Tspan5-KO Mouse
Common Name
Tspan5-KO
제품 ID
S-KO-19010
Backgroud
C57BL/6JCya
품종 계통계통 ID
KOCMP-56224-Tspan5-B6J-VB
상태
이 마우스 계통을 논문에서 사용할 경우, “Tspan5-KO Mouse (카탈로그 번호 S-KO-19010)은 Cyagen에서 구입하였습니다.”라고 명시해 주시기 바랍니다.
구매 가능한 제품 종류
연령
Genotype
성별
수량
표준 제공 조건은 최소 3마리의 이형접합(heterozygous) 보균자를 보장합니다. 동형접합(homozygous) 보균자 및/또는 특정 성별에 대한 브리딩 서비스도 제공됩니다.
기본 정보
품종 계통
Tspan5-KO
품종 계통계통 ID
KOCMP-56224-Tspan5-B6J-VB
유전자명
제품 ID
S-KO-19010
유전자 별칭
NET-4, Tm4sf9, 2810455A09Rik, 4930505M03Rik
배경
C57BL/6JCya
NCBI ID
변형 내용
Conventional knockout
염색체
Chr 3
Phenotype
Datasheet
적용 분야
--
품종 계통 설명
Ensembl 전사체 ID
ENSMUST00000029800
NCBI 전사체 ID
NM_019571
타겟 영역
Exon 4~7
유효 영역 크기
~4.9 kb
유전자 연구 개요
Tspan5, a member of the tetraspanin family, is involved in multiple biological functions. It can interact with specific partner proteins in tetraspanin-enriched microdomains, regulating their subcellular localization and function. It is associated with pathways such as Notch signaling, and plays important roles in processes like cell fusion, epithelial-mesenchymal transition (EMT), and synaptic function, with implications for various diseases [1,2,4].
In placental trophoblastic cells, Tspan5 promotes the EMT process to regulate syncytialization by activating Notch signalling. Knockdown of Tspan5 inhibits cell fusion and EMT-related protein levels [1].
In hepatocellular carcinoma (HCC), Tspan5 promotes EMT and tumour metastasis by activating Notch signalling, and its high expression is associated with poor prognosis. Lentivirus-mediated alteration of Tspan5 expression in HCC cells shows its role in promoting cell migration and tumour metastasis in vitro and in vivo [2].
In alcohol-related disorders, ethanol and acamprosate treatment can down-regulate Tspan5 expression, influencing serotonin and kynurenine concentrations [3].
In the nervous system, Tspan5 knockdown in mouse pyramidal excitatory neurons causes a decrease in spine number due to spine maturation defects, and it promotes neuroligin-1 surface clustering [4]. In rat hippocampal neurons, Tspan5 promotes AMPA receptor exocytosis by interacting with the AP4 complex [5].
In gastric cancer, Tspan5 is down-regulated, and its up-regulation inhibits cell proliferation and tumour growth by modulating cell cycle-related proteins [6].
In HCC, depletion of Tspan5 induces oncogene-induced senescence through the p16INK4a/pRb pathways and affects actin polymerization and MRTF-A-FLNA complex formation [7].
In conclusion, Tspan5 is crucial in multiple biological processes and diseases. Its role in cell fusion, EMT, synaptic function, and tumour-related processes has been revealed through various functional studies including knockdown experiments. These findings contribute to understanding normal development and disease mechanisms, such as in placental development, cancer metastasis, and alcohol-related disorders, providing potential therapeutic targets.
References：
1. Tang, Hai-Yu, Lin, Mei, Liang, Yong-Qian, Yi, Hong-Gan, Yang, Man. 2023. Tspan5 promotes the EMT process to regulate the syncytialization of trophoblast cells by activating Notch signalling. In Zygote (Cambridge, England), 31, 498-506. doi:10.1017/S0967199423000369. https://pubmed.ncbi.nlm.nih.gov/37485669/
2. Xie, Qian, Guo, Huiling, He, Peirong, Wu, Yingsong, Li, Ji-Liang. 2021. Tspan5 promotes epithelial-mesenchymal transition and tumour metastasis of hepatocellular carcinoma by activating Notch signalling. In Molecular oncology, 15, 3184-3202. doi:10.1002/1878-0261.12980. https://pubmed.ncbi.nlm.nih.gov/33955149/
3. Ho, Ming-Fen, Zhang, Cheng, Zhang, Lingxin, Li, Hu, Weinshilboum, Richard. 2020. TSPAN5 influences serotonin and kynurenine: pharmacogenomic mechanisms related to alcohol use disorder and acamprosate treatment response. In Molecular psychiatry, 26, 3122-3133. doi:10.1038/s41380-020-0855-9. https://pubmed.ncbi.nlm.nih.gov/32753686/
4. Moretto, Edoardo, Longatti, Anna, Murru, Luca, Thoumine, Olivier, Passafaro, Maria. . TSPAN5 Enriched Microdomains Provide a Platform for Dendritic Spine Maturation through Neuroligin-1 Clustering. In Cell reports, 29, 1130-1146.e8. doi:10.1016/j.celrep.2019.09.051. https://pubmed.ncbi.nlm.nih.gov/31665629/
5. Moretto, Edoardo, Miozzo, Federico, Longatti, Anna, Cingolani, Lorenzo A, Passafaro, Maria. 2023. The tetraspanin TSPAN5 regulates AMPAR exocytosis by interacting with the AP4 complex. In eLife, 12, . doi:10.7554/eLife.76425. https://pubmed.ncbi.nlm.nih.gov/36795458/
6. He, Peirong, Wang, Suihai, Zhang, Xuefeng, Jiang, Bo, Li, Ji-Liang. . Tspan5 is an independent favourable prognostic factor and suppresses tumour growth in gastric cancer. In Oncotarget, 7, 40160-40173. doi:10.18632/oncotarget.9514. https://pubmed.ncbi.nlm.nih.gov/27223087/
7. Schreyer, Laura, Mittermeier, Constanze, Franz, Miriam J, Aigner, Achim, Muehlich, Susanne. 2021. Tetraspanin 5 (TSPAN5), a Novel Gatekeeper of the Tumor Suppressor DLC1 and Myocardin-Related Transcription Factors (MRTFs), Controls HCC Growth and Senescence. In Cancers, 13, . doi:10.3390/cancers13215373. https://pubmed.ncbi.nlm.nih.gov/34771537/
품질 관리 기준
정자 검사
동결 보존 전: 정자 농도 측정 및 정자 생존율 평가.
동결 보존 후: 각 배치에서 동결 보존된 정자 바이알 1개를 선택하여 체외수정(in vitro fertilization)에 사용합니다.
Environmental Standards:
SPFAvailable Region:
GlobalSource:
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