Pfkm-KO Mouse
Common Name
Pfkm-KO
제품 ID
S-KO-18671
Backgroud
C57BL/6JCya
품종 계통계통 ID
KOCMP-18642-Pfkm-B6J-VA
상태
이 마우스 계통을 논문에서 사용할 경우, “Pfkm-KO Mouse (카탈로그 번호 S-KO-18671)은 Cyagen에서 구입하였습니다.”라고 명시해 주시기 바랍니다.
구매 가능한 제품 종류
연령
Genotype
성별
수량
표준 제공 조건은 최소 3마리의 이형접합(heterozygous) 보균자를 보장합니다. 동형접합(homozygous) 보균자 및/또는 특정 성별에 대한 브리딩 서비스도 제공됩니다.
기본 정보
품종 계통
Pfkm-KO
품종 계통계통 ID
KOCMP-18642-Pfkm-B6J-VA
유전자명
제품 ID
S-KO-18671
유전자 별칭
Pfk4, Pfka, Pfkx, PFK-A, PFK-M, Pfk-4, ATP-PFK
배경
C57BL/6JCya
NCBI ID
변형 내용
Conventional knockout
염색체
Chr 15
Phenotype
Datasheet
적용 분야
--
품종 계통 설명
Ensembl 전사체 ID
ENSMUST00000051226
NCBI 전사체 ID
NM_001163487
타겟 영역
Exon 3~4
유효 영역 크기
~1.8 kb
유전자 연구 개요
PFKM, short for Phosphofructokinase, Muscle, is a key regulatory enzyme in glycolysis. It catalyzes the phosphorylation of fructose-6-phosphate, playing a vital role in energy metabolism pathways. Alterations in PFKM can impact various biological processes and is associated with multiple disease conditions, making it an important gene for functional studies [4,6].
In cancer research, ZEB1 was found to transcriptionally upregulate PFKM, enhancing the Warburg effect and facilitating tumorigenesis and metastasis in hepatocellular carcinoma (HCC). Silencing ZEB1 led to impaired PFKM expression, glycolysis, proliferation, and invasion, which were rescued by exogenous PFKM expression. This indicates the significance of the ZEB1-PFKM axis in HCC carcinogenesis and metastasis [1].
In renal fibrosis, bone marrow mesenchymal stem cell-derived exosomal miR-21a-5p alleviates fibrosis by repressing PFKM expression and attenuating glycolysis in tubular epithelial cells [2].
In osteosarcoma, NAT10-mediated ac4C acetylation drives m6A modification involving YTHDC1-LDHA/PFKM, regulating glycolysis and promoting the disease [3].
In doxorubicin-induced cardiotoxicity, PFKM overexpression inhibits apoptosis, while its silencing promotes apoptosis, with PFKM regulating cell viability and apoptosis through the glycolysis pathway [4].
In sepsis, rTM treatment downregulates PFKM expression in macrophages, protecting mice from sepsis, and HIF-1α regulates PFKM expression through METTL3-mediated m6A modification [5].
In ovarian cancer cells, S-nitrosylation of PFKM at Cys351 by NOS1 stabilizes its tetramer, promoting metabolic reprogramming [7].
In summary, PFKM is crucial for glycolysis and has a far-reaching impact on multiple disease conditions, including cancer, renal fibrosis, osteosarcoma, cardiotoxicity, and sepsis. Gene-knockout or conditional-knockout mouse models (if applicable in these studies) could potentially further clarify its role in these biological processes, providing insights for developing new therapeutic strategies for related diseases.
References：
1. Zhou, Yanming, Lin, Furong, Wan, Tao, Lin, Donghai, Li, Qinxi. 2021. ZEB1 enhances Warburg effect to facilitate tumorigenesis and metastasis of HCC by transcriptionally activating PFKM. In Theranostics, 11, 5926-5938. doi:10.7150/thno.56490. https://pubmed.ncbi.nlm.nih.gov/33897890/
2. Xu, Shihao, Cheuk, Yin Celeste, Jia, Yichen, Shi, Yi, Rong, Ruiming. 2022. Bone marrow mesenchymal stem cell-derived exosomal miR-21a-5p alleviates renal fibrosis by attenuating glycolysis by targeting PFKM. In Cell death & disease, 13, 876. doi:10.1038/s41419-022-05305-7. https://pubmed.ncbi.nlm.nih.gov/36253358/
3. Mei, Zhongting, Shen, Zhihua, Pu, Jiaying, Yuan, Ye, Yang, Lei. 2024. NAT10 mediated ac4C acetylation driven m6A modification via involvement of YTHDC1-LDHA/PFKM regulates glycolysis and promotes osteosarcoma. In Cell communication and signaling : CCS, 22, 51. doi:10.1186/s12964-023-01321-y. https://pubmed.ncbi.nlm.nih.gov/38233839/
4. Zhou, Min, Sun, Xiao, Wang, Chunli, Fang, Chuibi, Hu, Zhenlei. 2022. PFKM inhibits doxorubicin-induced cardiotoxicity by enhancing oxidative phosphorylation and glycolysis. In Scientific reports, 12, 11684. doi:10.1038/s41598-022-15743-0. https://pubmed.ncbi.nlm.nih.gov/35804014/
5. Yao, Chen, Zhu, Hanyong, Ji, Binbin, Pan, Yuchen, Ikezoe, Takayuki. 2024. rTM reprograms macrophages via the HIF-1α/METTL3/PFKM axis to protect mice against sepsis. In Cellular and molecular life sciences : CMLS, 81, 456. doi:10.1007/s00018-024-05489-5. https://pubmed.ncbi.nlm.nih.gov/39549085/
6. Long, Siyu, Zhang, Ran, Yang, Qinni, Zhou, Bin, Zhang, Lin. 2022. Association of PFKM gene polymorphisms and susceptibility to cryptorchidism in a Chinese Han population. In Pediatric surgery international, 38, 1311-1316. doi:10.1007/s00383-022-05167-2. https://pubmed.ncbi.nlm.nih.gov/35838787/
7. Gao, Wenwen, Huang, Mengqiu, Chen, Xi, Hao, Bingtao, Liu, Qiuzhen. 2021. The role of S-nitrosylation of PFKM in regulation of glycolysis in ovarian cancer cells. In Cell death & disease, 12, 408. doi:10.1038/s41419-021-03681-0. https://pubmed.ncbi.nlm.nih.gov/33859186/
품질 관리 기준
정자 검사
동결 보존 전: 정자 농도 측정 및 정자 생존율 평가.
동결 보존 후: 각 배치에서 동결 보존된 정자 바이알 1개를 선택하여 체외수정(in vitro fertilization)에 사용합니다.
Environmental Standards:
SPFAvailable Region:
GlobalSource:
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