Ccdc7b-KO Mouse

Ccdc7b, also known as Biot2-L, contains a conserved domain-Ccdc7. It is highly expressed in adult testis, suggesting its involvement in testis development and spermatogonia [3].

RNA interference against Biot2 (a related gene, potentially an alias) inhibits the growth of tumor cells in vitro and in vivo. Treating B16 murine melanoma, LL/2 murine Lewis lung carcinoma and CT26 murine colorectal adenocarcinoma with special shRNA targeting Biot2 significantly reduces their proliferation rate in vitro [1]. Silencing Biot2 in CT26 cells by RNA interference also induces G1 phase arrest and apoptosis through regulation of p16 and p21 [2].

In conclusion, Ccdc7b (Biot2-L) seems to play a role in testis-related biological processes. The related gene Biot2 shows potential as a target for biotherapy and gene therapy in cancer, as demonstrated by RNA interference studies, which help in understanding its function in tumorigenesis.

References:

1. Wang, Chun-Ting, Zhang, Peng, Wang, Yong-Sheng, Yang, Han-Shuo, Wei, Yu-Quan. 2009. RNA interference against Biot2, a novel mouse testis-specific gene, inhibits the growth of tumor cells. In Cellular & molecular biology letters, 14, 363-76. doi:10.2478/s11658-009-0004-6. https://pubmed.ncbi.nlm.nih.gov/19277478/

2. Zhou, Cong, Zhang, Peng, Xu, Guang-Chao, Zeng, Qi, Wang, Chun-Ting. . RNA interference of Biot2 induces G1 phase arrest and apoptosis in mouse colorectal cancer cell line. In Oncology research, 22, 93-103. doi:10.3727/096504014X14146137738583. https://pubmed.ncbi.nlm.nih.gov/25706396/

3. Wang, Hao, Zhang, Peng, Wang, Chun-Ting. . [Analysis of expression pattern of a novel testis-highly expressed gene Biot2-L and the primary study on its role in testis development]. In Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition, 40, 853-6. doi:. https://pubmed.ncbi.nlm.nih.gov/19950598/