Dgkg-KO Mouse
Common Name
Dgkg-KO
제품 ID
S-KO-18550
Backgroud
C57BL/6JCya
품종 계통계통 ID
KOCMP-110197-Dgkg-B6J-VA
상태
이 마우스 계통을 논문에서 사용할 경우, “Dgkg-KO Mouse (카탈로그 번호 S-KO-18550)은 Cyagen에서 구입하였습니다.”라고 명시해 주시기 바랍니다.
구매 가능한 제품 종류
연령
Genotype
성별
수량
표준 제공 조건은 최소 3마리의 이형접합(heterozygous) 보균자를 보장합니다. 동형접합(homozygous) 보균자 및/또는 특정 성별에 대한 브리딩 서비스도 제공됩니다.
기본 정보
품종 계통
Dgkg-KO
품종 계통계통 ID
KOCMP-110197-Dgkg-B6J-VA
유전자명
제품 ID
S-KO-18550
유전자 별칭
90kDa, Dagk3, mKIAA4131, 2900055E17Rik, E430001K23Rik
배경
C57BL/6JCya
NCBI ID
변형 내용
Conventional knockout
염색체
Chr 16
Phenotype
Datasheet
적용 분야
--
품종 계통 설명
Ensembl 전사체 ID
ENSMUST00000089925
NCBI 전사체 ID
NM_138650
타겟 영역
Exon 4~5
유효 영역 크기
~1.5 kb
유전자 연구 개요
Dgkg, encoding diacylglycerol kinase gamma, is a key enzyme in glycerophospholipid metabolism and phosphatidylinositol signaling pathways [7]. Diacylglycerol kinases are important regulators of cell signaling, and Dgkg may be involved in various biological processes [4].
In hepatocellular carcinoma (HCC), endothelial Dgkg promoted tumor angiogenesis and immunosuppressive regulatory T-cell differentiation. Hypoxia-induced HIF-1α activates Dgkg transcription, and upregulated Dgkg promotes HCC progression via the ZEB2/TGF-β1 axis. Targeting endothelial Dgkg enhanced the efficiency of dual blockade of PD-1 and VEGFR-2 in a mouse HCC model [1].
In Wagyu cattle, a homozygous missense variant in Dgkg was associated with hepatic fibrinogen storage disease [2].
In an epileptic patient, a heterozygous mutation in Dgkg was found, and an induced pluripotent stem cell line was generated for studying epilepsy mechanisms [3].
In colorectal cancer, Dgkg was hypermethylated, suppressing its expression. Ectopic expression of wild-type and mutant Dgkg suppressed cell migration, invasion, and Rac1 activity, suggesting a tumor-suppressor role [4].
In acute myeloid leukemia, high Dgkg expression was associated with a more favorable prognosis according to the TGCA database [5].
In cortical GABAergic interneurons, Dgkg was dominantly expressed in somatostatin-expressing neurons, and its functional study indicated an alteration in neurite outgrowth [6].
In glioblastoma, a hypoxia-induced alternative splicing transcript Dgkg-Δ exon13 promoted cellular proliferation, migration, and invasion [8].
In conclusion, Dgkg plays diverse roles in multiple biological processes and diseases. Its functions in tumor angiogenesis, immune evasion, and cell migration/invasion in cancers, as well as in neurological and metabolic-related diseases, have been revealed through various research models, including in vivo studies in mouse models. Understanding Dgkg provides insights into disease mechanisms and potential therapeutic targets.
References：
1. Zhang, Liren, Xu, Jiali, Zhou, Suiqing, Li, Qing, Wang, Xuehao. 2023. Endothelial DGKG promotes tumor angiogenesis and immune evasion in hepatocellular carcinoma. In Journal of hepatology, 80, 82-98. doi:10.1016/j.jhep.2023.10.006. https://pubmed.ncbi.nlm.nih.gov/37838036/
2. Jacinto, Joana G P, Wohlsein, Peter, Häfliger, Irene M, Grünberg, Walter, Drögemüller, Cord. 2023. A missense variant in DGKG as a recessive functional variant for hepatic fibrinogen storage disease in Wagyu cattle. In Journal of veterinary internal medicine, 37, 2631-2637. doi:10.1111/jvim.16865. https://pubmed.ncbi.nlm.nih.gov/37681469/
3. Liu, Ya-Qing, Ling, Tiao-Wen, Wang, Hong-Yan, Song, Wen-Jun, Wang, Tian-Cheng. 2022. Generation of an integration-free induced pluripotent stem cell line (LZUSHI001-A) from an epileptic patient with DGKG mutation. In Stem cell research, 61, 102768. doi:10.1016/j.scr.2022.102768. https://pubmed.ncbi.nlm.nih.gov/35421845/
4. Kai, Masahiro, Yamamoto, Eiichiro, Sato, Akiko, Toyota, Minoru, Suzuki, Hiromu. 2017. Epigenetic silencing of diacylglycerol kinase gamma in colorectal cancer. In Molecular carcinogenesis, 56, 1743-1752. doi:10.1002/mc.22631. https://pubmed.ncbi.nlm.nih.gov/28218473/
5. Gravina, Teresa, Boggio, Chiara Maria Teresa, Gorla, Elisa, Corà, Davide, Baldanzi, Gianluca. 2023. Role of Diacylglycerol Kinases in Acute Myeloid Leukemia. In Biomedicines, 11, . doi:10.3390/biomedicines11071877. https://pubmed.ncbi.nlm.nih.gov/37509516/
6. Fukumoto, Keita, Tamada, Kota, Toya, Tsuyoshi, Yanagawa, Yuchio, Takumi, Toru. 2017. Identification of genes regulating GABAergic interneuron maturation. In Neuroscience research, 134, 18-29. doi:10.1016/j.neures.2017.11.010. https://pubmed.ncbi.nlm.nih.gov/29203264/
7. Taniguchi-Ponciano, Keiko, Hinojosa-Alvarez, Silvia, Hernandez-Perez, Jesus, Marrero-Rodriguez, Daniel, Mercado, Moises. 2024. Longitudinal multiomics analysis of aggressive pituitary neuroendocrine tumors: comparing primary and recurrent tumors from the same patient, reveals genomic stability and heterogeneous transcriptomic profiles with alterations in metabolic pathways. In Acta neuropathologica communications, 12, 142. doi:10.1186/s40478-024-01796-x. https://pubmed.ncbi.nlm.nih.gov/39217365/
8. Yang, Ming, Chu, Liangzhao, Lin, Shukai, Han, Feng, Liu, Jian. 2025. The alternatively spliced diacylglycerol kinase gamma-Δ exon13 transcript generated under hypoxia promotes glioblastoma progression. In Oncology research, 33, 1189-1198. doi:10.32604/or.2024.055102. https://pubmed.ncbi.nlm.nih.gov/40296900/
품질 관리 기준
정자 검사
동결 보존 전: 정자 농도 측정 및 정자 생존율 평가.
동결 보존 후: 각 배치에서 동결 보존된 정자 바이알 1개를 선택하여 체외수정(in vitro fertilization)에 사용합니다.
Environmental Standards:
SPFAvailable Region:
GlobalSource:
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