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huPBMC-NKG Mouse
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huPBMC-NKG Mouse
제품명
huPBMC-NKG Mouse
제품 ID
C001329
품종 계통
huPBMC-NOD.Cg-PrkdcscidIl2rgem1cya/Cya
Backgroud
NKG
상태
이 마우스 계통을 논문에서 사용할 경우, “huPBMC-NKG Mouse (카탈로그 번호 C001329)은 Cyagen에서 구입하였습니다.”라고 명시해 주시기 바랍니다.
Immunodeficient Mice
Humanized Immune System Models
구매 가능한 제품 종류
연령
Genotype
성별
수량
표준 제공 조건은 최소 3마리의 이형접합(heterozygous) 보균자를 보장합니다. 동형접합(homozygous) 보균자 및/또는 특정 성별에 대한 브리딩 서비스도 제공됩니다.
가격 문의
Immunodeficient Mice
Humanized Immune System Models
기본 정보
검증 데이터
관련 자료
기본 정보
유전자명
유전자 별칭
gc, p64, [g]c, CD132, gamma(c)
NCBI ID
염색체
Chr X
MGI ID
Datasheet
품종 계통 설명
NKG mice (Product number: C001316) are a kind of severe immunodeficient mice generated by Cyagen through deleting the Il2rg gene from NOD-Scid mice. NKG mice exhibit deficiency of mature T cells, B cells, and functional NK cells, reduced complement activity, and weak phagocytosis of human-derived cells by macrophages, which are well suited for transplantation of human hematopoietic stem cells (HSC), peripheral blood mononuclear cells (PBMC), adult stem cells and tissues, and patient-derived xenograft (PDX) to obtain the humanized mice with the human immune system.
PBMC humanized mice refer to the immune system humanized mouse model constructed by transplanting human peripheral blood mononuclear cells (PBMC) into immunodeficient mice (such as NKG). NKG mice transplanted with PBMC (huPBMC-NKG) have high efficiency and fast immune reconstitution. The average proportion of human CD45+ in peripheral blood (PB) is about 50% after 3 weeks of transplantation, and the reconstituted immune cells are mainly lymphoid T cells. Human T cells generated after PBMC injection will attack mouse recipient cells, resulting in graft-versus-host disease (GvHD), so this model can be used to evaluate GvHD drugs.
변형 전략
Female NKG mice aged 6 weeks were selected to inject PBMC for the construction of the huPBMC-NKG model, and the construction period was 2-3 weeks.
응용 분야
Short-term studies requiring mature T cells in the fields of tumor immunity, Hematopoiesis, and gene therapy for hematologic and infectious diseases.
검증 데이터
1. Survival curve
Around 35 days post engraftment, huPBMC-NKG mice gradually began to die as Graft-versus-Host Disease (GvHD) appeared. After 60 days of transplantation, about 20% of the mice were still alive.
Figure 1. The survival curve of huPBMC-NKG mice.
2. The reconstruction status of the immune system.
（1）Detection of Human CD45+ Cells in Peripheral Blood (PB)
After transplantation of human PBMCs, the content of human leukocytes in the peripheral blood gradually increased. After 3 weeks, the average proportion of human CD45+ cells exceeded 40% and the reconstruction speed was relatively fast. After transplantation, the humanization ratio remained at a high level for 3-6 weeks.
Figure 2. The proportion of human CD45+ cells in peripheral blood (PB) of huPBMC-NKG mice.
（2）Detection of Human CD3+ Cells in Peripheral Blood (PB)
About 95%- 100% of hCD45+ cells in huPBMC-NKG mice belong to hCD3+ T cells 3 weeks after human PBMC transplantation, indicating that the reconstruction of the human immune system in huPBMC-NKG mice was dominated by T cells.
Figure 3. The proportion of human CD3+ T cells in the peripheral blood of huPBMC-NKG mice.
（3）The Graft-versus-Host Disease (GvHD)
GvHD symptoms begin to appear around 28d. Mice lost a significant amount of body weight and the body weight was reduced by more than 20% after 45d.
Figure 4. GvHD scores and body weight changes in NKG mice after PBMC transplantation.
관련 자료
1:1 상담
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