Mapk12-KO Mouse
Common Name
Mapk12-KO
제품 ID
S-KO-19494
Backgroud
C57BL/6JCya
품종 계통계통 ID
KOCMP-29857-Mapk12-B6J-VB
상태
이 마우스 계통을 논문에서 사용할 경우, “Mapk12-KO Mouse (카탈로그 번호 S-KO-19494)은 Cyagen에서 구입하였습니다.”라고 명시해 주시기 바랍니다.
구매 가능한 제품 종류
연령
Genotype
성별
수량
표준 제공 조건은 최소 3마리의 이형접합(heterozygous) 보균자를 보장합니다. 동형접합(homozygous) 보균자 및/또는 특정 성별에 대한 브리딩 서비스도 제공됩니다.
기본 정보
품종 계통
Mapk12-KO
품종 계통계통 ID
KOCMP-29857-Mapk12-B6J-VB
유전자명
제품 ID
S-KO-19494
유전자 별칭
Erk6, Sapk3, Prkm12, P38gamma
배경
C57BL/6JCya
NCBI ID
변형 내용
Conventional knockout
염색체
Chr 15
Phenotype
Datasheet
적용 분야
--
품종 계통 설명
Ensembl 전사체 ID
ENSMUST00000088827
NCBI 전사체 ID
NM_013871
타겟 영역
Exon 3
유효 영역 크기
~1.7 kb
유전자 연구 개요
Mapk12, also known as P38γ, is a member of the p38 MAPK family. It is involved in various cellular processes, such as cell proliferation, apoptosis, stress responses, and cancer development, and plays a crucial role in the regulation of the innate immune response [2,4,5]. Genetic models, like gene knockout mouse models, are valuable for studying its functions.
In osteoarthritis (OA), downregulation of Mapk12 was observed in H2O2-or IL-1β-stimulated cells and OA-related tissues. Over-expression of Mapk12 promoted cell proliferation and cartilage anabolism, inhibited cell senescence and cartilage catabolism, and relieved joint pain in relevant cell models and DMM mice, indicating its involvement in OA development through modulating chondrocyte senescence [1].
In cancer, pan-cancer analysis showed Mapk12 was overexpressed in multiple cancer types, and its upregulated mRNA expression was related to a worse prognosis. Knockdown of Mapk12 inhibited thyroid carcinoma cell proliferation, and its gene enrichment was related to cell proliferation and the tumor immune microenvironment. It was also closely related to immune checkpoint, microsatellite instability, and tumor mutational burden, affecting tumor sensitivity to immunotherapy [2].
In diffuse large B-cell lymphoma (DLBCL), high expression of Mapk12 was significantly correlated with shorter progression-free survival and overall survival, and was involved in the regulation of type II interferon production and lymphocyte proliferation [6].
In osteosarcoma, miR-187 inhibited tumor growth and invasion by directly targeting Mapk12 [7].
In ovine ovarian granulosa cells, high prolactin concentration promoted apoptosis and inhibited steroid hormone secretion by upregulating Mapk12 [3].
In conclusion, Mapk12 is a key regulator in multiple biological processes and diseases. Studies using gene-based models have revealed its role in OA, various cancers, and ovarian granulosa cell apoptosis. Understanding Mapk12 functions can provide potential therapeutic targets for these diseases.
References：
1. Geng, Nana, Xian, Menglin, Deng, Lin, Bai, Zhixun, Guo, Fengjin. 2024. Targeting the senescence-related genes MAPK12 and FOS to alleviate osteoarthritis. In Journal of orthopaedic translation, 47, 50-62. doi:10.1016/j.jot.2024.06.008. https://pubmed.ncbi.nlm.nih.gov/39007035/
2. Wang, Jinju, Song, Zhe, Ren, Li, Gu, Yi, Feng, Chao. 2022. Pan-cancer analysis supports MAPK12 as a potential prognostic and immunotherapeutic target in multiple tumor types, including in THCA. In Oncology letters, 24, 445. doi:10.3892/ol.2022.13565. https://pubmed.ncbi.nlm.nih.gov/36420075/
3. Yang, Ruochen, Duan, Chunhui, Zhang, Shuo, Liu, Yueqin, Zhang, Yingjie. 2023. Prolactin Regulates Ovine Ovarian Granulosa Cell Apoptosis by Affecting the Expression of MAPK12 Gene. In International journal of molecular sciences, 24, . doi:10.3390/ijms241210269. https://pubmed.ncbi.nlm.nih.gov/37373417/
4. Escós, Alejandra, Diaz-Mora, Ester, Pattison, Michael, Ley, Steven C, Cuenda, Ana. 2023. p38γ and p38δ modulate innate immune response by regulating MEF2D activation. In eLife, 12, . doi:10.7554/eLife.86200. https://pubmed.ncbi.nlm.nih.gov/37458356/
5. Phan, Thuy, Zhang, Xu Hannah, Rosen, Steven, Melstrom, Laleh G. 2023. P38 kinase in gastrointestinal cancers. In Cancer gene therapy, 30, 1181-1189. doi:10.1038/s41417-023-00622-1. https://pubmed.ncbi.nlm.nih.gov/37248432/
6. Liu, Yue, Zhang, Han, Zhao, Shu, Zhang, Yue. 2024. A retrospective analysis of the clinicopathological features and prognostic value of MAPK12 protein expression in diffuse large B-cell lymphoma. In Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico, 26, 2966-2978. doi:10.1007/s12094-024-03515-3. https://pubmed.ncbi.nlm.nih.gov/38773060/
7. Cui, Chengliang, Shi, Xiaoyu. 2017. miR-187 inhibits tumor growth and invasion by directly targeting MAPK12 in osteosarcoma. In Experimental and therapeutic medicine, 14, 1045-1050. doi:10.3892/etm.2017.4624. https://pubmed.ncbi.nlm.nih.gov/28810556/
품질 관리 기준
정자 검사
동결 보존 전: 정자 농도 측정 및 정자 생존율 평가.
동결 보존 후: 각 배치에서 동결 보존된 정자 바이알 1개를 선택하여 체외수정(in vitro fertilization)에 사용합니다.
Environmental Standards:
SPFAvailable Region:
GlobalSource:
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