP-67845-Rnf115-B6J-VA
상태
이 마우스 계통을 논문에서 사용할 경우, “Rnf115-KO Mouse (카탈로그 번호 S-KO-12474)은 Cyagen에서 구입하였습니다.”라고 명시해 주시기 바랍니다.
구매 가능한 제품 종류
연령
Genotype
성별
수량
표준 제공 조건은 최소 3마리의 이형접합(heterozygous) 보균자를 보장합니다. 동형접합(homozygous) 보균자 및/또는 특정 성별에 대한 브리딩 서비스도 제공됩니다.
기본 정보
품종 계통
Rnf115-KO
품종 계통계통 ID
KOCMP-67845-Rnf115-B6J-VA
유전자명
제품 ID
S-KO-12474
유전자 별칭
Zfp364, 2610028E05Rik
배경
C57BL/6JCya
NCBI ID
변형 내용
Conventional knockout
염색체
Chr 3
Phenotype
Datasheet
적용 분야
--
품종 계통 설명
Ensembl 전사체 ID
ENSMUST00000029740
NCBI 전사체 ID
NM_026406.3
타겟 영역
Exon 4
유효 영역 크기
~1.7 kb
유전자 연구 개요
Rnf115, also known as breast cancer-associated gene 2 (BCA2), is an E3 ubiquitin ligase. It is involved in multiple biological processes, regulating immune responses, autophagy, and cancer-related pathways [1,2,3,4,5,6,7,8,9,10]. Ubiquitination, a key function of Rnf115, impacts the stability and function of target proteins, influencing various cellular activities.
In gene knockout studies, Rnf115 deletion protected mice from acute liver injury by promoting autophagy and inhibiting the inflammatory response [1]. Rnf115 -/- livers showed increased autophagy, removing damaged mitochondria and reducing hepatocyte apoptosis. Additionally, Rnf115 inactivation inhibited M1-type macrophage activation. In Trex1 -/- mice and STINGN153S/WT bone marrow chimeric mice, knockout of Rnf115 alleviated STING-mediated inflammation and autoimmunity [2]. In Rnf115 -/- mice, there was also altered sensitivity to viral infections; they were hypo-sensitive to EMCV and hyper-sensitive to HSV-1 due to Rnf115's dual-role in ubiquitinating MAVS and MITA [4]. Rnf115 -/- mice also showed enhanced phagosomal maturation and reduced tissue damage in response to S. aureus infection [9]. In contrast, in cancer-related studies, overexpression of Rnf115 in thyroid carcinoma, lung adenocarcinoma, and gastric cancer promoted tumor progression [5,7,6].
In conclusion, Rnf115 is a crucial E3 ubiquitin ligase involved in immune regulation, autophagy, and cancer development. Gene knockout mouse models have significantly contributed to understanding its role in acute liver injury, autoimmune diseases, viral infections, bacterial infections, and cancer. These findings provide potential therapeutic targets for related diseases.
References:
1. Feng, Jinqiu, Ye, Shufang, Hai, Bao, Lu, Wenping, Chen, Yingyu. 2023. RNF115/BCA2 deficiency alleviated acute liver injury in mice by promoting autophagy and inhibiting inflammatory response. In Cell death & disease, 14, 855. doi:10.1038/s41419-023-06379-7. https://pubmed.ncbi.nlm.nih.gov/38129372/
2. Zhang, Zhi-Dong, Shi, Chang-Rui, Li, Fang-Xu, Zhong, Bo, Lin, Dandan. 2024. Disulfiram ameliorates STING/MITA-dependent inflammation and autoimmunity by targeting RNF115. In Cellular & molecular immunology, 21, 275-291. doi:10.1038/s41423-024-01131-3. https://pubmed.ncbi.nlm.nih.gov/38267694/
3. Zhang, Zhi-Dong, Li, Hong-Xu, Gan, Hu, Zhong, Bo, Lin, Dandan. 2022. RNF115 Inhibits the Post-ER Trafficking of TLRs and TLRs-Mediated Immune Responses by Catalyzing K11-Linked Ubiquitination of RAB1A and RAB13. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 9, e2105391. doi:10.1002/advs.202105391. https://pubmed.ncbi.nlm.nih.gov/35343654/
4. Zhang, Zhi-Dong, Xiong, Tian-Chen, Yao, Shu-Qi, Lin, Dandan, Zhong, Bo. 2020. RNF115 plays dual roles in innate antiviral responses by catalyzing distinct ubiquitination of MAVS and MITA. In Nature communications, 11, 5536. doi:10.1038/s41467-020-19318-3. https://pubmed.ncbi.nlm.nih.gov/33139700/
5. Zhu, Jinxiang, Guo, Longwei, Dai, Hao, Zhao, Qian, Xu, Chongwen. 2024. RNF115 aggravates tumor progression through regulation of CDK10 degradation in thyroid carcinoma. In Cell biology and toxicology, 40, 14. doi:10.1007/s10565-024-09845-w. https://pubmed.ncbi.nlm.nih.gov/38376606/
6. Li, Riyong, Gu, Zhaohui, Zhang, Xuan, Lv, Ping, Chen, Yingyu. 2020. RNF115 deletion inhibits autophagosome maturation and growth of gastric cancer. In Cell death & disease, 11, 810. doi:10.1038/s41419-020-03011-w. https://pubmed.ncbi.nlm.nih.gov/32980859/
7. Wu, Xiao-Ting, Wang, Yu-Han, Cai, Xiao-Yue, Lu, Wen-Feng, Zhang, Ming. 2021. RNF115 promotes lung adenocarcinoma through Wnt/β-catenin pathway activation by mediating APC ubiquitination. In Cancer & metabolism, 9, 7. doi:10.1186/s40170-021-00243-y. https://pubmed.ncbi.nlm.nih.gov/33509267/
8. Luo, Zhigang, Ye, Xin, Shou, Feng, Li, Fugang, Wang, Gang. 2020. RNF115-mediated ubiquitination of p53 regulates lung adenocarcinoma proliferation. In Biochemical and biophysical research communications, 530, 425-431. doi:10.1016/j.bbrc.2020.05.061. https://pubmed.ncbi.nlm.nih.gov/32553631/
9. Bilkei-Gorzo, Orsolya, Heunis, Tiaan, Marín-Rubio, José Luis, Härtlova, Anetta, Trost, Matthias. 2022. The E3 ubiquitin ligase RNF115 regulates phagosome maturation and host response to bacterial infection. In The EMBO journal, 41, e108970. doi:10.15252/embj.2021108970. https://pubmed.ncbi.nlm.nih.gov/36281581/
10. Gu, Zhaohui, Feng, Jinqiu, Ye, Shufang, Zhu, Bin, Chen, Yingyu. 2025. RNF115 deficiency upregulates autophagy and inhibits hepatocellular carcinoma growth. In Chinese medical journal, 138, 754-756. doi:10.1097/CM9.0000000000003466. https://pubmed.ncbi.nlm.nih.gov/39932173/
품질 관리 기준
정자 검사
동결 보존 전: 정자 농도 측정 및 정자 생존율 평가.
동결 보존 후: 각 배치에서 동결 보존된 정자 바이알 1개를 선택하여 체외수정(in vitro fertilization)에 사용합니다.
Environmental Standards:
SPFAvailable Region:
GlobalSource:
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