Hif1an-KO Mouse
Common Name
Hif1an-KO
제품 ID
S-KO-17905
Backgroud
C57BL/6JCya
품종 계통계통 ID
KOCMP-319594-Hif1an-B6J-VA
상태
이 마우스 계통을 논문에서 사용할 경우, “Hif1an-KO Mouse (카탈로그 번호 S-KO-17905)은 Cyagen에서 구입하였습니다.”라고 명시해 주시기 바랍니다.
구매 가능한 제품 종류
연령
Genotype
성별
수량
표준 제공 조건은 최소 3마리의 이형접합(heterozygous) 보균자를 보장합니다. 동형접합(homozygous) 보균자 및/또는 특정 성별에 대한 브리딩 서비스도 제공됩니다.
기본 정보
품종 계통
Hif1an-KO
품종 계통계통 ID
KOCMP-319594-Hif1an-B6J-VA
유전자명
제품 ID
S-KO-17905
유전자 별칭
FIH, FIH1, 2310046M24Rik, A830014H24Rik
배경
C57BL/6JCya
NCBI ID
변형 내용
Conventional knockout
염색체
Chr 19
Phenotype
Datasheet
적용 분야
--
품종 계통 설명
Ensembl 전사체 ID
ENSMUST00000040455
NCBI 전사체 ID
NM_176958
타겟 영역
Exon 3
유효 영역 크기
~1.6 kb
유전자 연구 개요
Hif1an, also known as hypoxia-inducible factor 1-alpha subunit suppressor, plays a crucial role in regulating hypoxia-inducible factor 1-alpha (HIF-1α) stability and transcriptional action. It is part of the oxygen-sensing pathway, with the PHD family of oxygen-dependent prolyl hydroxylases and FIH1-mediated asparagine hydroxylation also involved in tuning HIF transcriptional activity [2]. Hif1an is important in various biological processes, and its inappropriate expression is associated with cancer development and immune control [1].
In breast cancer, low Hif1an expression accompanied by less immune infiltration is associated with poor prognosis. High Hif1an expression is linked to good overall survival, and its expression is closely related to chemokines and immune cell infiltration [1].
In keloid formation, circSLC8A1 regulates the miR-181a-5p/HIF1AN axis to restrain the biological functions of human keloid fibroblasts [3].
In cardiomyocytes, pinoresinol diglucoside ameliorates hypoxia/reperfusion-induced injury by regulating miR-142-3p and HIF1AN [4].
In choriocarcinoma, METTL3 promotes miR-21-5p maturation to accelerate choriocarcinoma progression via the HIF1AN-induced inactivation of the HIF1A/VEGF pathway [5].
In hypertrophic scar formation, Rynchopeterine inhibits the formation of hypertrophic scars by regulating the miR-21/HIF1AN axis [6].
In skin flap transplantation, M2 macrophage-derived exosomes induce angiogenesis and increase skin flap survival through HIF1AN/HIF-1α/VEGFA control [7].
In osteoblast differentiation, miR-135-5p promotes osteoblast differentiation by targeting HIF1AN in MC3T3-E1 cells [8].
In conclusion, Hif1an is essential for regulating HIF-1α, which impacts multiple biological processes. Its dysregulation is involved in various diseases such as cancer, keloid, and hypertrophic scar formation, as well as in processes like angiogenesis and osteoblast differentiation. Studies on Hif1an contribute to understanding the underlying mechanisms of these diseases and biological processes, potentially providing new strategies for treatment and prevention.
References：
1. Tang, Shasha, Liu, Dongyang, Fang, Yuan, Wang, Hui, Cai, Fengfeng. 2023. Low expression of HIF1AN accompanied by less immune infiltration is associated with poor prognosis in breast cancer. In Frontiers in oncology, 13, 1080910. doi:10.3389/fonc.2023.1080910. https://pubmed.ncbi.nlm.nih.gov/36816977/
2. Kaelin, William G, Ratcliffe, Peter J. . Oxygen sensing by metazoans: the central role of the HIF hydroxylase pathway. In Molecular cell, 30, 393-402. doi:10.1016/j.molcel.2008.04.009. https://pubmed.ncbi.nlm.nih.gov/18498744/
3. Yuan, Xiaoye, Chen, Baiye, Wang, Xueming. 2022. CircSLC8A1 targets miR-181a-5p/HIF1AN pathway to inhibit the growth, migration and extracellular matrix deposition of human keloid fibroblasts. In Burns : journal of the International Society for Burn Injuries, 49, 622-632. doi:10.1016/j.burns.2022.04.009. https://pubmed.ncbi.nlm.nih.gov/35610079/
4. Wei, Yuan, Xiao, Liang, Yingying, Liu, Haichen, Wang. 2022. Pinoresinol diglucoside ameliorates H/R-induced injury of cardiomyocytes by regulating miR-142-3p and HIF1AN. In Journal of biochemical and molecular toxicology, 36, e23175. doi:10.1002/jbt.23175. https://pubmed.ncbi.nlm.nih.gov/35962614/
5. Ye, Kefan, Li, Lingchuan, Wu, Bao, Wang, Dongjie. 2022. METTL3 m6A-dependently promotes miR-21-5p maturation to accelerate choriocarcinoma progression via the HIF1AN-induced inactivation of the HIF1A/VEGF pathway. In Genes & genomics, 44, 1311-1322. doi:10.1007/s13258-022-01309-x. https://pubmed.ncbi.nlm.nih.gov/36074324/
6. Zhao, Wenbin, Ye, Jianzhou, Yang, Xuesong, Zhang, Qiongyu, Li, Jiaqi. 2024. Rynchopeterine inhibits the formation of hypertrophic scars by regulating the miR-21/HIF1AN axis. In Experimental cell research, 440, 114114. doi:10.1016/j.yexcr.2024.114114. https://pubmed.ncbi.nlm.nih.gov/38823472/
7. Luo, Gaojie, Zhou, Zekun, Cao, Zheming, Tang, Juyu, Qing, Liming. 2023. M2 macrophage-derived exosomes induce angiogenesis and increase skin flap survival through HIF1AN/HIF-1α/VEGFA control. In Archives of biochemistry and biophysics, 751, 109822. doi:10.1016/j.abb.2023.109822. https://pubmed.ncbi.nlm.nih.gov/38030054/
8. Yin, Nuo, Zhu, Longzhang, Ding, Liang, Xue, Feng, Xiao, Haijun. 2019. MiR-135-5p promotes osteoblast differentiation by targeting HIF1AN in MC3T3-E1 cells. In Cellular & molecular biology letters, 24, 51. doi:10.1186/s11658-019-0177-6. https://pubmed.ncbi.nlm.nih.gov/31410089/
품질 관리 기준
정자 검사
동결 보존 전: 정자 농도 측정 및 정자 생존율 평가.
동결 보존 후: 각 배치에서 동결 보존된 정자 바이알 1개를 선택하여 체외수정(in vitro fertilization)에 사용합니다.
Environmental Standards:
SPFAvailable Region:
GlobalSource:
Cyagen문의하기
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