Morrbid-KO Mouse
Common Name
Morrbid-KO
제품 ID
S-KO-20377
Backgroud
C57BL/6JCya
품종 계통계통 ID
KOCMP-100043424-Morrbid-B6J-VA
상태
이 마우스 계통을 논문에서 사용할 경우, “Morrbid-KO Mouse (카탈로그 번호 S-KO-20377)은 Cyagen에서 구입하였습니다.”라고 명시해 주시기 바랍니다.
구매 가능한 제품 종류
연령
Genotype
성별
수량
표준 제공 조건은 최소 3마리의 이형접합(heterozygous) 보균자를 보장합니다. 동형접합(homozygous) 보균자 및/또는 특정 성별에 대한 브리딩 서비스도 제공됩니다.
기본 정보
품종 계통
Morrbid-KO
품종 계통계통 ID
KOCMP-100043424-Morrbid-B6J-VA
유전자명
제품 ID
S-KO-20377
유전자 별칭
Cytor, Gm14005, Mir4435-2hg
배경
C57BL/6JCya
NCBI ID
변형 내용
Conventional knockout
염색체
Chr 2
Phenotype
Datasheet
적용 분야
--
품종 계통 설명
Ensembl 전사체 ID
ENSMUST00000225087
NCBI 전사체 ID
--
타겟 영역
Exon 1~2
유효 영역 크기
~181.9 kb
유전자 연구 개요
Morrbid, also known as Myeloid RNA regulator of Bim-induced death, is a long noncoding RNA (lncRNA) that has emerged as a significant regulator in multiple biological processes. It is involved in pathways related to apoptosis, cell differentiation, and splicing regulation, thereby playing a crucial role in maintaining normal cellular functions and influencing disease development [1,2,3,4,5,6,8]. Genetic models, such as knockout (KO) mouse models, have been instrumental in uncovering its functions.
In the context of acute myocardial infarction (AMI), Morrbid expression is increased in cardiomyocytes under hypoxia or oxidative stress and in mouse hearts with AMI. Overexpression of Morrbid reduces infarct size and cardiac dysfunction, while cardiomyocyte-specific Morrbid-KO (Morrbidfl/fl/Myh6-Cre) mice show deteriorated infarct size and cardiac function. This indicates that Morrbid protects hearts from AMI via anti-apoptosis through its target gene serpine1 [1].
In the case of monocyte-macrophage differentiation and atherogenesis, Morrbid expression increases during differentiation. Morrbid knockdown inhibits this differentiation and macrophage activity, and Morrbid-overexpressing mice show enhanced monocytes/macrophages recruitment and atherosclerotic lesion formation, while monocyte/macrophage-specific Morrbid knockout has the opposite effect, suggesting its role in atherogenesis [2].
In the context of leukemia, loss of Morrbid in mouse models of juvenile myelomonocytic leukemia (JMML) driven by the Shp2E76K/+ mutation and in models of hyperglycemia-induced leukemia corrects myeloid and erythroid cell abnormalities, prolongs survival, and mitigates the disease, indicating Morrbid's contribution to leukemia pathogenesis [3,7].
In conclusion, Morrbid is a key lncRNA that regulates apoptosis, cell differentiation, and splicing, influencing diseases such as AMI, atherosclerosis, and leukemia. The use of Morrbid KO/CKO mouse models has provided valuable insights into its role in these disease areas, highlighting its potential as a therapeutic target for ischemic heart diseases and myeloid neoplasms [1,2,3,7].
References：
1. Yu, Yang, Yang, Haiqiong, Li, Qiuting, Wang, Xiaobin, Zhang, Chunxiang. 2023. Stress-enhanced cardiac lncRNA Morrbid protects hearts from acute myocardial infarction. In JCI insight, 8, . doi:10.1172/jci.insight.165568. https://pubmed.ncbi.nlm.nih.gov/37432746/
2. Xiang, Di, Jiang, Lei, Yuan, Qiong, Qin, Gangjian, Zhang, Chunxiang. 2023. Leukocyte-Specific Morrbid Promotes Leukocyte Differentiation and Atherogenesis. In Research (Washington, D.C.), 6, 0187. doi:10.34133/research.0187. https://pubmed.ncbi.nlm.nih.gov/37426471/
3. Cai, Zhigang, Zhang, Chi, Kotzin, Jonathan J, Henao-Mejia, Jorge, Kapur, Reuben. . Role of lncRNA Morrbid in PTPN11(Shp2)E76K-driven juvenile myelomonocytic leukemia. In Blood advances, 4, 3246-3251. doi:10.1182/bloodadvances.2020002123. https://pubmed.ncbi.nlm.nih.gov/32697817/
4. Cai, Zhigang, Aguilera, Fabiola, Ramdas, Baskar, Henao-Mejia, Jorge, Kapur, Reuben. . Targeting Bim via a lncRNA Morrbid Regulates the Survival of Preleukemic and Leukemic Cells. In Cell reports, 31, 107816. doi:10.1016/j.celrep.2020.107816. https://pubmed.ncbi.nlm.nih.gov/32579941/
5. Kotzin, Jonathan J, Iseka, Fany, Wright, Jasmine, Wherry, E John, Henao-Mejia, Jorge. 2019. The long noncoding RNA Morrbid regulates CD8 T cells in response to viral infection. In Proceedings of the National Academy of Sciences of the United States of America, 116, 11916-11925. doi:10.1073/pnas.1819457116. https://pubmed.ncbi.nlm.nih.gov/31138702/
6. Fefilova, Anna, Melnikov, Pavel, Prikazchikova, Tatiana, Sergeeva, Olga, Zatsepin, Timofei S. 2020. Murine Long Noncoding RNA Morrbid Contributes in the Regulation of NRAS Splicing in Hepatocytes In Vitro. In International journal of molecular sciences, 21, . doi:10.3390/ijms21165605. https://pubmed.ncbi.nlm.nih.gov/32764370/
7. Cai, Zhigang, Lu, Xiaoyu, Zhang, Chi, Haneline, Laura, Kapur, Reuben. . Hyperglycemia cooperates with Tet2 heterozygosity to induce leukemia driven by proinflammatory cytokine-induced lncRNA Morrbid. In The Journal of clinical investigation, 131, . doi:10.1172/JCI140707. https://pubmed.ncbi.nlm.nih.gov/33090974/
8. Kotzin, Jonathan J, Spencer, Sean P, McCright, Sam J, Flavell, Richard A, Henao-Mejia, Jorge. 2016. The long non-coding RNA Morrbid regulates Bim and short-lived myeloid cell lifespan. In Nature, 537, 239-243. doi:10.1038/nature19346. https://pubmed.ncbi.nlm.nih.gov/27525555/
품질 관리 기준
정자 검사
동결 보존 전: 정자 농도 측정 및 정자 생존율 평가.
동결 보존 후: 각 배치에서 동결 보존된 정자 바이알 1개를 선택하여 체외수정(in vitro fertilization)에 사용합니다.
Environmental Standards:
SPFAvailable Region:
GlobalSource:
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