Mir195a-KO Mouse
Common Name
Mir195a-KO
제품 ID
S-KO-19048
Backgroud
C57BL/6JCya
품종 계통계통 ID
KOCMP-387190-Mir195a-B6J-VA
상태
이 마우스 계통을 논문에서 사용할 경우, “Mir195a-KO Mouse (카탈로그 번호 S-KO-19048)은 Cyagen에서 구입하였습니다.”라고 명시해 주시기 바랍니다.
구매 가능한 제품 종류
연령
Genotype
성별
수량
표준 제공 조건은 최소 3마리의 이형접합(heterozygous) 보균자를 보장합니다. 동형접합(homozygous) 보균자 및/또는 특정 성별에 대한 브리딩 서비스도 제공됩니다.
기본 정보
품종 계통
Mir195a-KO
품종 계통계통 ID
KOCMP-387190-Mir195a-B6J-VA
유전자명
제품 ID
S-KO-19048
유전자 별칭
Mir195, Mirn195, mir-195a, mmu-mir-195, mmu-mir-195a
배경
C57BL/6JCya
NCBI ID
변형 내용
Conventional knockout
염색체
Chr 11
Phenotype
Datasheet
적용 분야
--
품종 계통 설명
Ensembl 전사체 ID
ENSMUST00000083477
NCBI 전사체 ID
NR_029581
타겟 영역
Exon 1
유효 영역 크기
~0.1 kb
유전자 연구 개요
Mir195a is a microRNA that plays crucial roles in multiple biological processes. It is involved in regulating cell proliferation, apoptosis, and metabolism, and is associated with pathways like the Hippo signaling pathway. MicroRNAs like Mir195a are important for maintaining normal cellular functions and their dysregulation can lead to various pathological conditions [1,4].
In miR-195a KO mice, cognitive impairment and reduced dendritic spine density were observed, indicating its role in age-associated cognitive functions. Sema3D was identified as a direct target of miR-195a, and its age-dependent increase in rodent brains suggested a possible contribution to age-associated neurodegeneration [2].
In mice prenatally exposed to vinclozolin, increased miR-195a levels in the offspring's penises, testes, uteri, and ovaries were found. This led to activation of the Hippo pathway, down-regulation of the androgen receptor in penises, and ultimately caused penile, testicular, uterine, and ovarian damage and malformations [1,4].
In Dicer-deficient macrophages, restoring miR-195a expression could rescue mitochondrial fatty acid oxidative metabolism, highlighting its role in macrophage activation and atherosclerosis prevention [3]. Also, miR-195a inhibits adipocyte differentiation by targeting Zfp423, suggesting its involvement in obesity and metabolic diseases [5].
In intervertebral disk degeneration, silencing of lncRNA NEAT1 upregulates miR-195a to attenuate the process via the BAX/BAK pathway [6]. Benzothiazole-based conjugates affect miR-195a expression, causing cell cycle arrest and apoptosis in hepatocellular carcinoma cells [7].
In conclusion, Mir195a is essential in various biological processes and disease conditions. Gene knockout mouse models have been instrumental in revealing its functions in age-associated cognitive impairment, reproductive organ development, macrophage-related atherosclerosis, adipocyte differentiation, intervertebral disk degeneration, and hepatocellular carcinoma. Understanding Mir195a provides insights into disease mechanisms and potential therapeutic targets.
References：
1. Yu, Haiming, Yang, Jinru, Zhang, Yujing, Yan, Zhengli, Zhu, Yongfei. 2021. Vinclozolin-induced mouse penile malformation and "small testis" via miR132, miR195a together with the Hippo signaling pathway. In Toxicology, 460, 152842. doi:10.1016/j.tox.2021.152842. https://pubmed.ncbi.nlm.nih.gov/34182078/
2. Chen, Chien-Yuan, Chao, Yung-Mei, Cho, Ching-Chang, Chan, Julie Y H, Juo, Suh-Hang H. 2023. Cerebral Semaphorin3D is a novel risk factor for age-associated cognitive impairment. In Cell communication and signaling : CCS, 21, 140. doi:10.1186/s12964-023-01158-5. https://pubmed.ncbi.nlm.nih.gov/37316917/
3. Wei, Yuanyuan, Corbalán-Campos, Judit, Gurung, Rashmi, Zimmer, Ralf, Schober, Andreas. . Dicer in Macrophages Prevents Atherosclerosis by Promoting Mitochondrial Oxidative Metabolism. In Circulation, 138, 2007-2020. doi:10.1161/CIRCULATIONAHA.117.031589. https://pubmed.ncbi.nlm.nih.gov/29748186/
4. Fu, Hu, Yang, Jinru, Xin, Bingyan, Yan, Zhengli, Zhu, Yongfei. 2023. Accentuated Hippo pathway and elevated miR-132 and miR-195a lead to changes of uteri and ovaries in offspring mice following prenatal exposure to vinclozolin. In Reproductive toxicology (Elmsford, N.Y.), 116, 108335. doi:10.1016/j.reprotox.2023.108335. https://pubmed.ncbi.nlm.nih.gov/36642194/
5. Yun, Ui Jeong, Song, No-Joon, Yang, Dong Kwon, Park, Kye Won, Kang, Hara. . miR-195a inhibits adipocyte differentiation by targeting the preadipogenic determinator Zfp423. In Journal of cellular biochemistry, 116, 2589-97. doi:10.1002/jcb.25204. https://pubmed.ncbi.nlm.nih.gov/25903991/
6. Tang, Ning, Dong, Yulei, Liu, Jiaming, Zhao, Hong. 2020. Silencing of Long Non-coding RNA NEAT1 Upregulates miR-195a to Attenuate Intervertebral Disk Degeneration via the BAX/BAK Pathway. In Frontiers in molecular biosciences, 7, 147. doi:10.3389/fmolb.2020.00147. https://pubmed.ncbi.nlm.nih.gov/32850952/
7. Pushpavalli, Sncvl, Ramaiah, M Janaki, Srinivas, Ch, Bhadra, Utpal, Bhadra, Manika Pal. 2011. Effect of Benzothiazole based conjugates in causing apoptosis by Regulating p53, PTEN and MAP Kinase proteins affecting miR-195a and miR-101-1. In Cancer cell international, 11, 36. doi:10.1186/1475-2867-11-36. https://pubmed.ncbi.nlm.nih.gov/22035408/
품질 관리 기준
정자 검사
동결 보존 전: 정자 농도 측정 및 정자 생존율 평가.
동결 보존 후: 각 배치에서 동결 보존된 정자 바이알 1개를 선택하여 체외수정(in vitro fertilization)에 사용합니다.
Environmental Standards:
SPFAvailable Region:
GlobalSource:
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