Mfge8-KO Mouse
Common Name
Mfge8-KO
제품 ID
S-KO-18834
Backgroud
C57BL/6JCya
품종 계통계통 ID
KOCMP-17304-Mfge8-B6J-VB
상태
이 마우스 계통을 논문에서 사용할 경우, “Mfge8-KO Mouse (카탈로그 번호 S-KO-18834)은 Cyagen에서 구입하였습니다.”라고 명시해 주시기 바랍니다.
구매 가능한 제품 종류
연령
Genotype
성별
수량
표준 제공 조건은 최소 3마리의 이형접합(heterozygous) 보균자를 보장합니다. 동형접합(homozygous) 보균자 및/또는 특정 성별에 대한 브리딩 서비스도 제공됩니다.
기본 정보
품종 계통
Mfge8-KO
품종 계통계통 ID
KOCMP-17304-Mfge8-B6J-VB
유전자명
제품 ID
S-KO-18834
유전자 별칭
P47, MP47, Mfgm, SED1, MFG-E8, lactadherin
배경
C57BL/6JCya
NCBI ID
변형 내용
Conventional knockout
염색체
Chr 7
Phenotype
Datasheet
적용 분야
--
품종 계통 설명
Ensembl 전사체 ID
ENSMUST00000032825
NCBI 전사체 ID
NM_008594
타겟 영역
Exon 2~6
유효 영역 크기
~3.9 kb
유전자 연구 개요
Mfge8, also known as Milk fat globule-epidermal growth factor 8, is a secreted protein that plays diverse roles in various biological processes. It binds to integrin families, such as αVβ3/αVβ5, α8β1, and integrin β3, and activates multiple signaling pathways including PI3K/AKT, ERK/AKT, and Src-FAK-STAT3. It is involved in processes like angiogenesis, fibrosis, efferocytosis, insulin signaling, neurogenesis, cancer progression, and gastric muscle contraction, highlighting its overall biological importance. Genetic models can be valuable in further elucidating its functions [1,2,3,4,5,6,7].
In esophageal squamous cell carcinoma (ESCC), the MFGE8 secreted by cancer-associated fibroblasts (CAFs) promotes tumor angiogenesis, proliferation, drug resistance, and metastasis by activating PI3K/AKT and ERK/AKT pathways via binding to Integrin αVβ3/αVβ5 receptors. Targeting CAFs-secreting MFGE8 or its receptor significantly inhibits ESCC growth and metastasis in vivo [1].
In colorectal cancer, CRC-derived extracellular vesicles containing MFGE8 enhance macrophage efferocytosis of cisplatin-induced apoptotic CRC cells by activating the macrophage αvβ3-Src-FAK-STAT3 signaling pathway. MFGE8 knockout CRC cell lines show that their EVs fail to upregulate macrophage efferocytosis in vitro and in vivo, suggesting MFGE8's role in promoting drug resistance [3].
In rats with experimental subarachnoid hemorrhage (SAH), exogenous recombinant human MFGE8 (rhMFGE8) administration promotes adult hippocampal neurogenesis and improves neurobehavioral recovery by activating the integrin β3/Akt pathway. Silencing integrin β3 or inhibiting the PI3K/Akt pathway abolishes the effects of rhMFGE8 [5].
In conclusion, Mfge8 has a wide-reaching impact on numerous biological processes. Gene knockout models, as seen in ESCC, colorectal cancer, and SAH studies, have revealed its role in cancer progression, drug resistance, and neurogenesis. These findings contribute to understanding the mechanisms of these diseases and suggest potential therapeutic targets related to Mfge8.
References：
1. Liu, Beilei, Zhang, Baifeng, Qi, Jiali, Wang, Xin, Guan, Xin-Yuan. 2023. Targeting MFGE8 secreted by cancer-associated fibroblasts blocks angiogenesis and metastasis in esophageal squamous cell carcinoma. In Proceedings of the National Academy of Sciences of the United States of America, 120, e2307914120. doi:10.1073/pnas.2307914120. https://pubmed.ncbi.nlm.nih.gov/37816055/
2. Lin, Sinan, Wang, Jie, Mukherjee, Pranab K, Fiocchi, Claudio, Rieder, Florian. 2024. Milk fat globule-epidermal growth factor 8 (MFGE8) prevents intestinal fibrosis. In Gut, 73, 1110-1123. doi:10.1136/gutjnl-2022-328608. https://pubmed.ncbi.nlm.nih.gov/38378253/
3. Ma, Zhixin, Sun, Yu, Yu, Yang, Xiang, Xi, Li, Zhigang. 2024. Extracellular vesicles containing MFGE8 from colorectal cancer facilitate macrophage efferocytosis. In Cell communication and signaling : CCS, 22, 295. doi:10.1186/s12964-024-01669-9. https://pubmed.ncbi.nlm.nih.gov/38802814/
4. Datta, Ritwik, Podolsky, Michael J, Yang, Christopher D, Lizama, Carlos O, Atabai, Kamran. 2023. MFGE8 inhibits insulin signaling through PTP1B. In bioRxiv : the preprint server for biology, , . doi:10.1101/2023.05.30.542928. https://pubmed.ncbi.nlm.nih.gov/37398282/
5. Li, Zhen-Yan, Yang, Xian, Wang, Ji-Kai, Liu, Fei, Zuo, Yu-Chun. 2024. MFGE8 promotes adult hippocampal neurogenesis in rats following experimental subarachnoid hemorrhage via modifying the integrin β3/Akt signaling pathway. In Cell death discovery, 10, 359. doi:10.1038/s41420-024-02132-x. https://pubmed.ncbi.nlm.nih.gov/39128910/
6. Ding, Long-Long, Zhang, Meng, Zhang, Tao, Liu, Hui, Liu, Peng-Fei. 2024. MFGE8 promotes gastric cancer progression by activating the IL-6/JAK/STAT3 signaling. In Cellular signalling, 125, 111486. doi:10.1016/j.cellsig.2024.111486. https://pubmed.ncbi.nlm.nih.gov/39490801/
7. Li, Wen, Olseen, Ashley, Xie, Yeming, Wykosky, Jill, Perrino, Brian A. 2021. Mfge8 attenuates human gastric antrum smooth muscle contractions. In Journal of muscle research and cell motility, 42, 219-231. doi:10.1007/s10974-021-09604-y. https://pubmed.ncbi.nlm.nih.gov/34085177/
품질 관리 기준
정자 검사
동결 보존 전: 정자 농도 측정 및 정자 생존율 평가.
동결 보존 후: 각 배치에서 동결 보존된 정자 바이알 1개를 선택하여 체외수정(in vitro fertilization)에 사용합니다.
Environmental Standards:
SPFAvailable Region:
GlobalSource:
Cyagen문의하기
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