Pdpn-KO Mouse
Common Name
Pdpn-KO
제품 ID
S-KO-16934
Backgroud
C57BL/6JCya
품종 계통계통 ID
KOCMP-14726-Pdpn-B6J-VA
상태
이 마우스 계통을 논문에서 사용할 경우, “Pdpn-KO Mouse (카탈로그 번호 S-KO-16934)은 Cyagen에서 구입하였습니다.”라고 명시해 주시기 바랍니다.
구매 가능한 제품 종류
연령
Genotype
성별
수량
표준 제공 조건은 최소 3마리의 이형접합(heterozygous) 보균자를 보장합니다. 동형접합(homozygous) 보균자 및/또는 특정 성별에 대한 브리딩 서비스도 제공됩니다.
기본 정보
품종 계통
Pdpn-KO
품종 계통계통 ID
KOCMP-14726-Pdpn-B6J-VA
유전자명
제품 ID
S-KO-16934
유전자 별칭
E11, T1a, Gp38, OTS-8, T1alpha, RANDAM-2, T1-alpha
배경
C57BL/6JCya
NCBI ID
변형 내용
Conventional knockout
염색체
Chr 4
Phenotype
Datasheet
적용 분야
--
품종 계통 설명
Ensembl 전사체 ID
ENSMUST00000030317
NCBI 전사체 ID
NM_010329.3
타겟 영역
Exon 2
유효 영역 크기
~0.8 kb
유전자 연구 개요
Podoplanin (PDPN), a transmembrane receptor also known as E11 antigen and gp38, is involved in multiple physiological and pathological processes. It plays roles in cell motility, invasion, tumor metastasis, blood vessel formation, and platelet activation. PDPN interacts with C-type lectin-like receptor-2 (CLEC-2) in relevant pathways [3,7,8].
In cancer research, knockdown of PDPN radiosensitized glioma cell lines and glioma-stem-like cells (GSCs), improving overall survival in an intracranial xenograft mouse model, indicating its role in radiation resistance and tumor aggressiveness in gliomas [2]. In oral squamous cell carcinoma (OSCC), PDPN + cancer-associated fibroblasts (CAFs) promoted OSCC progression by transferring exosomal lncRNA FTX to inhibit ferroptosis in OSCC cells [1]. In gastric cancer, PDPN + CAFs enhanced angiogenesis via AKT/NF-κB activation and the CCL2-ACKR1 axis [4]. In breast cancer, PDPN-expressing macrophages promoted lymphangiogenesis and lymphoinvasion [5]. In thyroid carcinoma cells, PDPN affected invasiveness by inducing ezrin, radixin and moesin (E/R/M) phosphorylation in association with matrix metalloproteinases [6]. In glioblastoma multiforme (GBM), PDPN promoted tumor metastasis [7]. In canine malignant melanoma, silencing of Pdpn induced apoptosis and cell cycle arrest [9].
In summary, PDPN is crucial in processes like cell proliferation, tumor-related angiogenesis, metastasis, and radiation resistance. The use of gene-knockdown models, similar to gene knockout in concept, has revealed its role in various cancer-related disease conditions, providing potential therapeutic targets for these cancers.
References:
1. Li, Yaoyin, Ma, Zeyi, Li, Weiyu, Cheng, Bin, Xia, Juan. 2023. PDPN+ CAFs facilitate the motility of OSCC cells by inhibiting ferroptosis via transferring exosomal lncRNA FTX. In Cell death & disease, 14, 759. doi:10.1038/s41419-023-06280-3. https://pubmed.ncbi.nlm.nih.gov/37993428/
2. Modrek, Aram S, Eskilsson, Eskil, Ezhilarasan, Ravesanker, Aldape, Kenneth D, Sulman, Erik P. 2022. PDPN marks a subset of aggressive and radiation-resistant glioblastoma cells. In Frontiers in oncology, 12, 941657. doi:10.3389/fonc.2022.941657. https://pubmed.ncbi.nlm.nih.gov/36059614/
3. Gao, Yi, Qin, Lihong, Yang, Yuwei, Zhang, Guoliang, Zhao, Zhihui. 2017. PDPN gene promotes the proliferation of immature Bovine Sertoli cells in vitro. In Animal reproduction science, 179, 35-43. doi:10.1016/j.anireprosci.2017.01.014. https://pubmed.ncbi.nlm.nih.gov/28233584/
4. Zhao, Zhenxiong, Sun, Hui, Liu, Yingxue, Sheng, Weiqi, Xu, Mi-Die. 2025. PDPN+ cancer-associated fibroblasts enhance gastric cancer angiogenesis via AKT/NF-κB activation and the CCL2-ACKR1 axis. In MedComm, 6, e70037. doi:10.1002/mco2.70037. https://pubmed.ncbi.nlm.nih.gov/39764562/
5. Bieniasz-Krzywiec, Paweł, Martín-Pérez, Rosa, Ehling, Manuel, Van Ginderachter, Jo A, Mazzone, Massimiliano. 2019. Podoplanin-Expressing Macrophages Promote Lymphangiogenesis and Lymphoinvasion in Breast Cancer. In Cell metabolism, 30, 917-936.e10. doi:10.1016/j.cmet.2019.07.015. https://pubmed.ncbi.nlm.nih.gov/31447322/
6. Sikorska, Justyna, Gaweł, Damian, Domek, Hanna, Rudzińska, Magdalena, Czarnocka, Barbara. 2019. Podoplanin (PDPN) affects the invasiveness of thyroid carcinoma cells by inducing ezrin, radixin and moesin (E/R/M) phosphorylation in association with matrix metalloproteinases. In BMC cancer, 19, 85. doi:10.1186/s12885-018-5239-z. https://pubmed.ncbi.nlm.nih.gov/30654768/
7. Sharma, Bharti, Agriantonis, George, Shafaee, Zahra, Ghanta, Hima, Whittington, Jennifer. 2024. Role of Podoplanin (PDPN) in Advancing the Progression and Metastasis of Glioblastoma Multiforme (GBM). In Cancers, 16, . doi:10.3390/cancers16234051. https://pubmed.ncbi.nlm.nih.gov/39682237/
8. Nara, Rikuto, Notoh, Hinako, Sasaki, Tomoyuki, Matsushita, Tadashi, Tamura, Shogo. 2024. PDPN/CLEC-2 axis modulates megakaryocyte subtypes in a hematopoietic stem cell-regulating megakaryocyte-dominant manner. In Thrombosis research, 245, 109230. doi:10.1016/j.thromres.2024.109230. https://pubmed.ncbi.nlm.nih.gov/39615442/
9. Shinada, Masahiro, Kato, Daiki, Kamoto, Satoshi, Kato, Yukinari, Nakagawa, Takayuki. 2020. PDPN Is Expressed in Various Types of Canine Tumors and Its Silencing Induces Apoptosis and Cell Cycle Arrest in Canine Malignant Melanoma. In Cells, 9, . doi:10.3390/cells9051136. https://pubmed.ncbi.nlm.nih.gov/32380790/
품질 관리 기준
정자 검사
동결 보존 전: 정자 농도 측정 및 정자 생존율 평가.
동결 보존 후: 각 배치에서 동결 보존된 정자 바이알 1개를 선택하여 체외수정(in vitro fertilization)에 사용합니다.
Environmental Standards:
SPFAvailable Region:
GlobalSource:
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